Identification and phylogenetic of cysteine peptidase genes from the bamboo pest Hippotiscus dorsalis, based on RNA-seq and PacBio Iso-Seq

Abstract

One of the insects that cause the most damage to Moso bamboos in the southern regions of China is Hippotiscus dorsalis (Hemiptera: Pentatomidae). However, the study of molecular and growth development analysis is limited because its genetic resources are still in the blank stage. In order to improve our knowledge of the molecular traits of this insect pest, we first generated the transcriptome from H dorsalis using PacBio Iso-Seq. With an average length of 1495 bp, 207,663 nonredundant full-length reads were produced. Using five databases, we annotated 36,238 transcripts: Uniprot (24,388), GO (1160), NR (24,110), Pfam (9333), and KEGG (14,497). According to aligning of each transcript in the NR database, most of them (85.147 %) were annotated into the functional genes from Halyomorpha halys. In the meantime, we discovered 33,689 long noncoding RNAs (lncRNAs), 4408 transcription factors (TFs), and 586 alternative splicing (AS) events, which involved in transcriptional regulation. Additionally, the candidate cathepsin genes from the bamboo pest were examined in order to comprehend how they adapted to their host. To sum up, our findings offer a fresh resource for comprehensive transcriptional data and insights into the genetics and gene expression of H dorsalis.