Journal of Asia-pacific Entomology最新文献

英文中文

Molecular identification and functional analysis of the putative ecdysone receptor in Spodoptera litura

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-03-01 DOI: 10.1016/j.aspen.2025.102390

Ziqian Tong , Huixuan Shi , Zhuo Liu , Donghai Zhang , Dandan Li , Yunchao Kan , Huili Qiao

20-hydroxyedysone (20E) is an important hormone that regulates the process of molting and metamorphosis in insects. Ecdysone receptor (EcR) is one of the most important primary nuclear receptor for 20E. To clarify the essential function of EcR during the development of Spodoptera litura, the gene encoding the putative ecdysone receptor isoform A (SlEcRA) was cloned and verified. Multiple sequence comparisons and the subsequent phylogenetic study indicated that SlEcRA protein exhibited a high degree of conservation and significant homology with EcRA proteins in other insects. Spatiotemporal expression analysis showed that SlEcRs were predominantly expressed during the larval molting and prepupa stages, with the highest expression in fat body. Moreover, the expression of SlEcRs were induced by 20E in fat body, midgut and integument. Silencing of SlEcRs led to increased mortality and decreased larval weight during larval development. A combination treatment of dsSlEcRs and low-concentration emamectin benzoate effectively increased larvae mortality at 48 h. Furthermore, the larval mortality of Chi-dsSlEcRs nanoparticles by epidermal infiltration was much higher than the naked dsSlEcRs by injection. Co-delivery of Chi-dsSlEcRs and emamectin benzoate exhibited shorter action time and higher mortality rate on larvae. These findings not only highlight the potential of the putative SlEcR as an RNA interference target for S. litura control, but also provide a new idea for reducing the reliance on chemical insecticides in agricultural pest control.

{"title":"Molecular identification and functional analysis of the putative ecdysone receptor in Spodoptera litura","authors":"Ziqian Tong , Huixuan Shi , Zhuo Liu , Donghai Zhang , Dandan Li , Yunchao Kan , Huili Qiao","doi":"10.1016/j.aspen.2025.102390","DOIUrl":"10.1016/j.aspen.2025.102390","url":null,"abstract":"<div><div>20-hydroxyedysone (20E) is an important hormone that regulates the process of molting and metamorphosis in insects. Ecdysone receptor (EcR) is one of the most important primary nuclear receptor for 20E. To clarify the essential function of EcR during the development of <em>Spodoptera litura</em>, the gene encoding the putative ecdysone receptor isoform A (<em>SlEcRA</em>) was cloned and verified. Multiple sequence comparisons and the subsequent phylogenetic study indicated that SlEcRA protein exhibited a high degree of conservation and significant homology with EcRA proteins in other insects. Spatiotemporal expression analysis showed that <em>SlEcRs</em> were predominantly expressed during the larval molting and prepupa stages, with the highest expression in fat body. Moreover, the expression of <em>SlEcRs</em> were induced by 20E in fat body, midgut and integument. Silencing of <em>SlEcRs</em> led to increased mortality and decreased larval weight during larval development. A combination treatment of dsSlEcRs and low-concentration emamectin benzoate effectively increased larvae mortality at 48 h. Furthermore, the larval mortality of Chi-dsSlEcRs nanoparticles by epidermal infiltration was much higher than the naked dsSlEcRs by injection. Co-delivery of Chi-dsSlEcRs and emamectin benzoate exhibited shorter action time and higher mortality rate on larvae. These findings not only highlight the potential of the putative <em>SlEcR</em> as an RNA interference target for <em>S. litura</em> control, but also provide a new idea for reducing the reliance on chemical insecticides in agricultural pest control.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102390"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of one type of Kazal-type serine protease inhibitor from Silkworms, Bombyx mori (Lepidoptera)

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-02-22 DOI: 10.1016/j.aspen.2025.102386

Jun-li Lv , Qin Li , Jia-wei Jiang , Min Ji Park , Kwang Sik Lee , Xue-yang Wang , Zuo-min Shao , Byung Rae Jin

The silk of silkworm, Bombyx mori, consists mainly of proteins, which contain some small proteins with specialized functions that confer special properties to silk, such as antimicrobial properties. Serine protease inhibitors (SPIs) are one of them, and the types that have been identified are seroin, serpin, Kunitz type, and Kazal type, but the function of the Kunitz/Kazal type is not known. As compared to others, Kazal molecular weight is relatively minimal. In this study, we focused on the function of a Kazal-type inhibitor BmSPI2, and its sequence characterization and expression profile were first refined. To clarify its function, BmSPI2 recombinant protein was expressed in sf9 cells using baculovirus expression system. Further testing revealed that BmSPI2 could inhibit trypsin, α-chymotrypsin, and plasmin, whereas has only some effect on proteinase K. This suggests that BmSPI2 has significant serine protease inhibitory activity. In-depth analysis revealed that BmSPI2 also effectively inhibited fibrinogen degradation mediated by fibrinolytic enzymes. Finally, we examined its antimicrobial-related functions using prepared antibodies and found that BmSPI2 was also able to directly bind to bacteria and fungi to inhibit their proliferation. The results of the study enriched the function of BmSPI2 and laid a theoretical foundation for its development and utilization.

{"title":"The function of one type of Kazal-type serine protease inhibitor from Silkworms, Bombyx mori (Lepidoptera)","authors":"Jun-li Lv , Qin Li , Jia-wei Jiang , Min Ji Park , Kwang Sik Lee , Xue-yang Wang , Zuo-min Shao , Byung Rae Jin","doi":"10.1016/j.aspen.2025.102386","DOIUrl":"10.1016/j.aspen.2025.102386","url":null,"abstract":"<div><div>The silk of silkworm, <em>Bombyx mori</em>, consists mainly of proteins, which contain some small proteins with specialized functions that confer special properties to silk, such as antimicrobial properties. Serine protease inhibitors (SPIs) are one of them, and the types that have been identified are seroin, serpin, Kunitz type, and Kazal type, but the function of the Kunitz/Kazal type is not known. As compared to others, Kazal molecular weight is relatively minimal. In this study, we focused on the function of a Kazal-type inhibitor BmSPI2, and its sequence characterization and expression profile were first refined. To clarify its function, BmSPI2 recombinant protein was expressed in sf9 cells using baculovirus expression system. Further testing revealed that BmSPI2 could inhibit trypsin, <em>α-</em>chymotrypsin, and plasmin, whereas has only some effect on proteinase K. This suggests that BmSPI2 has significant serine protease inhibitory activity. In-depth analysis revealed that BmSPI2 also effectively inhibited fibrinogen degradation mediated by fibrinolytic enzymes. Finally, we examined its antimicrobial-related functions using prepared antibodies and found that BmSPI2 was also able to directly bind to bacteria and fungi to inhibit their proliferation. The results of the study enriched the function of BmSPI2 and laid a theoretical foundation for its development and utilization.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102386"},"PeriodicalIF":1.1,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel splice variants of the cytochrome P450 9A19 gene in the domestic silkworm Bombyx mori

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-02-10 DOI: 10.1016/j.aspen.2025.102382

Jin Ha Yun, Seung-Won Park

CYP9 gene family members participate in neutralization pathways associated with pesticide resistance. Alternative splicing ultimately plays a role in increasing the diversity of the proteome through increasing transcriptome diversity. Here, we characterized various novel CYP9A19 isoforms in Bombyx mori. We first identified an exon 4 deletion isoform that was 203 bp smaller than expected due to a deletion of exon 4 from the protein coding sequence (CDS) region of the CYP9A19 cDNA. Within the CYP9A19 cDNA, there is a 176 bp 5′-untranslated region (UTR); reverse transcription-polymerase chain reaction results showed 74 bp lower and 122 bp higher molecular-weight products compared with the expected size from the 5′-UTR of CYP9A19 cDNA in B. mori. Additionally, we found a new sequence (5′-GTCTCAGGTTCAGGGCTCTAGCAATTTTTCACAG-3′) in the 5′-UTR of CYP9A19. Currently, our understanding of the various CYP9A19 isoform functions in pesticide degradation and resistance and insect development and differentiation stages remains limited. Therefore, additional studies on the alternative splicing process are needed to verify the biological role of the novel splicing variant of CYP9A19 in B. mori. Our findings may provide insight into the genetic background of the processes and mechanisms underlying the stages of insect development and differentiation in lepidoptera species.

{"title":"Novel splice variants of the cytochrome P450 9A19 gene in the domestic silkworm Bombyx mori","authors":"Jin Ha Yun, Seung-Won Park","doi":"10.1016/j.aspen.2025.102382","DOIUrl":"10.1016/j.aspen.2025.102382","url":null,"abstract":"<div><div><em>CYP9</em> gene family members participate in neutralization pathways associated with pesticide resistance. Alternative splicing ultimately plays a role in increasing the diversity of the proteome through increasing transcriptome diversity. Here, we characterized various novel <em>CYP9A19</em> isoforms in <em>Bombyx mori</em>. We first identified an exon 4 deletion isoform that was 203 bp smaller than expected due to a deletion of exon 4 from the protein coding sequence (CDS) region of the <em>CYP9A19</em> cDNA. Within the <em>CYP9A19</em> cDNA, there is a 176 bp 5′-untranslated region (UTR); reverse transcription-polymerase chain reaction results showed 74 bp lower and 122 bp higher molecular-weight products compared with the expected size from the 5′-UTR of <em>CYP9A19</em> cDNA in <em>B. mori</em>. Additionally, we found a new sequence (5′-GTCTCAGGTTCAGGGCTCTAGCAATTTTTCACAG-3′) in the 5′-UTR of <em>CYP9A19</em>. Currently, our understanding of the various <em>CYP9A19</em> isoform functions in pesticide degradation and resistance and insect development and differentiation stages remains limited. Therefore, additional studies on the alternative splicing process are needed to verify the biological role of the novel splicing variant of <em>CYP9A19</em> in <em>B. mori</em>. Our findings may provide insight into the genetic background of the processes and mechanisms underlying the stages of insect development and differentiation in lepidoptera species.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102382"},"PeriodicalIF":1.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A remarkable new species of the genus Dichodontus Burmeister, 1847 (Coleoptera: Scarabaeidae: Dynastinae) from Thailand and Myanmar, with discussions on some Sundaic species

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-02-04 DOI: 10.1016/j.aspen.2025.102381

Georgi A. Geshev , Wuttipon Pathomwattananurak , Kawin Jiaranaisakul

Dichodontus fluctus Geshev, Pathomwattananurak & Jiaranaisakul, new species from Thailand and Myanmar is described and illustrated. Lectotypes and newly identified paralectotypes of Dichodontus punctipennis Arrow, 1937 and Dichodontus grandis Ritsema, 1882, as well as the holotype and a female paratype of Dichodontus crassus Silvestre, 2001 are illustrated. D. punctipennis is newly recorded from Thailand, and D. grandis is recorded from Thailand and Myanmar for the first time. Additionally, previous record of D. crassus from Thailand is discussed and omitted from the distribution.

引用次数: 0

Stethynium empoascae Subba Rao (Hymenoptera: Mymaridae), a newly recorded egg parasitoid of Amrasca biguttula (Ishida) (Hemiptera: Cicadellidae), a pest of okra on Ogasawara (Bonin) Islands, Japan

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-01-23 DOI: 10.1016/j.aspen.2025.102379

Tetsuya Adachi-Hagimori , Serguei V. Triapitsyn , Takuya Endou

The okra leafhopper, Amrasca (Sundapteryx) biguttula (Ishida) (Hemiptera: Cicadellidae), is an economically important pest of okra, Abelmoschus esculentus (L.) Moench (Malvaceae), in southern Japan including Chichijima and Hahajima, Ogasawara (Bonin) Islands. Parasitoids of A. biguttula in the Ogasawara Islands revealed that only one species, Stethynium empoascae Subba Rao (Hymenoptera: Mymaridae), parasitizes its eggs there. A possibility of a biological control program against the okra leafhopper on Ogasawara Islands by introducing its other known egg parasitoids from southern Japan is discussed.

引用次数: 0

New complete mitogenomes of four species in tribe Erythroneurini (Hemiptera: Cicadellidae: Typhlocybinae) from China and their phylogenetic analysis

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-01-22 DOI: 10.1016/j.aspen.2025.102378

Weiwei Ran , Yuanqi Zhao , Jiajia Chen , Ni Zhang , Zhouwei Yuan , Guimei Luo , Yuehua Song

Erythroneurine leafhoppers are important insects in agriculture and forestry. The complete mitochondrial genomes of Motaga mengyangensis, Motaga acicularis, Tautoneura albida, and Tautoneura unicolor, which belong to the tribe Erythroneurini from China, were sequenced for the first time. The lengths of the complete mitogenomes of these four species ranged from 15,361 to 15,856 base pairs, and a typical set of 37 genes was identified in each. Among the 13 protein-coding genes (PCGs), except for the atp8 gene in T. albida and T. unicolor, which started with the codon TTG, the remaining genes all began with typical ATN codons (ATA/ATT/ATC/ATG). The stop codons were TAA, TAG, or an incomplete stop codon T. Utilizing three datasets from the mitochondrial genomes (PCGs, PCGsrRNA, and PCGs12rRNA) of 36 species (including two outgroups), phylogenetic trees of Erythroneurini were constructed via maximum likelihood and Bayesian inference methods. The results revealed that all phylogenetic trees supported the clustering of M. mengyangensis and M. acicularis into one evolutionary branch, as well as the clustering of T. albida and T. unicolor into another. Furthermore, all the results consistently supported the monophyly of genera within Erythroneurini and uniformly demonstrated that the genus Motaga formed a sister group relationship with the genus Empoascanara. Our research fills a crucial gap in the available data on complete mitochondrial genomes within Erythroneurini, laying a solid foundation for future explorations into the molecular aspects of leafhopper evolution and diversity, which further provides reference value for their prevention and control.

{"title":"New complete mitogenomes of four species in tribe Erythroneurini (Hemiptera: Cicadellidae: Typhlocybinae) from China and their phylogenetic analysis","authors":"Weiwei Ran , Yuanqi Zhao , Jiajia Chen , Ni Zhang , Zhouwei Yuan , Guimei Luo , Yuehua Song","doi":"10.1016/j.aspen.2025.102378","DOIUrl":"10.1016/j.aspen.2025.102378","url":null,"abstract":"<div><div>Erythroneurine leafhoppers are important insects in agriculture and forestry. The complete mitochondrial genomes of <em>Motaga mengyangensis</em>, <em>Motaga acicularis</em>, <em>Tautoneura albida</em>, and <em>Tautoneura unicolor</em>, which belong to the tribe Erythroneurini from China, were sequenced for the first time. The lengths of the complete mitogenomes of these four species ranged from 15,361 to 15,856 base pairs, and a typical set of 37 genes was identified in each. Among the 13 protein-coding genes (PCGs), except for the <em>atp8</em> gene in <em>T. albida and T. unicolor</em>, which started with the codon TTG, the remaining genes all began with typical ATN codons (ATA/ATT/ATC/ATG). The stop codons were TAA, TAG, or an incomplete stop codon T. Utilizing three datasets from the mitochondrial genomes (PCGs, PCGsrRNA, and PCGs12rRNA) of 36 species (including two outgroups), phylogenetic trees of Erythroneurini were constructed via maximum likelihood and Bayesian inference methods. The results revealed that all phylogenetic trees supported the clustering of <em>M. mengyangensis</em> and <em>M. acicularis</em> into one evolutionary branch, as well as the clustering of <em>T. albida</em> and <em>T. unicolor</em> into another. Furthermore, all the results consistently supported the monophyly of genera within Erythroneurini and uniformly demonstrated that the genus <em>Motaga</em> formed a sister group relationship with the genus <em>Empoascanara</em>. Our research fills a crucial gap in the available data on complete mitochondrial genomes within Erythroneurini, laying a solid foundation for future explorations into the molecular aspects of leafhopper evolution and diversity, which further provides reference value for their prevention and control.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102378"},"PeriodicalIF":1.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genus Deutereulophus Schulz, 1906 (Hymenoptera: Eulophidae: Eulophinae) with description of three new species from Indonesia

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-01-20 DOI: 10.1016/j.aspen.2025.102377

Rosichon Ubaidillah , Encilia Encilia , Darmawan Darmawan , Hari Nugroho , Hari Sutrisno , Jun-ichi Kojima

The eulophine (Hymenoptera: Eulophidae) genus Deutereulophus Schulz, 1906 occurring in Indonesia is revised, having recognized five species including the following three new species described herein; Deutereulophus thahirae Ubaidillah sp. nov., D. krakatauensis Ubaidillah sp. nov. and D. sancangensis Ubaidillah sp. nov. An identification key to the Indonesian species and a checklist of the world-wide species of Deutereulophus are provided.

urn:lsid:zoobank.org:pub:2519FF5C-F1F6-409F-AF5C-64EA2DB08603.

引用次数: 0

LC-MS profiling reveals metabolic dynamics in Apis mellifera worker bee larvae–pupae transition

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-01-16 DOI: 10.1016/j.aspen.2025.102376

Zhen Li , Shiqing Zhong , Bin Liu , Lizhi Li , Haibo Zhang , Weikun Guan , Dongsheng Guo

Metabolites present in the hemolymph of honey bees play a crucial role in modulating the metamorphic process within the species. However, the precise alterations in metabolite composition, along with the associated variances and regulatory pathways implicated during the larvae-to-pupae metamorphosis of honey bees, remain incompletely elucidated. In this investigation, we gathered hemolymph samples from honey bee larvae of Apis mellifera at three distinct physiological stages-feeding, prepupae, and pupae and subjected them to metabolite analysis utilizing the liquid chromatography-mass spectrometry (LC-MS) technique. Employing principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), we identified significant differential metabolites and conducted a metabolic pathway analysis on those differentially up-regulated during the prepupae and pupae stages. Notably, metabolites up-regulated in the hemolymph of prepupae stage larvae primarily governed glucose metabolism and fat digestion and absorption, while those in pupae stage were involved in regulating chitin and lipopolysaccharide precursor formation, as well as the biosynthesis of phenylalanine, tyrosine, and tryptophan. These findings bear significant implications for advancing our comprehension of the metamorphic processes in honey bees.

{"title":"LC-MS profiling reveals metabolic dynamics in Apis mellifera worker bee larvae–pupae transition","authors":"Zhen Li , Shiqing Zhong , Bin Liu , Lizhi Li , Haibo Zhang , Weikun Guan , Dongsheng Guo","doi":"10.1016/j.aspen.2025.102376","DOIUrl":"10.1016/j.aspen.2025.102376","url":null,"abstract":"<div><div>Metabolites present in the hemolymph of honey bees play a crucial role in modulating the metamorphic process within the species. However, the precise alterations in metabolite composition, along with the associated variances and regulatory pathways implicated during the larvae-to-pupae metamorphosis of honey bees, remain incompletely elucidated. In this investigation, we gathered hemolymph samples from honey bee larvae of <em>Apis mellifera</em> at three distinct physiological stages-feeding, prepupae, and pupae and subjected them to metabolite analysis utilizing the liquid chromatography-mass spectrometry (LC-MS) technique. Employing principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), we identified significant differential metabolites and conducted a metabolic pathway analysis on those differentially up-regulated during the prepupae and pupae stages. Notably, metabolites up-regulated in the hemolymph of prepupae stage larvae primarily governed glucose metabolism and fat digestion and absorption, while those in pupae stage were involved in regulating chitin and lipopolysaccharide precursor formation, as well as the biosynthesis of phenylalanine, tyrosine, and tryptophan. These findings bear significant implications for advancing our comprehension of the metamorphic processes in honey bees.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102376"},"PeriodicalIF":1.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of three new species of Trigonidiidae Saussure, 1874 from China (Orthoptera: Grylloidea)

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-01-15 DOI: 10.1016/j.aspen.2025.102372

Tao Li-Long , Yang Rui-Ying , He Zhi-Xin , Ma Li-Bin

The article describes three new species of Nemobiinae Saussure, 1877 and Trigonidiinae Saussure, 1874 from Yunnan Province, namely Pteronemobius (Stilbonemobius) alboguttatus Ma & Tao sp. nov., Metiochodes wanzhii Ma & Tao sp. nov. and Metiochodes polycomus Ma & Tao sp. nov.. Among them, P. (S.) alboguttatus sp. nov. has a short, rounded shield on the ectoparamere, four internal dorsal spurs on the hind tibia, and many white spots on the head, other morphological characteristics are similar to Pteronemobius (Stilbonemobius) longispinus Gorochov, 1984. Furturemore, M. wanzhii sp. nov. and M. polycomus sp. nov. can be distinguished by the number of main veins in the forewing and the shape of the epiphallus and ectoparamere. Here, we provide a detailed description and photos of the new species.

LSIDurn:lsid:zoobank.org:pub:099A8DC4-0D79-4390-AC28-44E14899EFD8.

{"title":"Description of three new species of Trigonidiidae Saussure, 1874 from China (Orthoptera: Grylloidea)","authors":"Tao Li-Long , Yang Rui-Ying , He Zhi-Xin , Ma Li-Bin","doi":"10.1016/j.aspen.2025.102372","DOIUrl":"10.1016/j.aspen.2025.102372","url":null,"abstract":"<div><div>The article describes three new species of Nemobiinae Saussure, 1877 and Trigonidiinae Saussure, 1874 from Yunnan Province, namely <em>Pteronemobius (Stilbonemobius) alboguttatus</em> Ma & Tao <strong>sp. nov.</strong>, <em>Metiochodes wanzhii</em> Ma & Tao <strong>sp. nov.</strong> and <em>Metiochodes polycomus</em> Ma & Tao <strong>sp. nov.</strong>. Among them, <em>P. (S.) alboguttatus</em> <strong>sp. nov.</strong> has a short, rounded shield on the ectoparamere, four internal dorsal spurs on the hind tibia, and many white spots on the head, other morphological characteristics are similar to <em>Pteronemobius (Stilbonemobius) longispinus</em> Gorochov, 1984. Furturemore, <em>M. wanzhii</em> <strong>sp. nov.</strong> and <em>M. polycomus</em> <strong>sp. nov.</strong> can be distinguished by the number of main veins in the forewing and the shape of the epiphallus and ectoparamere. Here, we provide a detailed description and photos of the new species.</div><div>LSIDurn:lsid:<span><span>zoobank.org</span><svg><path></path></svg></span>:pub:099A8DC4-0D79-4390-AC28-44E14899EFD8.</div></div>","PeriodicalId":15094,"journal":{"name":"Journal of Asia-pacific Entomology","volume":"28 1","pages":"Article 102372"},"PeriodicalIF":1.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A deep learning-based method for silkworm egg counting

IF 1.1 3区农林科学 Q3 ENTOMOLOGY

Journal of Asia-pacific Entomology

Pub Date : 2025-01-11 DOI: 10.1016/j.aspen.2025.102375

Hongkang Shi, Xiao Chen, Minghui Zhu, Linbo Li, Jianmei Wu, Jianfei Zhang

The counting of silkworm eggs is an essential task in the selection and breeding of new silkworm species, as well as in silkworm egg production. Currently, this task mainly relies on manual counting, which poses many challenges such as high workload, low efficiency, and being error-prone. To alleviate these problems, this study proposes a deep learning-based method for silkworm egg counting. Images of silkworm eggs were captured from actual environments and annotated using a labeling tool, resulting in more than 300,000 labeled eggs. A counting network based on the You Only Look Once (YOLOv8n) object detection network is proposed, in which an efficient multi-scale attention (EMA) module is embedded in the extraction block of the original network to enhance feature representation capability and suppress interference. To further improve counting performance, a space-to-depth convolution (SPD-Conv) block is introduced to replace the down-sample layer implemented by convolutional layers with a stride of 2. The proposed network is termed YOLO for silkworm egg counting (YOLO-SEC). Experimental results demonstrate that our YOLO-SEC achieves a recall of 99.50 %, a precision of 98.29 %, an F1-score of 0.99, and an AP of 99.31 % for silkworm eggs on the test set. Meanwhile, YOLO-SEC shows significant performance advantages over the original YOLOv8 (n ∼ x), state-of-the-art networks (YOLOv7-tiny, YOLOv9s, YOLOv10n and YOLOv11n), and related networks (YOLOv8-QR, EDGC-YOLO, Faster-YOLO-AP, YOLOv8-ECFS). This research provides technical support for the breeding and egg production process of silkworms.

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