Solvent-free synthesis of diacylglycerols via enzymatic glycerolysis between edible oils and glycerol catalyzed by self-made immobilized lipase PS@LXTE-1000

Q3 Agricultural and Biological Sciences Oil Crop Science Pub Date : 2024-09-06 DOI:10.1016/j.ocsci.2024.05.003
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Abstract

Diglycerol (DAG) is a structural lipid with the functions to lower body fat accumulation and decrease serum triglyceride level. However, the enzymatic synthesis of DAG is limited by the high-efficient and economic lipases. In this paper, the immobilized lipase PS@LXTE-1000 was self-made by immobilizing the Pseudomomas cepacian lipase on to the hydrophobic microporous resin LXTE-1000. The results indicate that LXTE-1000 was a uniform mesoporous sphere with the mean diameter of 400 ​μm, pore size of 14.6 ​nm, pore volume of 0.5 ​cm3/g and surface area of 126.0 ​m2/g, showing superior structural properties for lipase immobilization. Under the optimal reaction conditions with the molar ratio of rapeseed oil to glycerol being 1:1, adding amount of immobilized lipase being 4%, reaction at 50 ​°C, the highest DAG content of 46.7% was achieved in 3 ​h via enzymatic glycerolysis catalyzed by LXTE-1000. After 7 cycles of reuse, the self-made LXTE-1000 could still retain 78.3% of its initial catalytic ability. Besides, LXTE-1000 was observed to facilitate the DAG production via glycerolysis reaction between glycerol with other seven edible oils including corn oil, sesame oil, peony seed oil, rice bran oil, peanut oil, soybean oil and flaxseed oil. Specifically, the glycerolysis reaction with sesame oil, peony seed oil and rice bran oil even led to the DAG content of 52.1%, 53.3% and 51.2%, respectively, Hence, this paper provide a novel strategy to produce high-efficient and economic immobilized lipases, which shows great potential in the green synthesis of functional lipids such as DAG.
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自制固定化脂肪酶 PS@LXTE-1000 催化食用油与甘油之间的酶促甘油分解,无溶剂合成二酰甘油
二甘油(DAG)是一种结构脂质,具有降低体内脂肪堆积和血清甘油三酯水平的功能。然而,酶法合成 DAG 受限于高效经济的脂肪酶。本文利用疏水性微孔树脂 LXTE-1000 自制了固定化脂肪酶 PS@LXTE-1000。结果表明,LXTE-1000 是一个均匀的介孔球体,平均直径为 400 μm,孔径为 14.6 nm,孔体积为 0.5 cm3/g,比表面积为 126.0 m2/g,显示出固定脂肪酶的优异结构特性。在菜籽油与甘油的摩尔比为 1:1、固定化脂肪酶的添加量为 4%、反应温度为 50 ℃的最佳反应条件下,LXTE-1000 催化的酶促甘油分解反应在 3 小时内达到了 46.7% 的最高 DAG 含量。自制的 LXTE-1000 在重复使用 7 次后,仍能保持 78.3% 的初始催化能力。此外,还观察到 LXTE-1000 可促进甘油与其他七种食用油(包括玉米油、芝麻油、牡丹籽油、米糠油、花生油、大豆油和亚麻籽油)发生甘油分解反应生成 DAG。因此,本文提供了一种生产高效、经济的固定化脂肪酶的新策略,在绿色合成 DAG 等功能脂类方面具有巨大潜力。
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来源期刊
Oil Crop Science
Oil Crop Science Food Science, Plant Science, Agronomy and Crop Science
CiteScore
3.40
自引率
0.00%
发文量
20
审稿时长
74 days
期刊最新文献
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