Influence of light intensity on the responses of seedlings of neotropical tree species to nitrogen source

Abstract

Light intensity plays a crucial role in N uptake and assimilation in plants, but its interaction with different N sources is overlooked. Considering the high energy required for N assimilation, it is hypothesised that low light is critical for the seedling development with both N sources, but with increased light intensity, growing with nitrate (NO₃^-) becomes favourable in relation to ammonium (NH₄⁺). Seedlings of Cecropia pachystachya (pioneer), Guarea kunthiana (shade-tolerant, understory) and Cariniana estrellensis (shade-tolerant, canopy) were grown in hydroponic medium with NO₃^- or NH₄⁺ as the sole N source and subjected to low (LL) or high light (HL) for 60 days. All three species showed a decrease in growth when cultivated with NH₄⁺, compared to NO₃^-, under HL, but not under LL. The decrease in biomass reached 54 % in C. pachystachya, 36 % in G. kunthiana and 26 % in C. estrellensis. Growth reduction was associated with stomatal limitation of photosynthesis and reduced leaf area in C. pachystachya, and with non-stomatal limitation of photosynthesis and oxidative stress in G. kunthiana. Cation uptake was negatively affected by NH₄⁺ in all species. Cariniana estrellensis showed no photosynthetic limitation and showed a higher tolerance to NH₄⁺ under HL in terms of nutrient content. In conclusion, neither N source significantly favors seedling development under LL, while NH₄⁺ is considerably more unfavorable for seedling development than NO₃^- under HL.