首页 > 最新文献

Environmental and Experimental Botany最新文献

英文 中文
Knockout of OsBURP12 enhances salt tolerance in rice seedlings
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2025-02-01 DOI: 10.1016/j.envexpbot.2025.106097
Zengtong Luo , Sijia Yu , Jialing Chen , Qianyi Liu , Mangu Hu , Xiao Yang , Yongxiang Huang , Wuming Xiao
The BURP gene family encodes the BURP domain protein, a type of plant-specific protein that plays an important role in plant development, metabolism and stress resistance. Salt stress is one of the major stresses faced by rice. However, there is a lack of systematic understanding of the BURP family involved in the regulation of salt stress in rice, especially for the gene OsBURP12. Our study used japonica rice variety Zhonghua 11 (ZH11), as the wild type (WT), and its OsBURP12 knockout lines to fill the gap of the understanding. NaCl solution was used for salt stress treatment. Related physiological and biochemical analyses were carried out to investigate the regulatory mechanism of OsBURP12 on salt tolerance in rice seedlings. The results showed that the survival rate, seedling height, root length and fresh weight of the knockout lines were significantly higher than those of the WT under salt stress, indicating that the salt tolerance of the knockout lines was significantly better than that of the WT. Under salt stress, the knockout lines had significantly higher antioxidant enzyme activity, lower accumulation of ROS (reactive oxygen species) and higher expression of genes related to ROS-scavenging than the WT. In addition, the knockout lines showed significantly lower PG activity and Na+ content, but significantly higher pectin content, K+ and Ca2+ content than the WT. Furthermore, the knockout lines had significantly higher ABA and ACC levels than the WT. Quantitative PCR analysis showed that the expression levels of genes related to ABA and ethylene synthesis and signaling were significantly higher in the knockout lines than in the WT. The results suggested that the knockout of OsBURP12 can improve ROS-scavenging ability, affect PG activity, regulate Na+ uptake, and mediate the synthesis and metabolism of ABA and ACC in response to salt stress for a tolerance in rice. Our study laid the foundation for further analysis of the function of BURP family genes in plants, and provided a valuable genetic resource for studying the mechanism of salt tolerance in rice.
{"title":"Knockout of OsBURP12 enhances salt tolerance in rice seedlings","authors":"Zengtong Luo ,&nbsp;Sijia Yu ,&nbsp;Jialing Chen ,&nbsp;Qianyi Liu ,&nbsp;Mangu Hu ,&nbsp;Xiao Yang ,&nbsp;Yongxiang Huang ,&nbsp;Wuming Xiao","doi":"10.1016/j.envexpbot.2025.106097","DOIUrl":"10.1016/j.envexpbot.2025.106097","url":null,"abstract":"<div><div>The BURP gene family encodes the BURP domain protein, a type of plant-specific protein that plays an important role in plant development, metabolism and stress resistance. Salt stress is one of the major stresses faced by rice. However, there is a lack of systematic understanding of the BURP family involved in the regulation of salt stress in rice, especially for the gene <em>OsBURP12</em>. Our study used <em>japonica</em> rice variety Zhonghua 11 (ZH11), as the wild type (WT), and its <em>OsBURP12</em> knockout lines to fill the gap of the understanding. NaCl solution was used for salt stress treatment. Related physiological and biochemical analyses were carried out to investigate the regulatory mechanism of <em>OsBURP12</em> on salt tolerance in rice seedlings. The results showed that the survival rate, seedling height, root length and fresh weight of the knockout lines were significantly higher than those of the WT under salt stress, indicating that the salt tolerance of the knockout lines was significantly better than that of the WT. Under salt stress, the knockout lines had significantly higher antioxidant enzyme activity, lower accumulation of ROS (reactive oxygen species) and higher expression of genes related to ROS-scavenging than the WT. In addition, the knockout lines showed significantly lower PG activity and Na<sup>+</sup> content, but significantly higher pectin content, K<sup>+</sup> and Ca<sup>2+</sup> content than the WT. Furthermore, the knockout lines had significantly higher ABA and ACC levels than the WT. Quantitative PCR analysis showed that the expression levels of genes related to ABA and ethylene synthesis and signaling were significantly higher in the knockout lines than in the WT. The results suggested that the knockout of <em>OsBURP12</em> can improve ROS-scavenging ability, affect PG activity, regulate Na<sup>+</sup> uptake, and mediate the synthesis and metabolism of ABA and ACC in response to salt stress for a tolerance in rice. Our study laid the foundation for further analysis of the function of BURP family genes in plants, and provided a valuable genetic resource for studying the mechanism of salt tolerance in rice.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"231 ","pages":"Article 106097"},"PeriodicalIF":4.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elevated nitrogen supply enhances the recovery capability of alfalfa following rewatering by regulating carbon allocation
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2025-01-30 DOI: 10.1016/j.envexpbot.2025.106095
Luanzi Sun , Kun Zhou , Jiacun Guo , Junrui Zang , Shipeng Liu
Drought events are increasingly frequent, posing a significant threat to plant growth and survival. Nitrogen (N) has been shown to improve drought tolerance in plants, but its role in facilitating recovery growth following rewatering after drought stress remains poorly understood. The alfalfa cultivated under hydroponic conditions with varying levels of N (Low N: 1 mM; Medium N: 4 mM; High: 7 mM) was subjected to drought-rewatering which was simulated by the addition and subsequent removal of PEG from the nutrient solution. The allocation of biomass (R/S) and non-structural carbohydrates (NSC) between shoots and roots, and the percentage of assimilation allocated to NSC were investigated at the end of the drought, early stage (7 days post-rewatering), and late stage of rewatering (35 days post-rewatering). The results revealed that after 35 days of rewatering, the alfalfa grown under medium N level had fully recovered its growth to that of their well-watered control groups, with no significant difference in total dry weights; nevertheless, those grown under low and high N levels had total dry weights that were 18.3 % and 18.8 % lower than those of their corresponding control groups, respectively. Following rewatering, similar to the drought period, plants exposed to higher N levels tended to allocate more biomass and NSC toward roots rather than shoots. At 35 days after rewatering, the R/S of plants under the low N supply level decreased by 9.6 %; that of plants under the medium N supply did not change significantly; whereas that of plants under the high N supply increased by 46.6 % in comparison with their corresponding control group. Furthermore, a higher N supply level facilitated carbon allocation for tissue growth rather than reserving NSC, similar to the effect of N supply during the drought period. Therefore, a higher N supply may enhance the recovery capability of the plant after rewatering but may delay the recovery rate. During various stages of the drought-rewatering process, increasing the N supply level influenced the allocation of biomass and NSC between shoots and roots through different key enzymes and sucrose transporters. Our study provides valuable insights into the carbon regulatory mechanisms utilized by plants in response to rewatering after drought under varying N supply conditions. This contributes to a deeper understanding of plant adaptation strategies in the face of drought events.
{"title":"Elevated nitrogen supply enhances the recovery capability of alfalfa following rewatering by regulating carbon allocation","authors":"Luanzi Sun ,&nbsp;Kun Zhou ,&nbsp;Jiacun Guo ,&nbsp;Junrui Zang ,&nbsp;Shipeng Liu","doi":"10.1016/j.envexpbot.2025.106095","DOIUrl":"10.1016/j.envexpbot.2025.106095","url":null,"abstract":"<div><div>Drought events are increasingly frequent, posing a significant threat to plant growth and survival. Nitrogen (N) has been shown to improve drought tolerance in plants, but its role in facilitating recovery growth following rewatering after drought stress remains poorly understood. The alfalfa cultivated under hydroponic conditions with varying levels of N (Low N: 1 mM; Medium N: 4 mM; High: 7 mM) was subjected to drought-rewatering which was simulated by the addition and subsequent removal of PEG from the nutrient solution. The allocation of biomass (R/S) and non-structural carbohydrates (NSC) between shoots and roots, and the percentage of assimilation allocated to NSC were investigated at the end of the drought, early stage (7 days post-rewatering), and late stage of rewatering (35 days post-rewatering). The results revealed that after 35 days of rewatering, the alfalfa grown under medium N level had fully recovered its growth to that of their well-watered control groups, with no significant difference in total dry weights; nevertheless, those grown under low and high N levels had total dry weights that were 18.3 % and 18.8 % lower than those of their corresponding control groups, respectively. Following rewatering, similar to the drought period, plants exposed to higher N levels tended to allocate more biomass and NSC toward roots rather than shoots. At 35 days after rewatering, the R/S of plants under the low N supply level decreased by 9.6 %; that of plants under the medium N supply did not change significantly; whereas that of plants under the high N supply increased by 46.6 % in comparison with their corresponding control group. Furthermore, a higher N supply level facilitated carbon allocation for tissue growth rather than reserving NSC, similar to the effect of N supply during the drought period. Therefore, a higher N supply may enhance the recovery capability of the plant after rewatering but may delay the recovery rate. During various stages of the drought-rewatering process, increasing the N supply level influenced the allocation of biomass and NSC between shoots and roots through different key enzymes and sucrose transporters. Our study provides valuable insights into the carbon regulatory mechanisms utilized by plants in response to rewatering after drought under varying N supply conditions. This contributes to a deeper understanding of plant adaptation strategies in the face of drought events.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"231 ","pages":"Article 106095"},"PeriodicalIF":4.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomic insights into the stress signaling and drought tolerance mechanisms in sea-island cotton (Gossypium barbadense) 海岛棉(Gossypium barbadense)胁迫信号和抗旱机制的转录组学研究
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-12-01 DOI: 10.1016/j.envexpbot.2024.106048
Tahir Mahmood , Shoupu He , De Zhu , Hongge Li , Xiaoli Geng , Baojun Chen , Xianpeng Xiong , Xuai Dai , Xiongfeng Ma , Xiongming Du , Guanjing Hu
Drought stress significantly impacts plant growth and agricultural productivity. Elucidating the molecular mechanisms underlying drought stress response and plant tolerance is crucial for developing resilient crops. In Gossypium barbadense (G. barbadense), the specific genetic responses to drought stress remain underexplored. To provide insights into the transcriptomic dynamics and tolerance mechanisms in G. barbadense, we screened a diverse panel of G. barbadense accessions to identify drought-tolerant genotypes and investigate drought-stress responses across root and shoot tissues at two distinct time points. Differentially expressed genes (DEGs) analysis revealed diverse drought-responsive genes across tissue types and treatment time points. Functional enrichment and predictive protein-protein interaction (PPI) network analyses elucidated intricate patterns of drought-stress signaling pathways and transcriptional regulatory mechanisms. These upregulated DEGs were enriched in functional categories such as hormone signal transduction, phosphatidylinositol signaling system, ubiquitin-mediated proteolysis, phenylpropanoid biosynthesis, glutathione metabolism, and carbon metabolism pathways. The PPI network analysis underscores the activation of key signaling genes such as plant U-box E3 ubiquitin ligases (PUBs), protein phosphatase 2 C (PP2Cs), and F-Box genes, as well as transcriptional factors (CBF/NFYA) and various effector genes. These networks revealed the activation of effector genes involved in phenylpropanoid biosynthesis (Thioredoxin like 2–1, 1-Cys), glutathione metabolism (Thioredoxin, GPX6), and carbohydrate/sugar metabolism (GBSSI, AMY1.1). Gene silencing experiments validated the regulatory roles predicted for PUBs and PP2Cs in stress signaling and NFYA transcriptional factor in modifying the plant morphology and physiology to enhance drought tolerance. This research provides critical insights into the genetic signatures of stress signaling and regulatory pathways associated with drought tolerance in G. barbadense. The identified candidate genes are valuable for targeted breeding efforts to enhance drought tolerance and crop yield.
干旱胁迫显著影响植物生长和农业生产力。阐明干旱胁迫响应和植物耐受性的分子机制对培育抗旱性作物至关重要。在巴巴多斯棉(G. barbadense)中,对干旱胁迫的特定遗传反应尚未得到充分的研究。为了深入了解巴巴多斯(G. barbadense)的转录组动力学和耐旱机制,我们筛选了不同种类的巴巴多斯(G. barbadense)材料,以鉴定耐旱基因型,并研究了两个不同时间点根系和茎部组织对干旱胁迫的响应。差异表达基因(DEGs)分析显示,不同组织类型和处理时间点的干旱响应基因存在差异。功能富集和预测蛋白-蛋白相互作用(PPI)网络分析揭示了干旱胁迫信号通路的复杂模式和转录调控机制。这些上调的deg在激素信号转导、磷脂酰肌醇信号系统、泛素介导的蛋白质水解、苯丙素生物合成、谷胱甘肽代谢和碳代谢途径等功能类别中富集。PPI网络分析强调了关键信号基因的激活,如植物U-box E3泛素连接酶(pub)、蛋白磷酸酶2 C (pp2c)和F-Box基因,以及转录因子(CBF/NFYA)和各种效应基因。这些网络揭示了参与苯丙素生物合成(Thioredoxin like 2 - 1,1 - cys)、谷胱甘肽代谢(Thioredoxin, GPX6)和碳水化合物/糖代谢(GBSSI, AMY1.1)的效应基因的激活。基因沉默实验验证了预测的pub和pp2c在胁迫信号中的调控作用以及NFYA转录因子在改变植物形态和生理以增强耐旱性方面的调控作用。这项研究为巴贝登斯干旱耐受性相关的胁迫信号和调控途径的遗传特征提供了重要的见解。所鉴定的候选基因对提高作物抗旱性和产量的有针对性育种工作具有重要价值。
{"title":"Transcriptomic insights into the stress signaling and drought tolerance mechanisms in sea-island cotton (Gossypium barbadense)","authors":"Tahir Mahmood ,&nbsp;Shoupu He ,&nbsp;De Zhu ,&nbsp;Hongge Li ,&nbsp;Xiaoli Geng ,&nbsp;Baojun Chen ,&nbsp;Xianpeng Xiong ,&nbsp;Xuai Dai ,&nbsp;Xiongfeng Ma ,&nbsp;Xiongming Du ,&nbsp;Guanjing Hu","doi":"10.1016/j.envexpbot.2024.106048","DOIUrl":"10.1016/j.envexpbot.2024.106048","url":null,"abstract":"<div><div>Drought stress significantly impacts plant growth and agricultural productivity. Elucidating the molecular mechanisms underlying drought stress response and plant tolerance is crucial for developing resilient crops. In <em>Gossypium barbadense</em> (<em>G. barbadense</em>), the specific genetic responses to drought stress remain underexplored. To provide insights into the transcriptomic dynamics and tolerance mechanisms in <em>G. barbadense</em>, we screened a diverse panel of <em>G. barbadense</em> accessions to identify drought-tolerant genotypes and investigate drought-stress responses across root and shoot tissues at two distinct time points. Differentially expressed genes (DEGs) analysis revealed diverse drought-responsive genes across tissue types and treatment time points. Functional enrichment and predictive protein-protein interaction (PPI) network analyses elucidated intricate patterns of drought-stress signaling pathways and transcriptional regulatory mechanisms. These upregulated DEGs were enriched in functional categories such as hormone signal transduction, phosphatidylinositol signaling system, ubiquitin-mediated proteolysis, phenylpropanoid biosynthesis, glutathione metabolism, and carbon metabolism pathways. The PPI network analysis underscores the activation of key signaling genes such as plant U-box E3 ubiquitin ligases (PUBs), protein phosphatase 2 C (PP2Cs), and F-Box genes, as well as transcriptional factors (CBF/NFYA) and various effector genes. These networks revealed the activation of effector genes involved in phenylpropanoid biosynthesis (<em>Thioredoxin like 2–1, 1-Cys</em>), glutathione metabolism (<em>Thioredoxin, GPX6</em>), and carbohydrate/sugar metabolism (<em>GBSSI, AMY1.1</em>). Gene silencing experiments validated the regulatory roles predicted for PUBs and PP2Cs in stress signaling and <em>NFYA</em> transcriptional factor in modifying the plant morphology and physiology to enhance drought tolerance. This research provides critical insights into the genetic signatures of stress signaling and regulatory pathways associated with drought tolerance in <em>G. barbadense</em>. The identified candidate genes are valuable for targeted breeding efforts to enhance drought tolerance and crop yield.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106048"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exogenous γ-aminobutyric acid (GABA) provides a carbon skeleton to promote the accumulation of sugar and unsaturated fatty acids in vegetable soybean seeds 外源γ-氨基丁酸(GABA)为菜用大豆种子提供碳骨架,促进糖和不饱和脂肪酸的积累
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-11-24 DOI: 10.1016/j.envexpbot.2024.106052
Fengqiong Chen , Yating Wang , Yiyang Liu , Qiusen Chen , Hanlin Liu , Jin Tian , Mengxue Wang , Chunyuan Ren , Qiang Zhao , Fengjun Yang , Jinpeng Wei , Gaobo Yu , Yuxian Zhang
γ-aminobutyric acid (GABA) influences various physiological processes in plants, particularly in carbon and nitrogen metabolism. However, the mechanism underlying carbon (sucrose and unsaturated fatty acid) metabolism in vegetable soybeans was still unknown. In this study, a foliar spray of GABA (10 mM) elevated the level of Ca2+ by up-regulating the expression of calmodulin (GmCaM), which increased glutamate decarboxylase (GAD) activity and boosted endogenous GABA content. This, in turn, enhanced the expression of coding genes of GABA transferase (GmGABA-T) and succinic semialdehyde dehydrogenase (GmSSADH), as well as the activity of GABA transferase (GABA-T), activated the GABA shunt to supply carbon to the tricarboxylic acid (TCA) cycle, thus improved carbon metabolism. The gene expression and activity of sucrose metabolism-related enzymes were also enhanced, leading to the increased accumulation of total soluble sugars, sucrose, glucose, etc. Additionally, exogenous GABA treatment elevated the level of unsaturated fatty acids, including omega-3 arachidonic acid, linoleic acid, alpha-linolenic acid, etc. However, these effects were attenuated by 3-mercaplopropionic acid (3-MP), an inhibitor of GABA synthesis. In summary, exogenous GABA provides a carbon skeleton that promotes the accumulation of sugar and unsaturated fatty acids in vegetable soybean seeds. This research provides a valuable theory for further improving the yield and quality of vegetable soybeans.
γ-氨基丁酸(GABA)影响植物的多种生理过程,特别是碳氮代谢。然而,菜用大豆中碳(蔗糖和不饱和脂肪酸)代谢的机制尚不清楚。在这项研究中,叶面喷施GABA(10 mM)通过上调钙调素(GmCaM)的表达来提高Ca2+水平,从而增加谷氨酸脱羧酶(GAD)活性,提高内源性GABA含量。从而增强GABA转移酶(GmGABA-T)和丁二酸半醛脱氢酶(GmSSADH)编码基因的表达以及GABA转移酶(GABA- t)的活性,激活GABA分流向三羧酸(TCA)循环供碳,从而改善碳代谢。蔗糖代谢相关酶的基因表达和活性也增强,导致总可溶性糖、蔗糖、葡萄糖等的积累增加。此外,外源GABA处理提高了不饱和脂肪酸的水平,包括omega-3花生四烯酸、亚油酸、α -亚麻酸等。然而,这些作用被3-巯基丙酸(3-MP)(一种GABA合成抑制剂)减弱。综上所述,外源GABA提供了一个碳骨架,促进了蔬菜大豆种子中糖和不饱和脂肪酸的积累。本研究为进一步提高菜用大豆的产量和品质提供了有价值的理论依据。
{"title":"Exogenous γ-aminobutyric acid (GABA) provides a carbon skeleton to promote the accumulation of sugar and unsaturated fatty acids in vegetable soybean seeds","authors":"Fengqiong Chen ,&nbsp;Yating Wang ,&nbsp;Yiyang Liu ,&nbsp;Qiusen Chen ,&nbsp;Hanlin Liu ,&nbsp;Jin Tian ,&nbsp;Mengxue Wang ,&nbsp;Chunyuan Ren ,&nbsp;Qiang Zhao ,&nbsp;Fengjun Yang ,&nbsp;Jinpeng Wei ,&nbsp;Gaobo Yu ,&nbsp;Yuxian Zhang","doi":"10.1016/j.envexpbot.2024.106052","DOIUrl":"10.1016/j.envexpbot.2024.106052","url":null,"abstract":"<div><div>γ-aminobutyric acid (GABA) influences various physiological processes in plants, particularly in carbon and nitrogen metabolism. However, the mechanism underlying carbon (sucrose and unsaturated fatty acid) metabolism in vegetable soybeans was still unknown. In this study, a foliar spray of GABA (10 mM) elevated the level of Ca<sup>2+</sup> by up-regulating the expression of calmodulin (<em>GmCaM</em>), which increased glutamate decarboxylase (GAD) activity and boosted endogenous GABA content. This, in turn, enhanced the expression of coding genes of GABA transferase (<em>GmGABA-T</em>) and succinic semialdehyde dehydrogenase (<em>GmSSADH</em>), as well as the activity of GABA transferase (GABA-T), activated the GABA shunt to supply carbon to the tricarboxylic acid (TCA) cycle, thus improved carbon metabolism. The gene expression and activity of sucrose metabolism-related enzymes were also enhanced, leading to the increased accumulation of total soluble sugars, sucrose, glucose, etc. Additionally, exogenous GABA treatment elevated the level of unsaturated fatty acids, including omega-3 arachidonic acid, linoleic acid, alpha-linolenic acid, etc. However, these effects were attenuated by 3-mercaplopropionic acid (3-MP), an inhibitor of GABA synthesis. In summary, exogenous GABA provides a carbon skeleton that promotes the accumulation of sugar and unsaturated fatty acids in vegetable soybean seeds. This research provides a valuable theory for further improving the yield and quality of vegetable soybeans.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106052"},"PeriodicalIF":4.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RhbHLH92 positively regulates the dehydration tolerance by interacting with RhMYB123 in rose petals (Rosa hybrida) RhbHLH92 通过与玫瑰花瓣中的 RhMYB123 相互作用正向调节脱水耐受性
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-11-23 DOI: 10.1016/j.envexpbot.2024.106049
Ping Luo , Huanyu Zhang , Yeni Chen , Yongyi Cui , Wen Chen
Drought increasingly constitutes a significant constraint that detrimentally affects plant growth and the productivity of agricultural crops. The bHLHs is pivotal in enabling plants to withstand various abiotic stresses. However, the specific roles of bHLHs in stress remain limited. Here, we explore the role of RhbHLH92 from the Rosa hybrida according to the previous RNA-seq data. The expression of RhbHLH92 was enhanced under several abiotic stress conditions, especially dehydration. RhbHLH92 is located in the nucleus. Enhanced dehydration and drought tolerance were observed in tobacco and rose petals overexpressing RhbHLH92. These genetically modified plants maintained better water balance, showed decreased levels of reactive oxygen species, and exhibited elevated activity of antioxidant enzymes along with increased expression of drought resistance genes compared to WT. Conversely, suppression of RhbHLH92 in rose petals using virus-induced gene silencing (VIGS) heightened their vulnerability to dehydration and reduced the expression of genes associated with stress tolerance. Yeast two-hybrid and BiFC confirmed that RhbHLH92 physically interacts with RhMYB123, a R2R3-type TF. RhMYB123 overexpression in rose petals similarly boosted dehydration tolerance. RhbHLH92 and RhMYB123 could directly bind to the Δ-1-pyrroline-5-carboxylate synthetase (RhP5CS) promoter, the RhbHLH92-RhMYB123 complex led to higher transcript levels of RhP5CS. These findings elucidate a new pathway through which RhbHLH92 enhances drought tolerance in roses, offering potential strategies for the development of drought-resistant crop varieties.
干旱日益成为影响植物生长和农作物产量的重要制约因素。bHLHs 在植物抵御各种非生物胁迫方面起着关键作用。然而,bHLHs 在胁迫中的具体作用仍然有限。在此,我们根据之前的 RNA-seq 数据探讨了蔷薇杂交种中 RhbHLH92 的作用。在多种非生物胁迫条件下,尤其是脱水条件下,RhbHLH92的表达增强。RhbHLH92 位于细胞核中。在过表达 RhbHLH92 的烟草和玫瑰花瓣中观察到了更强的脱水和抗旱能力。与 WT 相比,这些转基因植物能保持更好的水分平衡,活性氧水平降低,抗氧化酶活性提高,抗旱基因表达增加。相反,利用病毒诱导基因沉默(VIGS)技术抑制玫瑰花瓣中的 RhbHLH92 会增加其对脱水的脆弱性,并降低与抗逆性相关的基因的表达。酵母双杂交和 BiFC 证实 RhbHLH92 与 R2R3 型 TF RhMYB123 有物理相互作用。RhMYB123在玫瑰花瓣中的过表达同样提高了耐脱水性。RhbHLH92和RhMYB123可直接与Δ-1-吡咯啉-5-羧酸合成酶(RhP5CS)启动子结合,RhbHLH92-RhMYB123复合物可提高RhP5CS的转录水平。这些发现阐明了 RhbHLH92 提高玫瑰耐旱性的新途径,为抗旱作物品种的开发提供了潜在的策略。
{"title":"RhbHLH92 positively regulates the dehydration tolerance by interacting with RhMYB123 in rose petals (Rosa hybrida)","authors":"Ping Luo ,&nbsp;Huanyu Zhang ,&nbsp;Yeni Chen ,&nbsp;Yongyi Cui ,&nbsp;Wen Chen","doi":"10.1016/j.envexpbot.2024.106049","DOIUrl":"10.1016/j.envexpbot.2024.106049","url":null,"abstract":"<div><div>Drought increasingly constitutes a significant constraint that detrimentally affects plant growth and the productivity of agricultural crops. The bHLHs is pivotal in enabling plants to withstand various abiotic stresses. However, the specific roles of bHLHs in stress remain limited. Here, we explore the role of <em>RhbHLH92</em> from the <em>Rosa hybrida</em> according to the previous RNA-seq data. The expression of <em>RhbHLH92</em> was enhanced under several abiotic stress conditions, especially dehydration. RhbHLH92 is located in the nucleus. Enhanced dehydration and drought tolerance were observed in tobacco and rose petals overexpressing <em>RhbHLH92.</em> These genetically modified plants maintained better water balance, showed decreased levels of reactive oxygen species, and exhibited elevated activity of antioxidant enzymes along with increased expression of drought resistance genes compared to WT. Conversely, suppression of <em>RhbHLH92</em> in rose petals using virus-induced gene silencing (VIGS) heightened their vulnerability to dehydration and reduced the expression of genes associated with stress tolerance. Yeast two-hybrid and BiFC confirmed that RhbHLH92 physically interacts with RhMYB123, a R2R3-type TF. <em>RhMYB123</em> overexpression in rose petals similarly boosted dehydration tolerance. RhbHLH92 and RhMYB123 could directly bind to the <em>Δ-1-pyrroline-5-carboxylate synthetase</em> (<em>RhP5CS</em>) promoter, the RhbHLH92-RhMYB123 complex led to higher transcript levels of <em>RhP5CS</em>. These findings elucidate a new pathway through which <em>RhbHLH92</em> enhances drought tolerance in roses, offering potential strategies for the development of drought-resistant crop varieties.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106049"},"PeriodicalIF":4.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142720402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PagbZIP75 decreases the ROS accumulation to enhance salt tolerance of poplar via the ABA signaling PagbZIP75 通过 ABA 信号减少 ROS 积累,从而提高杨树的耐盐性
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-11-22 DOI: 10.1016/j.envexpbot.2024.106051
Jia Hu , Siyuan Nan , Lieding Zhou , Changhong Yu , Yajing Li , Kai Zhao , Shuhui Du , Youzhi Han , Shengji Wang
Poplar (Populus L.) is a fast-growing economic timber plant that is susceptible to salt stress. Here, PagbZIP75 (Potri.014G120800), which was isolated from 84 K poplar and upregulated in response to salt treatment, was investigated by generating overexpression (OE) and repression (RNAi) transgenic lines to elucidate its role in poplar salt stress tolerance through molecular and physiological approaches. PagbZIP75 localized in the nucleus and cell membrane but lacked transcriptional activation activity in yeast cells. Expression pattern analysis revealed that PagbZIP75 was induced by salt stress, peaking at 12 hours in roots and stems and 24 hours in leaves. Under salt stress, OE exhibited enhanced growth and a more robust root system compared to non-transgenic 84 K poplar (WT) and RNAi. DAB and NBT staining results demonstrated lower levels of reactive oxygen species (ROS) in OE leaves, alongwith reduced electrolyte leakage rate and superoxide anion (O2-) content, while the proline content and superoxide dismutase (SOD) activity were significantly elevated under salt stress. Based on the RNA-seq data, multilayered hierarchical gene regulatory network (ML-hGRN) around bZIP75 was illustrated and indicated that PagbZIP75 was induced by ABA hormone along with 10 salt-related co-expressed genes. Yeast one-hybrid (Y1H) experiments indicated the binding of PagAREB1 protein to the 0–208 bp upstream fragments of PagbZIP75, and dual luciferase assays (LUC) confirmed a negative interaction between AREB1 and bZIP75. Overall, this study provides a theoretical foundation for the enhancement of poplar salt tolerance by PagbZIP75 through the reduction of ROS accumulation via ABA signaling.
杨树(Populus L.)是一种快速生长的经济用材植物,易受盐胁迫影响。在此,研究人员通过产生过表达(OE)和抑制(RNAi)转基因品系,研究了从 84 K 杨树中分离并在盐处理中上调的 PagbZIP75(Potri.014G120800),以通过分子和生理方法阐明其在杨树耐盐胁迫中的作用。PagbZIP75 定位于细胞核和细胞膜,但在酵母细胞中缺乏转录激活活性。表达模式分析显示,PagbZIP75受到盐胁迫的诱导,在根和茎的12小时和叶的24小时达到峰值。在盐胁迫下,与非转基因 84 K 杨树(WT)和 RNAi 相比,OE 表现出更强的生长能力和更健壮的根系。DAB 和 NBT 染色结果表明,在盐胁迫下,OE 叶片中活性氧(ROS)水平降低,电解质渗漏率和超氧阴离子(O2-)含量减少,而脯氨酸含量和超氧化物歧化酶(SOD)活性显著升高。基于RNA-seq数据,绘制了围绕bZIP75的多层分级基因调控网络(ML-hGRN),表明PagbZIP75与10个与盐相关的共表达基因一起被ABA激素诱导。酵母单杂交(Y1H)实验表明,PagAREB1蛋白与PagbZIP75上游0-208 bp片段结合,双荧光素酶测定(LUC)证实了AREB1与bZIP75之间的负作用。总之,本研究为 PagbZIP75 通过 ABA 信号减少 ROS 积累增强杨树耐盐性提供了理论依据。
{"title":"PagbZIP75 decreases the ROS accumulation to enhance salt tolerance of poplar via the ABA signaling","authors":"Jia Hu ,&nbsp;Siyuan Nan ,&nbsp;Lieding Zhou ,&nbsp;Changhong Yu ,&nbsp;Yajing Li ,&nbsp;Kai Zhao ,&nbsp;Shuhui Du ,&nbsp;Youzhi Han ,&nbsp;Shengji Wang","doi":"10.1016/j.envexpbot.2024.106051","DOIUrl":"10.1016/j.envexpbot.2024.106051","url":null,"abstract":"<div><div>Poplar (<em>Populus</em> L.) is a fast-growing economic timber plant that is susceptible to salt stress. Here, <em>PagbZIP75</em> (<em>Potri.014G120800</em>), which was isolated from 84 K poplar and upregulated in response to salt treatment, was investigated by generating overexpression (OE) and repression (RNAi) transgenic lines to elucidate its role in poplar salt stress tolerance through molecular and physiological approaches. PagbZIP75 localized in the nucleus and cell membrane but lacked transcriptional activation activity in yeast cells. Expression pattern analysis revealed that <em>PagbZIP75</em> was induced by salt stress, peaking at 12 hours in roots and stems and 24 hours in leaves. Under salt stress, OE exhibited enhanced growth and a more robust root system compared to non-transgenic 84 K poplar (WT) and RNAi. DAB and NBT staining results demonstrated lower levels of reactive oxygen species (ROS) in OE leaves, alongwith reduced electrolyte leakage rate and superoxide anion (O<sub>2</sub><sup>-</sup>) content, while the proline content and superoxide dismutase (SOD) activity were significantly elevated under salt stress. Based on the RNA-seq data, multilayered hierarchical gene regulatory network (ML-hGRN) around <em>bZIP75</em> was illustrated and indicated that <em>PagbZIP75</em> was induced by ABA hormone along with 10 salt-related co-expressed genes. Yeast one-hybrid (Y1H) experiments indicated the binding of PagAREB1 protein to the 0–208 bp upstream fragments of <em>PagbZIP75</em>, and dual luciferase assays (LUC) confirmed a negative interaction between AREB1 and bZIP75. Overall, this study provides a theoretical foundation for the enhancement of poplar salt tolerance by <em>PagbZIP75</em> through the reduction of ROS accumulation via ABA signaling.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106051"},"PeriodicalIF":4.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142720401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Light-nutrient interaction orchestrates leaf dynamics, nitrogen assimilation, and cellular energetics in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze 光-营养相互作用协调叶片动力学、氮同化和细胞能量学。& C.A.Mey)。Kuntze
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-11-22 DOI: 10.1016/j.envexpbot.2024.106044
Khairul Azree Rosli , Azizah Misran , Latifah Saiful Yazan , Puteri Edaroyati Megat Wahab
Light and nutrients are vital environmental factors shaping plant growth and metabolism, yet their interactive effects on leaf dynamics, nitrogen assimilation, and cellular energetics remain largely unexplored. We aimed to investigate these processes in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze under two light levels; high-light (HL, 0 % shade) and low-light (LL, 50 % shade) combined with four nutrient levels; low (NPK1, 40 mg kg−1), moderate (NPK2, 80 mg kg−1), high (NPK3, 120 mg kg−1) and very high (NPK4, 160 mg kg−1). High-light conditions and high-nutrient levels (HL-NPK3) synergistically enhanced leaf mass area by 44 % with net photosynthesis rates and nitrate reductase activity increasing by up to 17.62 ± 0.89 µmol CO2 m−2 s−1 and 0.34 ± 0.02 μmol NO2 cm−2 h−1 each. Low-light and moderate-nutrient levels (LL-NPK2) triggered a 42 % increase in specific leaf area and threefold higher photosynthetic nitrogen use efficiency. Unexpectedly, high-light and moderate-nutrient levels (HL-NPK2) elicited peak vacuolar H+-ATPase and H+-pyrophosphatase activities at 15.6 % and 53.1 % each. This study also found significant positive correlations between chlorophyll content, nitrate reductase (r = 0.62, P < 0.01), and vacuolar H+-ATPase activity (r = 0.58, P < 0.01), suggesting a mechanism for maintaining high photosynthetic capacity and efficient nitrogen assimilation. The clustering of leaf area index, specific leaf area, and photosynthetic nitrogen use efficiency (similarity of > 70 %) suggests optimized leaf structure and nitrogen use in light-limited but nutrient-rich environments. Our findings show how A. rugosa adjusts its physiology in response to environmental conditions, with implications for understanding plant adaptation and improving cultivation practices.
光和养分是影响植物生长和代谢的重要环境因子,但它们对叶片动力学、氮同化和细胞能量学的相互作用仍未得到充分研究。我们的目的是研究这些过程的Agastache rugosa (Fisch)。,C.A.Mey)。昆策下两级光;高光照(HL, 0 %遮光)和低光照(LL, 50 %遮光)组合4种营养水平;低(NPK1, 40 mg kg−1),中等(NPK2, 80 mg kg−1),高(NPK3, 120 mg kg−1)和非常高(NPK4, 160 mg kg−1)。高光条件和高营养水平(HL-NPK3)协同提高叶片质量面积44 %,净光合速率和硝酸还原酶活性分别提高17.62±0.89 μmol CO2 m−2 s−1和0.34±0.02 μmol NO2 cm−2 h−1。低光照和中等营养水平(l - npk2)使比叶面积增加42% %,光合氮利用效率提高3倍。出乎意料的是,高光照和中等营养水平(HL-NPK2)诱导液泡H+- atp酶和H+-焦磷酸酶活性峰值分别为15.6% %和53.1 %。本研究还发现叶绿素含量与硝酸盐还原酶之间呈显著正相关(r = 0.62, P <;0.01),液泡H+- atp酶活性(r = 0.58, P <;0.01),表明其维持高光合能力和高效氮同化的机制。叶面积指数、比叶面积和光合氮利用效率的聚类(相似度);70 %)表明在光照受限但养分丰富的环境下,叶片结构和氮素利用得到优化。我们的研究结果揭示了苦荬菜如何根据环境条件调整其生理机能,这对理解植物适应性和改进栽培方法具有重要意义。
{"title":"Light-nutrient interaction orchestrates leaf dynamics, nitrogen assimilation, and cellular energetics in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze","authors":"Khairul Azree Rosli ,&nbsp;Azizah Misran ,&nbsp;Latifah Saiful Yazan ,&nbsp;Puteri Edaroyati Megat Wahab","doi":"10.1016/j.envexpbot.2024.106044","DOIUrl":"10.1016/j.envexpbot.2024.106044","url":null,"abstract":"<div><div>Light and nutrients are vital environmental factors shaping plant growth and metabolism, yet their interactive effects on leaf dynamics, nitrogen assimilation, and cellular energetics remain largely unexplored. We aimed to investigate these processes in <em>Agastache rugosa</em> (Fisch. &amp; C.A.Mey.) Kuntze under two light levels; high-light (HL, 0 % shade) and low-light (LL, 50 % shade) combined with four nutrient levels; low (NPK1, 40 mg kg<sup>−1</sup>), moderate (NPK2, 80 mg kg<sup>−1</sup>), high (NPK3, 120 mg kg<sup>−1</sup>) and very high (NPK4, 160 mg kg<sup>−1</sup>). High-light conditions and high-nutrient levels (HL-NPK3) synergistically enhanced leaf mass area by 44 % with net photosynthesis rates and nitrate reductase activity increasing by up to 17.62 ± 0.89 µmol CO<sub>2</sub> m<sup>−2</sup> s<sup>−1</sup> and 0.34 ± 0.02 μmol NO<sub>2</sub> cm<sup>−2</sup> h<sup>−1</sup> each. Low-light and moderate-nutrient levels (LL-NPK2) triggered a 42 % increase in specific leaf area and threefold higher photosynthetic nitrogen use efficiency. Unexpectedly, high-light and moderate-nutrient levels (HL-NPK2) elicited peak vacuolar H<sup>+</sup>-ATPase and H<sup>+</sup>-pyrophosphatase activities at 15.6 % and 53.1 % each. This study also found significant positive correlations between chlorophyll content, nitrate reductase (r = 0.62, P &lt; 0.01), and vacuolar H<sup>+</sup>-ATPase activity (r = 0.58, P &lt; 0.01), suggesting a mechanism for maintaining high photosynthetic capacity and efficient nitrogen assimilation. The clustering of leaf area index, specific leaf area, and photosynthetic nitrogen use efficiency (similarity of &gt; 70 %) suggests optimized leaf structure and nitrogen use in light-limited but nutrient-rich environments. Our findings show how <em>A</em>. <em>rugosa</em> adjusts its physiology in response to environmental conditions, with implications for understanding plant adaptation and improving cultivation practices.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106044"},"PeriodicalIF":4.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rocky soils alter the diurnal photosynthetic behavior of xerophytic species by regulating hydraulic properties 岩石土壤通过调节水力特性改变旱生植物的昼夜光合作用行为
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-11-19 DOI: 10.1016/j.envexpbot.2024.106045
Xiulong Zhang , Fanglan Li , Lulu Xie , Weikai Bao
Functional relationships between photosynthetic behavior and hydraulic properties are essential to characterize plant drought adaptation strategies. However, little is known about such relationships in response to varying rock fragment content (RFC), which could induce severe soil water deficit. We evaluated the leaf hydraulic properties and the timing of diurnal depression of photosynthesis in three xerophytic shrub species grown under different RFC levels (0, 25, 50, 75 % v v−1). We found that studied species grown in 25 % RFC soil conditions had higher leaf hydraulic conductance (Kleaf) and reached maximum photosynthetic rate (Amax) in the morning, while those grown in 75 % RFC soil conditions had lower Kleaf, but reached their Amax in the afternoon. In addition, species in 75 % RFC soil conditions also exhibited low leaf hydraulic vulnerability and narrow leaf hydraulic safety margins. Our results indicate that RFC modifies the diurnal gas exchange dynamics of xerophytic species by decreasing leaf hydraulic vulnerability and hydraulic safety margins. Specifically, species surviving in 75 % RFC soils are less vulnerable to drought induced water loss, and carbon assimilation depression were later than in 25 % or 0 % RFC soil conditions. However, when faced with severe drought, these species with latter CO2 uptake depression are at higher risk of hydraulic failure, because their safety margins are relatively narrow. Our results contribute to the knowledge of drought adaptation strategies in xerophytic species native to dry-hot rocky mountains.
光合作用行为与水力特性之间的功能关系对于确定植物的干旱适应策略至关重要。然而,人们对这种关系在不同岩石碎片含量(RFC)条件下的反应知之甚少,而岩石碎片含量的变化可能会导致严重的土壤缺水。我们评估了在不同 RFC 水平(0、25、50、75 % v-1)下生长的三个旱生灌木物种的叶片水力特性和光合作用昼夜抑制的时间。我们发现,在 25% RFC 土壤条件下生长的物种叶片水导(Kleaf)较高,光合速率(Amax)在上午达到最大值,而在 75% RFC 土壤条件下生长的物种叶片水导(Kleaf)较低,但光合速率(Amax)在下午达到最大值。此外,75% RFC 土壤条件下的物种还表现出较低的叶片水力脆弱性和较窄的叶片水力安全边际。我们的研究结果表明,RFC 通过降低叶片水力脆弱性和水力安全边际,改变了旱生植物的昼夜气体交换动力学。具体来说,与 25% 或 0% RFC 的土壤条件相比,生存在 75% RFC 土壤中的物种更不容易受到干旱引起的失水的影响,碳同化抑制也更晚。然而,当面临严重干旱时,这些二氧化碳吸收抑制较晚的物种发生水力衰竭的风险较高,因为它们的安全边际相对较窄。我们的研究结果有助于了解原产于干热岩山区的旱生植物的干旱适应策略。
{"title":"Rocky soils alter the diurnal photosynthetic behavior of xerophytic species by regulating hydraulic properties","authors":"Xiulong Zhang ,&nbsp;Fanglan Li ,&nbsp;Lulu Xie ,&nbsp;Weikai Bao","doi":"10.1016/j.envexpbot.2024.106045","DOIUrl":"10.1016/j.envexpbot.2024.106045","url":null,"abstract":"<div><div>Functional relationships between photosynthetic behavior and hydraulic properties are essential to characterize plant drought adaptation strategies. However, little is known about such relationships in response to varying rock fragment content (RFC), which could induce severe soil water deficit. We evaluated the leaf hydraulic properties and the timing of diurnal depression of photosynthesis in three xerophytic shrub species grown under different RFC levels (0, 25, 50, 75 % v v<sup>−1</sup>). We found that studied species grown in 25 % RFC soil conditions had higher leaf hydraulic conductance (<em>K</em><sub>leaf</sub>) and reached maximum photosynthetic rate (<em>A</em><sub>max</sub>) in the morning, while those grown in 75 % RFC soil conditions had lower <em>K</em><sub>leaf</sub>, but reached their <em>A</em><sub>max</sub> in the afternoon. In addition, species in 75 % RFC soil conditions also exhibited low leaf hydraulic vulnerability and narrow leaf hydraulic safety margins. Our results indicate that RFC modifies the diurnal gas exchange dynamics of xerophytic species by decreasing leaf hydraulic vulnerability and hydraulic safety margins. Specifically, species surviving in 75 % RFC soils are less vulnerable to drought induced water loss, and carbon assimilation depression were later than in 25 % or 0 % RFC soil conditions. However, when faced with severe drought, these species with latter CO<sub>2</sub> uptake depression are at higher risk of hydraulic failure, because their safety margins are relatively narrow. Our results contribute to the knowledge of drought adaptation strategies in xerophytic species native to dry-hot rocky mountains.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106045"},"PeriodicalIF":4.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physiological and biochemical changes induced by drought stress during the stem elongation and anthesis stages in the Triticum genus 干旱胁迫在小麦属植物茎伸长和开花期诱发的生理和生化变化
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-11-19 DOI: 10.1016/j.envexpbot.2024.106047
Sumitra Pantha , Benjamin Kilian , Hakan Özkan , Frederike Zeibig , Michael Frei
Drought stress negatively influences the growth, development, and grain yield of wheat by disrupting its morphological, physiological, and biochemical processes. This study examined the effects of drought stress during the stem elongation and anthesis developmental stages of species within the Triticum genus along with their drought adaptation mechanisms under fully watered and drought conditions. We tested the following two hypotheses: (1) drought tolerance mechanisms for osmotic and stomatal regulation that lead to oxidative stress are correlated between the stem elongation and anthesis stages and affect grain yield loss, and (2) compared with modern cultivars, wild wheat cultivars exhibit greater drought tolerance. To test these hypotheses, a greenhouse pot experiment was conducted using 17 genotypes of wild wheat relatives and landraces, with modern cultivars included for comparison. Drought stress was induced during the stem elongation and anthesis stages until the average soil moisture was approximately 15 % and 18 %, respectively, of the pot’s water holding capacity. The soil moisture was maintained at 80–90 % for the fully watered treatment. An examination of physiological and biochemical traits revealed that drought significantly reduced stomatal conductance (gsw) and relative water content (RWC) during both developmental stages. However, significant increases occurred in the malondialdehyde (MDA) content during both stages and in the proline content during the anthesis stage. Drought stress significantly decreased the number of days to heading and anthesis, indicating that drought escape occurs under severe drought stress. Furthermore, drought significantly decreased morphological and yield-related traits, with the greatest reduction (51 %) occurring in grain yield. Weakly significant positive associations of biochemical and some physiological traits between the stem elongation and anthesis stages partially confirmed our first hypothesis, whereas our results relating to the second hypothesis were inconclusive. We observed genotype-dependent responses to drought stress during both stages for various measured traits. No associations of RWC, proline, or MDA with grain yield were found. However, stomatal conductance was negatively correlated with grain yield under drought stress at the anthesis stage. Certain wild wheat genotypes and landraces exhibited drought avoidance, escape, and tolerance mechanisms, which positively contributed to grain yield. We identified T. monococcum subsp. sinskajae, T. boeoticum and T. dicoccoides as the most drought-tolerant genotypes. The findings of this study provide important insight for understanding the drought adaptation traits and their use in wheat breeding programs.
干旱胁迫会破坏小麦的形态、生理和生化过程,从而对其生长、发育和谷物产量产生负面影响。本研究考察了干旱胁迫对小麦茎伸长和开花发育阶段的影响,以及它们在充分浇水和干旱条件下的干旱适应机制。我们测试了以下两个假设:(1) 在茎伸长和开花阶段,导致氧化应激的渗透和气孔调节的耐旱机制是相关的,并影响谷物产量损失;(2) 与现代栽培品种相比,野生小麦栽培品种表现出更强的耐旱性。为了验证这些假设,我们利用 17 个野生小麦近缘种和陆地栽培种的基因型进行了温室盆栽实验,并将现代栽培种纳入其中进行比较。在茎伸长和开花阶段诱发干旱胁迫,直到平均土壤湿度分别约为花盆持水量的 15% 和 18%。充分浇水处理的土壤湿度保持在 80-90 %。对生理生化性状的研究表明,干旱会显著降低两个发育阶段的气孔导度(gsw)和相对含水量(RWC)。然而,两个阶段的丙二醛(MDA)含量和花期的脯氨酸含量都明显增加。干旱胁迫明显降低了打顶和开花的天数,表明在严重干旱胁迫下会出现旱逃现象。此外,干旱还显著降低了形态和产量相关性状,其中谷物产量的降幅最大(51%)。茎秆伸长和开花期之间的生化和一些生理性状之间存在微弱的正相关,部分证实了我们的第一个假设,而与第二个假设相关的结果尚无定论。在这两个阶段,我们观察到了基因型对各种测量性状的干旱胁迫反应。没有发现 RWC、脯氨酸或 MDA 与谷物产量相关。然而,在开花期干旱胁迫下,气孔导度与谷物产量呈负相关。某些野生小麦基因型和陆地品系表现出避旱、逃旱和耐旱机制,这对谷物产量有积极作用。我们发现 T. monococcum subsp. sinskajae、T. boeoticum 和 T. dicoccoides 是最耐旱的基因型。本研究的发现为了解干旱适应性状及其在小麦育种计划中的应用提供了重要启示。
{"title":"Physiological and biochemical changes induced by drought stress during the stem elongation and anthesis stages in the Triticum genus","authors":"Sumitra Pantha ,&nbsp;Benjamin Kilian ,&nbsp;Hakan Özkan ,&nbsp;Frederike Zeibig ,&nbsp;Michael Frei","doi":"10.1016/j.envexpbot.2024.106047","DOIUrl":"10.1016/j.envexpbot.2024.106047","url":null,"abstract":"<div><div>Drought stress negatively influences the growth, development, and grain yield of wheat by disrupting its morphological, physiological, and biochemical processes. This study examined the effects of drought stress during the stem elongation and anthesis developmental stages of species within the <em>Triticum</em> genus along with their drought adaptation mechanisms under fully watered and drought conditions. We tested the following two hypotheses: (1) drought tolerance mechanisms for osmotic and stomatal regulation that lead to oxidative stress are correlated between the stem elongation and anthesis stages and affect grain yield loss, and (2) compared with modern cultivars, wild wheat cultivars exhibit greater drought tolerance. To test these hypotheses, a greenhouse pot experiment was conducted using 17 genotypes of wild wheat relatives and landraces, with modern cultivars included for comparison. Drought stress was induced during the stem elongation and anthesis stages until the average soil moisture was approximately 15 % and 18 %, respectively, of the pot’s water holding capacity. The soil moisture was maintained at 80–90 % for the fully watered treatment. An examination of physiological and biochemical traits revealed that drought significantly reduced stomatal conductance (gsw) and relative water content (RWC) during both developmental stages. However, significant increases occurred in the malondialdehyde (MDA) content during both stages and in the proline content during the anthesis stage. Drought stress significantly decreased the number of days to heading and anthesis, indicating that drought escape occurs under severe drought stress. Furthermore, drought significantly decreased morphological and yield-related traits, with the greatest reduction (51 %) occurring in grain yield. Weakly significant positive associations of biochemical and some physiological traits between the stem elongation and anthesis stages partially confirmed our first hypothesis, whereas our results relating to the second hypothesis were inconclusive. We observed genotype-dependent responses to drought stress during both stages for various measured traits. No associations of RWC, proline, or MDA with grain yield were found. However, stomatal conductance was negatively correlated with grain yield under drought stress at the anthesis stage. Certain wild wheat genotypes and landraces exhibited drought avoidance, escape, and tolerance mechanisms, which positively contributed to grain yield. We identified <em>T. monococcum</em> subsp. <em>sinskajae</em>, <em>T. boeoticum</em> and <em>T. dicoccoides</em> as the most drought-tolerant genotypes. The findings of this study provide important insight for understanding the drought adaptation traits and their use in wheat breeding programs.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106047"},"PeriodicalIF":4.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analyses of the bHLH gene family in Populus trichocarpa reveal roles of four PtbHLHs in regulating the drought stress response 对毛白杨 bHLH 基因家族的分析揭示了四种 PtbHLH 在调控干旱胁迫响应中的作用
IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Pub Date : 2024-11-19 DOI: 10.1016/j.envexpbot.2024.106046
Haizhen Zhang , Shuhan Ye , Nan Wang, Ziping Xu, Shufang Gong
As one of the largest families of transcription factors in plants, the basic helix-loop-helix (bHLH) transcription factor family regulates a wide range of functions in plants. However, little is known about the functions of bHLH family members in Populus trichocarpa during plant growth and in the response to drought stress. In our study, 190 PtbHLH genes were identified in the P. trichocarpa genome and classified into 21 groups. Analyses of microarray datasets showed that most PtbHLH members not only have multiple functions in poplar growth, but also respond rapidly to drought stress in the leaves or roots. We selected four genes, PtbHLH35, PtbHLH121, PtbHLH137, and PtbHLH152, which were highly expressed in leaves or roots under drought stress, for functional validation analyses. These genes encoded nucleus-localized bHLH transcription factors. Transient expression of PtbHLH35, PtbHLH121, and PtbHLH152 in P. trichocarpa improved drought tolerance by activating the antioxidant system to eliminate reactive oxygen species and reduce the degree of cell damage in the leaves under drought stress. Overexpression of PtbHLH137 improved drought tolerance by activating antioxidant enzymes in the roots to eliminate reactive oxygen species, and by increasing the abscisic acid content in the roots in response to drought stress. Together, our findings provide insights into the functions of PtbHLH family members in growth and in the response to drought.
作为植物中最大的转录因子家族之一,碱性螺旋-环-螺旋(bHLH)转录因子家族调控着植物的多种功能。然而,人们对毛白杨(Populus trichocarpa)中 bHLH 家族成员在植物生长和干旱胁迫响应中的功能知之甚少。我们的研究在毛白杨基因组中鉴定了 190 个 PtbHLH 基因,并将其分为 21 组。微阵列数据集分析表明,大多数 PtbHLH 成员不仅在杨树生长过程中具有多种功能,而且还能在叶片或根部对干旱胁迫做出快速反应。我们选择了在干旱胁迫下在叶片或根部高表达的四个基因 PtbHLH35、PtbHLH121、PtbHLH137 和 PtbHLH152 进行功能验证分析。这些基因编码细胞核定位的 bHLH 转录因子。PtbHLH35、PtbHLH121和PtbHLH152在毛地黄中的瞬时表达能激活抗氧化系统,消除活性氧,降低干旱胁迫下叶片的细胞损伤程度,从而提高耐旱性。过表达 PtbHLH137 可激活根中的抗氧化酶以消除活性氧,并增加根中的赤霉酸含量以应对干旱胁迫,从而提高耐旱性。总之,我们的研究结果提供了有关 PtbHLH 家族成员在生长和干旱响应中的功能的见解。
{"title":"Analyses of the bHLH gene family in Populus trichocarpa reveal roles of four PtbHLHs in regulating the drought stress response","authors":"Haizhen Zhang ,&nbsp;Shuhan Ye ,&nbsp;Nan Wang,&nbsp;Ziping Xu,&nbsp;Shufang Gong","doi":"10.1016/j.envexpbot.2024.106046","DOIUrl":"10.1016/j.envexpbot.2024.106046","url":null,"abstract":"<div><div>As one of the largest families of transcription factors in plants, the basic helix-loop-helix (bHLH) transcription factor family regulates a wide range of functions in plants. However, little is known about the functions of bHLH family members in <em>Populus trichocarpa</em> during plant growth and in the response to drought stress. In our study, 190 <em>PtbHLH</em> genes were identified in the <em>P. trichocarpa</em> genome and classified into 21 groups. Analyses of microarray datasets showed that most <em>PtbHLH</em> members not only have multiple functions in poplar growth, but also respond rapidly to drought stress in the leaves or roots. We selected four genes, <em>PtbHLH35</em>, <em>PtbHLH121</em>, <em>PtbHLH137,</em> and <em>PtbHLH152,</em> which were highly expressed in leaves or roots under drought stress, for functional validation analyses. These genes encoded nucleus-localized bHLH transcription factors. Transient expression of <em>PtbHLH35</em>, <em>PtbHLH121,</em> and <em>PtbHLH152</em> in <em>P. trichocarpa</em> improved drought tolerance by activating the antioxidant system to eliminate reactive oxygen species and reduce the degree of cell damage in the leaves under drought stress. Overexpression of <em>PtbHLH137</em> improved drought tolerance by activating antioxidant enzymes in the roots to eliminate reactive oxygen species, and by increasing the abscisic acid content in the roots in response to drought stress. Together, our findings provide insights into the functions of <em>PtbHLH</em> family members in growth and in the response to drought.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106046"},"PeriodicalIF":4.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Environmental and Experimental Botany
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1