{"title":"Using plasma-activated water for decontamination of Salmonella spp. on common building surfaces in poultry houses","authors":"","doi":"10.1016/j.fm.2024.104673","DOIUrl":null,"url":null,"abstract":"<div><div>Plasma-activated water (PAW) has been shown to have antimicrobial properties, making it a promising tool for surface decontamination. This study evaluated the ability of PAW generated from high voltage atmospheric cold plasma to remove <em>Salmonella</em> from common surfaces (stainless steel (SS), polyvinyl chloride (PVC), concrete, and wood) found in poultry houses. PAW was generated by exposing distilled water to atmospheric cold plasma in 80% humid air at 90 kV and 60 Hz for 30 min. The resulting PAW contained 1120 ppm of nitrate and 1370 ppm of hydrogen peroxide, with a pH of 1.83. PAW was then applied to coupons of SS, PVC, wood, and concrete surfaces inoculated with 7–8 log<sub>10</sub> CFU of cocktail of <em>Salmonella</em> spp. (<em>S.</em> Typhimurium, <em>S</em>. Newport, <em>S.</em> Montevideo, and <em>S.</em> Enteritidis). PAW effectively reduced <em>Salmonella</em> levels on SS and PVC surfaces to below the detection limit within 30 s. On wood surfaces, a longer treatment time of 7.5 min was required to achieve a maximum reduction of 2.63 log<sub>10</sub> CFU, likely due to the porosity of the wood limiting PAW contact with the bacteria. On concrete surfaces, the reduction in Salmonella levels was only 0.98 log<sub>10</sub> CFU. This was likely due to the greater surface roughness and high alkalinity, which neutralized the PAW species.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0740002024002119","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Plasma-activated water (PAW) has been shown to have antimicrobial properties, making it a promising tool for surface decontamination. This study evaluated the ability of PAW generated from high voltage atmospheric cold plasma to remove Salmonella from common surfaces (stainless steel (SS), polyvinyl chloride (PVC), concrete, and wood) found in poultry houses. PAW was generated by exposing distilled water to atmospheric cold plasma in 80% humid air at 90 kV and 60 Hz for 30 min. The resulting PAW contained 1120 ppm of nitrate and 1370 ppm of hydrogen peroxide, with a pH of 1.83. PAW was then applied to coupons of SS, PVC, wood, and concrete surfaces inoculated with 7–8 log10 CFU of cocktail of Salmonella spp. (S. Typhimurium, S. Newport, S. Montevideo, and S. Enteritidis). PAW effectively reduced Salmonella levels on SS and PVC surfaces to below the detection limit within 30 s. On wood surfaces, a longer treatment time of 7.5 min was required to achieve a maximum reduction of 2.63 log10 CFU, likely due to the porosity of the wood limiting PAW contact with the bacteria. On concrete surfaces, the reduction in Salmonella levels was only 0.98 log10 CFU. This was likely due to the greater surface roughness and high alkalinity, which neutralized the PAW species.
期刊介绍:
Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.