Detection of carboxylesterases by an activatable NIR fluorescence probe with high selectivity in living systems

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2024-10-19 DOI:10.1016/j.jphotochem.2024.116088

Yu Wang , Ting Ma , Wenda Zhang

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Abstract

Carboxylesterases (CEs) have attracted increasingly attention in the physiological and pathological processes of many diseases such as diabetes, hepatocellular carcinoma (HCC) and drug metabolism. Selective detection of CEs activity is imperative to evaluate the pathophysiological process of CEs-related disease. Herein, a selective near-infrared (NIR) fluorescent probe (DCM-CE) with high selectivity is reported. DCM-CE was composed of dicyanoisophorone-based fluorophore and carbamate unit which is a highly selective identification group for CEs. DCM-CE exhibited good sensitivity for CEs detection at physiological pH and temperature. Furthermore, DCM-CE featured large Stokes shift (175 nm) and good at tracking the drug-induced trace change of CEs activity in HepG2 cells with little effect on cell viability. Moreover, DCM-CE was applied to monitor the activity of CEs in living mice. Taken all together, DCM-CE had extensive potential application value in detecting CEs for evaluating related diseases.

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在活体系统中利用具有高选择性的可激活近红外荧光探针检测羧基酯酶

羧基酯酶（CEs）在糖尿病、肝细胞癌（HCC）和药物代谢等多种疾病的生理和病理过程中日益受到关注。要评估 CEs 相关疾病的病理生理过程，必须对 CEs 活性进行选择性检测。本文报告了一种具有高选择性的近红外（NIR）荧光探针（DCM-CE）。DCM-CE 由基于二氰异佛尔酮的荧光团和氨基甲酸酯单元组成，氨基甲酸酯单元是 CEs 的高选择性识别基团。在生理 pH 值和温度条件下，DCM-CE 对 CEs 的检测具有良好的灵敏度。此外，DCM-CE 还具有较大的斯托克斯位移（175 nm），能很好地跟踪药物诱导的 HepG2 细胞中 CEs 活性的微量变化，且对细胞活力影响很小。此外，DCM-CE 还可用于监测活体小鼠体内 CEs 的活性。综上所述，DCM-CE 在检测 CEs 以评估相关疾病方面具有广泛的潜在应用价值。

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.