Removal mechanisms of pentachlorophenol in a horizontal-flow anaerobic immobilized biomass reactor (HAIB) inoculated with an indigenous estuarine sediment microbiota: adsorption and biodegradation processes

IF 3.1 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biodegradation Pub Date : 2024-10-29 DOI:10.1007/s10532-024-10096-z
Gunther Brucha, Alessandra Giordani, Bárbara Franco Vieira, Marcia Helena Rissato Zamariolli Damianovic, Flavia Talarico Saia, Leonardo Henrique Soares Damasceno, Johannes Gerson Janzen, Eugenio Foresti, Rosana Filomena Vazoller
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Abstract

Pentachlorophenol (PCP) is a highly toxic and carcinogenic compound with significant environmental impact, necessitating effective treatment technologies. This study evaluates PCP removal mechanisms, including adsorption and biodegradation, during the startup of a horizontal-flow anaerobic immobilized biomass reactor (HAIB), and examines the impact of PCP concentration on microbial diversity using denaturing gradient gel electrophoresis (DGGE). The primary mechanism for PCP removal in the HAIB was adsorption, effectively described by the Freundlich isotherm model. Adsorption efficiency ranged from 86 to 104% for PCP concentrations between 0.2 and 5.0 mg/L, and 46% to 64% for concentrations between 0.098 and 0.05 mg/L. Additionally, PCP degradation intermediates such as 2,3-DCP and 2,6-DCP were detected, indicating that biodegradation also occurred in the HAIB. Organic matter degradation averaged 81 ± 9%, and methane content in the biogas averaged 46 ± 9%, confirming the anaerobic process. No inhibition of microbial activity was observed due to PCP toxicity, even at a PCP load of 5 mg PCP/g STV per day. While the archaeal community showed only slight changes, with similarity coefficients ranging from 88 to 95%, the bacterial community was significantly affected by PCP, with similarity coefficients ranging from 18 to 50%. Bacterial groups were responsible for the initial PCP degradation, while the archaeal community was involved in metabolizing the resulting byproducts. The use of indigenous inoculum from the Santos-São Vicente estuary demonstrated its potential for effective PCP removal. Polyurethane foam proved to be an effective support material, enhancing the adsorption process and reducing PCP toxicity to the microbial consortium. This study provides valuable insights into PCP adsorption and biodegradation mechanisms in HAIB, highlighting the effectiveness of indigenous inoculum and polyurethane foam for PCP removal.

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在水平流厌氧固定生物质反应器(HAIB)中接种本地河口沉积物微生物群的五氯苯酚去除机制:吸附和生物降解过程
五氯苯酚(PCP)是一种剧毒致癌化合物,对环境有重大影响,因此需要有效的处理技术。本研究评估了水平流厌氧固定生物质反应器(HAIB)启动过程中的五氯苯酚去除机制,包括吸附和生物降解,并使用变性梯度凝胶电泳(DGGE)研究了五氯苯酚浓度对微生物多样性的影响。在 HAIB 中去除五氯苯酚的主要机制是吸附,弗伦德里希等温线模型对此进行了有效描述。五氯苯酚浓度在 0.2 至 5.0 毫克/升之间时,吸附效率为 86% 至 104%,浓度在 0.098 至 0.05 毫克/升之间时,吸附效率为 46% 至 64%。此外,还检测到 2,3-DCP 和 2,6-DCP 等五氯苯酚降解中间产物,表明 HAIB 中也存在生物降解。有机物降解率平均为 81 ± 9%,沼气中甲烷含量平均为 46 ± 9%,证实了厌氧过程。即使每天的五氯苯酚负荷为 5 毫克五氯苯酚/克 STV,也没有观察到五氯苯酚毒性对微生物活动的抑制。虽然古生物群落只发生了轻微的变化,相似系数从 88% 到 95% 不等,但细菌群落却受到五氯苯酚的显著影响,相似系数从 18% 到 50% 不等。细菌群落负责最初的五氯苯酚降解,而古细菌群落则参与代谢产生的副产品。使用桑托斯-圣维森特河口的本地接种物证明了其有效去除五氯苯酚的潜力。聚氨酯泡沫被证明是一种有效的支撑材料,可增强吸附过程并降低五氯苯酚对微生物群的毒性。这项研究为 HAIB 中的五氯苯酚吸附和生物降解机制提供了宝贵的见解,突出了本地接种物和聚氨酯泡沫对去除五氯苯酚的有效性。
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来源期刊
Biodegradation
Biodegradation 工程技术-生物工程与应用微生物
CiteScore
5.60
自引率
0.00%
发文量
36
审稿时长
6 months
期刊介绍: Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.
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