Exploring the inhibitory activity and mechanism on lipid production in 3T3-L1 cells by hot water extract derived from Acacia confusa flowers.

IF 2.5 4区 医学 Q3 CHEMISTRY, MEDICINAL Journal of Natural Medicines Pub Date : 2024-10-29 DOI:10.1007/s11418-024-01856-5
Nai-Wen Tsao, Ju-Ya Cheng, Sheng-Yang Wang
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Abstract

Acacia confusa Merr. (Fabaceae) (A. confusa) is a native tree species of Taiwan, commonly found in the low-altitude mountains and hilly areas of the Hengchun Peninsula. This evergreen, perennial, and large-sized tree was the focus of a study that employed various chromatographic and spectroscopic methods to analyze the hot water extract of its flowers. The analysis revealed that the major components of the extract were myricitrin, quercitrin, europetin-3-O-rhamnoside, and chalconaringenin-2'-xyloside, with respective concentrations of approximately 0.22, 0.02, 0.26, and 0.10 mg/g of the flowers. Subsequent cell assays were conducted to assess the inhibitory effect of the extract on lipid synthesis in fat cells. Oil Red O staining results indicated that the extract significantly suppressed fatty acid accumulation in 3T3-L1 cells, with the most pronounced effect observed at a concentration of 180 μg/ml. Furthermore, the hot water extract of A. confusa flowers was found to increase the phosphorylation of AMP-activated protein kinase (AMPK), decrease the phosphorylation of cAMP response element-binding protein (CREB), and reduce the expression of glucocorticoid receptor (GR) protein. This, in turn, inhibited the expression of downstream transcription factors such as CCAT/ehancer binding proteins α (C/EBPα), CCAT/ehancer binding proteins β (C/EBPβ), CCAT/ehancer binding proteins δ (C/EBPδ), peroxisome proliferation-actived receptor γ (PPARγ), and sterol regulatory element binding proteins-1 (SREBP-1). Consequently, the expression of lipid synthesis-related proteins acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and fatty acid translocase (CD36) was reduced, ultimately inhibiting lipid generation. Therefore, the hot water extract of A. confusa flowers shows potential for development as a weight-loss tea.

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探索金合欢花热水提取物对 3T3-L1 细胞脂质生成的抑制活性和机制
金合欢(Acacia confusa Merr.)(豆科)(A. confusa)是台湾的原生树种,常见于恒春半岛的低海拔山区和丘陵地带。这项研究以这种多年生常绿大乔木为重点,采用多种色谱和光谱方法分析其花的热水提取物。分析结果表明,提取物的主要成分是三尖杉酯素、槲皮苷、欧龙血素-3-O-鼠李糖苷和查耳金宁-2'-木糖苷,浓度分别约为 0.22、0.02、0.26 和 0.10 mg/g。随后进行了细胞试验,以评估提取物对脂肪细胞脂质合成的抑制作用。油红 O 染色结果表明,萃取物明显抑制了 3T3-L1 细胞中脂肪酸的积累,在浓度为 180 μg/ml 时效果最明显。此外,研究还发现 A. confusa 花的热水提取物能增加 AMP 激活蛋白激酶(AMPK)的磷酸化,降低 cAMP 反应元件结合蛋白(CREB)的磷酸化,减少糖皮质激素受体(GR)蛋白的表达。这反过来又抑制了下游转录因子的表达,如 CCAT/癌细胞结合蛋白α(C/EBPα)、CCAT/癌细胞结合蛋白β(C/EBPβ)、CCAT/癌细胞结合蛋白δ(C/EBPδ)、过氧化物酶体增殖受体γ(PPARγ)和固醇调节元件结合蛋白-1(SREBP-1)。因此,脂质合成相关蛋白乙酰-CoA 羧化酶(ACC)、脂肪酸合成酶(FAS)和脂肪酸转运酶(CD36)的表达减少,最终抑制了脂质的生成。因此,A. confusa 花的热水提取物具有开发为减肥茶的潜力。
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来源期刊
CiteScore
6.90
自引率
3.00%
发文量
79
审稿时长
1.7 months
期刊介绍: The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.
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