Variability of 7K and 11K SomaScan Plasma Proteomics Assays.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-10-30 DOI:10.1021/acs.jproteome.4c00667

Julián Candia, Giovanna Fantoni, Francheska Delgado-Peraza, Nader Shehadeh, Toshiko Tanaka, Ruin Moaddel, Keenan A Walker, Luigi Ferrucci

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Abstract

SomaScan is an aptamer-based proteomics assay designed for the simultaneous measurement of thousands of human proteins with a broad range of endogenous concentrations. The 7K SomaScan assay has recently been expanded into the new 11K version. Following up on our previous assessment of the 7K assay, here, we expand our work on technical replicates from donors enrolled in the Baltimore Longitudinal Study of Aging. By generating SomaScan data from a second batch of technical replicates in the 7K version as well as additional intra- and interplate replicate measurements in the new 11K version using the same donor samples, this work provides useful precision benchmarks for the SomaScan user community. Beyond updating our previous technical assessment of the 7K assay with increased statistics, here, we estimate interbatch variability, assess inter- and intraplate variability in the new 11K assay, compare the observed variability between the 7K and 11K assays (leveraging the use of overlapping pairs of technical replicates), and explore the potential effects of sample storage time (ranging from 2 to 30 years) in the assays' precision.

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7K 和 11K SomaScan 血浆蛋白质组学检测的变异性。

SomaScan 是一种基于适配体的蛋白质组学检测方法，设计用于同时检测数千种具有广泛内源性浓度的人类蛋白质。7K SomaScan 分析法最近扩展为新的 11K 版本。继之前对 7K 分析法的评估之后，我们在这里进一步扩展了对巴尔的摩老龄化纵向研究（Baltimore Longitudinal Study of Aging）中供体的技术重复数据的研究。通过在 7K 版本中生成第二批技术复制的 SomaScan 数据，以及在新的 11K 版本中使用相同的供体样本进行额外的板内和板间复制测量，这项工作为 SomaScan 用户社区提供了有用的精度基准。除了用更多的统计数据更新我们之前对 7K 检测法的技术评估之外，我们还在这里估算了批次间的变异性，评估了新的 11K 检测法中板间和板内的变异性，比较了 7K 和 11K 检测法之间观察到的变异性（利用重叠的成对技术重复样本），并探讨了样本储存时间（从 2 年到 30 年不等）对检测法精度的潜在影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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