Cerium oxide nanoparticles-assisted aptasensor for chronic myeloid leukaemia detection.

IF 3.4 Q2 CHEMISTRY, MEDICINAL ADMET and DMPK Pub Date : 2024-08-18 eCollection Date: 2024-01-01 DOI:10.5599/admet.2404
Yuspian Nur, Muhammad Ihda Hl Zein, Irkham Irkham, Shabarni Gaffar, Toto Subroto, Yeni Wahyuni Hartati
{"title":"Cerium oxide nanoparticles-assisted aptasensor for chronic myeloid leukaemia detection.","authors":"Yuspian Nur, Muhammad Ihda Hl Zein, Irkham Irkham, Shabarni Gaffar, Toto Subroto, Yeni Wahyuni Hartati","doi":"10.5599/admet.2404","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and purpose: </strong>Chronic myeloid leukaemia (CML) is one of the most lethal types of leukaemia and can rapidly progress if not treated properly. Therefore, having an effective diagnostic strategy is crucial. Various methods are available for diagnosis, including electrochemical biosensors with aptamer bioreceptors.</p><p><strong>Experimental approach: </strong>In this study, we immobilized the KK1D04 aptamer on a screen-printed carbon electrode (SPCE) supported by CeO<sub>2</sub> nanoparticles (CeO<sub>2</sub>NPs) to detect K562 cells, a type of CML cell line. Several parameters were optimized to enhance the aptasensor response using the Box-Behnken experimental design.</p><p><strong>Key results: </strong>The developed aptasensor demonstrated good performance with a limit of detection (LOD) and limit of quantification (LOQ) of 16 cells/mL and 3,882 cells/mL, respectively, in the K562 cell concentration range of 10<sup>2</sup> to 10<sup>6</sup> cells/mL. The optimum experimental conditions were an aptamer concentration of 0.8 ppm, an aptamer incubation time of 36 minutes, and a K562 aptamer-cell incubation time of 13 minutes. The aptasensor also exhibits selectivity for K562 cells compared to Vero cells, THP1 cells, and Raji cells.</p><p><strong>Conclusion: </strong>The aptasensor in this study demonstrated the potential to detect K562 cells. These results could contribute to the advancement of point-of-care (POC) devices for the detection of CML.</p>","PeriodicalId":7259,"journal":{"name":"ADMET and DMPK","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11517519/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ADMET and DMPK","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5599/admet.2404","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0

Abstract

Background and purpose: Chronic myeloid leukaemia (CML) is one of the most lethal types of leukaemia and can rapidly progress if not treated properly. Therefore, having an effective diagnostic strategy is crucial. Various methods are available for diagnosis, including electrochemical biosensors with aptamer bioreceptors.

Experimental approach: In this study, we immobilized the KK1D04 aptamer on a screen-printed carbon electrode (SPCE) supported by CeO2 nanoparticles (CeO2NPs) to detect K562 cells, a type of CML cell line. Several parameters were optimized to enhance the aptasensor response using the Box-Behnken experimental design.

Key results: The developed aptasensor demonstrated good performance with a limit of detection (LOD) and limit of quantification (LOQ) of 16 cells/mL and 3,882 cells/mL, respectively, in the K562 cell concentration range of 102 to 106 cells/mL. The optimum experimental conditions were an aptamer concentration of 0.8 ppm, an aptamer incubation time of 36 minutes, and a K562 aptamer-cell incubation time of 13 minutes. The aptasensor also exhibits selectivity for K562 cells compared to Vero cells, THP1 cells, and Raji cells.

Conclusion: The aptasensor in this study demonstrated the potential to detect K562 cells. These results could contribute to the advancement of point-of-care (POC) devices for the detection of CML.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
用于检测慢性骨髓性白血病的纳米氧化铈辅助传感器。
背景和目的:慢性髓性白血病(CML)是最致命的白血病类型之一,如果治疗不当,病情会迅速恶化。因此,有效的诊断策略至关重要。目前有多种诊断方法,包括带有适配体生物受体的电化学生物传感器:在这项研究中,我们将 KK1D04 合体固定在由 CeO2 纳米粒子(CeO2NPs)支持的丝网印刷碳电极(SPCE)上,以检测 K562 细胞(一种 CML 细胞系)。采用 Box-Behnken 实验设计优化了多个参数,以提高灵敏度:主要结果:所开发的适配传感器性能良好,在K562细胞浓度为102至106个/毫升的范围内,其检测限(LOD)和定量限(LOQ)分别为16个细胞/毫升和3882个细胞/毫升。最佳实验条件为:适配体浓度为 0.8 ppm,适配体孵育时间为 36 分钟,K562 适配体-细胞孵育时间为 13 分钟。与 Vero 细胞、THP1 细胞和 Raji 细胞相比,该适配体传感器对 K562 细胞也具有选择性:结论:本研究中的适配体传感器具有检测 K562 细胞的潜力。结论:本研究中的适配传感器证明了其检测 K562 细胞的潜力,这些结果将有助于提高用于检测 CML 的护理点 (POC) 设备的水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
ADMET and DMPK
ADMET and DMPK Multiple-
CiteScore
4.40
自引率
0.00%
发文量
22
审稿时长
4 weeks
期刊介绍: ADMET and DMPK is an open access journal devoted to the rapid dissemination of new and original scientific results in all areas of absorption, distribution, metabolism, excretion, toxicology and pharmacokinetics of drugs. ADMET and DMPK publishes the following types of contributions: - Original research papers - Feature articles - Review articles - Short communications and Notes - Letters to Editors - Book reviews The scope of the Journal involves, but is not limited to, the following areas: - physico-chemical properties of drugs and methods of their determination - drug permeabilities - drug absorption - drug-drug, drug-protein, drug-membrane and drug-DNA interactions - chemical stability and degradations of drugs - instrumental methods in ADMET - drug metablic processes - routes of administration and excretion of drug - pharmacokinetic/pharmacodynamic study - quantitative structure activity/property relationship - ADME/PK modelling - Toxicology screening - Transporter identification and study
期刊最新文献
Determination of methotrexate using carbon paste electrode modified with ionic liquid/Ni-Co layered double hydroxide nanosheets as a voltammetric sensor. Food and bile micelle binding of zwitterionic antihistamine drugs. Cerium oxide nanoparticles-assisted aptasensor for chronic myeloid leukaemia detection. The biological applications of IPN hydrogels. Investigation of magnesium aluminometasilicate (Neusilin US2) based surface solid dispersion of sorafenib tosylate using QbD approach: In vitro and in vivo pharmacokinetic study
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1