A systematic study of sheep faeces to be used as an alternative inoculum source in the Hohenheim gas test.

IF 2 3区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Archives of Animal Nutrition Pub Date : 2024-06-01 Epub Date: 2024-10-30 DOI:10.1080/1745039X.2024.2417444
Lena Rippstein, Natascha Titze, Katharina Judith Wild, Markus Rodehutscord
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Abstract

In vitro studies of gas production (GP) using rumen fluid obtained from rumen-cannulated animals are common in feed evaluation for ruminants. The objective was to compare sheep faeces with rumen fluid from dairy cows as inocula in the Hohenheim gas test (HGT) using a large number of different-quality feeds. It then was evaluated whether GP obtained by using rumen inoculum (RI-GP) can be estimated from GP using faecal inoculum (FI-GP). Ninety feeds for ruminants including roughages (n = 27), total mixed rations (n = 6), commercial compound feeds for dairy cows (n = 18), cereal grains (n = 10), energy-rich by-products (n = 5), legume grains (n = 6), oilseed meals (n = 6), and other protein-rich feeds (n = 12) were incubated with either rumen inoculum (RI) or faecal inoculum (FI), and GP was recorded at 2, 4, 8, 12, 24, 48, and 72 h. Nonlinear equations were fitted to the GP data to determine GP kinetic parameters. Correlations between RI-GP and FI-GP were calculated for different time points. Linear regressions were calculated to estimate RI-GP from FI-GP using the entire data set or subsets of roughages and concentrates. GP kinetic parameters were lower for all feed categories when incubated with FI instead of RI. On average of all feed samples, the potential GP was 9 mL/200 mg dry matter (DM) lower and the rate of GP was 3.1%/h lower with FI than RI. The estimation of kinetic data for FI included a lag phase of 1.51 h on average, whereas no lag phase was estimated for RI. Estimated parameters indicated an overall lower fermentation activity of FI than RI. However, there was a very similar progression of GP curves of RI-GP and FI-GP. RI-GP after 24 h was significantly correlated with FI-GP in the time period of 24 to 60 h (r = 0.973 - 0.982, including all feed samples). Linear regression analysis showed that RI-GP after 24 h can be estimated from FI-GP after 24 h (Slope = 1.02, R2 = 0.97) and 48 h (Slope = 1.1, R2 = 0.97) including the complete data set. Splitting the data set into roughages and concentrate feeds did not lead to distinctly higher estimation accuracy. In conclusion, sheep faeces can be used as an alternative inoculum for studying a wide range of different-quality feedstuffs. The standard gas test may be modified without the need for rumen-cannulated animals.

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对羊粪便作为霍恩海姆气体试验的替代接种源进行系统研究。
在反刍动物饲料评估中,使用从瘤胃中获得的瘤胃液进行体外产气(GP)研究很常见。这项研究的目的是在霍恩海姆产气试验(HGT)中使用大量不同质量的饲料,比较绵羊粪便和奶牛瘤胃液作为接种体的效果。然后评估使用瘤胃接种体(RI-GP)获得的 GP 是否可以通过使用粪便接种体(FI-GP)获得的 GP 进行估算。90 种反刍动物饲料,包括粗饲料(n = 27)、全混日粮(n = 6)、奶牛商业配合饲料(n = 18)、谷物(n = 10)、富含能量的副产品(n = 5)、豆类谷物(n = 6)、油籽粕(n = 6)和其他富含蛋白质的饲料(n = 12)与瘤胃接种体(RI)或粪便接种体(FI)一起培养,并在 2、4、8、12、24、48 和 72 小时记录 GP。对 GP 数据进行非线性方程拟合,以确定 GP 动力参数。计算了不同时间点的 RI-GP 和 FI-GP 之间的相关性。使用整个数据集或粗饲料和精饲料子集计算线性回归,根据 FI-GP 估算 RI-GP。用 FI 而不是 RI 培养时,所有饲料类别的 GP 动力参数都较低。平均而言,所有饲料样品的潜在 GP 均比 RI 低 9 mL/200 mg 干物质(DM),FI 的 GP 速率比 RI 低 3.1%/h。对 FI 动力学数据的估计包括平均 1.51 小时的滞后期,而对 RI 没有滞后期的估计。估计参数表明,FI 的总体发酵活性低于 RI。然而,RI-GP 和 FI-GP 的 GP 曲线发展非常相似。24 小时后的 RI-GP 与 24 至 60 小时内的 FI-GP 显著相关(r = 0.973 - 0.982,包括所有饲料样品)。线性回归分析表明,24 小时后的 RI-GP(斜率 = 1.02,R2 = 0.97)和 48 小时后的 FI-GP(斜率 = 1.1,R2 = 0.97)(包括完整的数据集)可以估算。将数据集分为粗饲料和精饲料并不能明显提高估算精度。总之,羊粪便可用作研究各种不同质量饲料的替代接种体。标准气体测试可进行修改,而无需对动物进行瘤胃封存。
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来源期刊
Archives of Animal Nutrition
Archives of Animal Nutrition 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
31
审稿时长
>24 weeks
期刊介绍: Archives of Animal Nutrition is an international journal covering the biochemical and physiological basis of animal nutrition. Emphasis is laid on original papers on protein and amino acid metabolism, energy transformation, mineral metabolism, vitamin metabolism, nutritional effects on intestinal and body functions in combination with performance criteria, respectively. It furthermore deals with recent developments in practical animal feeding, feedstuff theory, mode of action of feed additives, feedstuff preservation and feedstuff processing. The spectrum covers all relevant animal species including food producing and companion animals, but not aquatic species. Seldom can priority be given to papers covering more descriptive studies, even if they may be interesting and technically sound or of impact for animal production, or for topics of relevance for only particular regional conditions.
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