Comparative Analysis of Phytochemicals and Antioxidant Characterization Among Different Parts of Catharanthus roseus: In Vitro and In Silico Investigation.

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS Biochemistry Research International Pub Date : 2024-10-18 eCollection Date: 2024-01-01 DOI:10.1155/2024/1904029
Farjana Akter Hira, Ashekul Islam, Kanika Mitra, Ummey Hafsa Bithi, Khondoker Shahin Ahmed, Sanzida Islam, Shaike Mohammad Abdullah, Md Nazim Uddin
{"title":"Comparative Analysis of Phytochemicals and Antioxidant Characterization Among Different Parts of <i>Catharanthus roseus</i>: In Vitro and In Silico Investigation.","authors":"Farjana Akter Hira, Ashekul Islam, Kanika Mitra, Ummey Hafsa Bithi, Khondoker Shahin Ahmed, Sanzida Islam, Shaike Mohammad Abdullah, Md Nazim Uddin","doi":"10.1155/2024/1904029","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background:</b> The study investigates the antioxidant properties of <i>Catharanthus roseus</i>, focusing on identifying its antioxidant compounds and chemical constituents. We compare antioxidant activities across its root, stem, flower, and leaf and examine the inhibition of reactive oxygen species (ROS)-generating enzymes by the plant's phytocompounds. <b>Methods:</b> We conducted a comprehensive analysis that included proximate analysis, mineral content assessment, and in vitro antioxidant characterization of various plant parts-root, stem, flower, and leaf. The levels of bioactive phytochemicals in both ethanol and mixed-solvent extracts of <i>Catharanthus roseus</i> were quantified using high-performance liquid chromatography with a diode array detector (HPLC-DAD). Additionally, we performed molecular docking studies to explore the interactions of quantified phytocompounds. <b>Results:</b> HPLC-DAD analysis quantified catechin hydrate, catechol, (-) epicatechin, rutin hydrate, trans-cinnamic acid, quercetin, vanillic acid, kaempferol, and trans-ferulic acid in <i>Catharanthus roseus.</i> Despite the ethanol extract having higher total antioxidant properties and flavonoid content, the mixed-solvent extract exhibited higher EC<sub>50</sub> for reducing power and lower IC<sub>50</sub> for ABTS, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and metal chelating activities. Molecular docking studies indicated that compounds such as catechin, rutin, epicatechin, quercetin, and kaempferol significantly inhibit the ROS-generating enzyme microsomal prostaglandin E synthase 1 (mPGES-1). <b>Conclusions:</b> The mixed-solvent extract had higher levels of catechin hydrate, rutin hydrate, trans-ferulic acid, and vanillic acid, whereas the ethanol extract contained more (-) epicatechin, catechol, kaempferol, quercetin, and trans-cinnamic acid. While the extracts displayed antioxidant activity, the phytoconstituents also inhibited ROS-generating mPGES-1. These results identify key compounds with potential for developing new chemotherapeutic agents against ROS.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519068/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry Research International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/1904029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Background: The study investigates the antioxidant properties of Catharanthus roseus, focusing on identifying its antioxidant compounds and chemical constituents. We compare antioxidant activities across its root, stem, flower, and leaf and examine the inhibition of reactive oxygen species (ROS)-generating enzymes by the plant's phytocompounds. Methods: We conducted a comprehensive analysis that included proximate analysis, mineral content assessment, and in vitro antioxidant characterization of various plant parts-root, stem, flower, and leaf. The levels of bioactive phytochemicals in both ethanol and mixed-solvent extracts of Catharanthus roseus were quantified using high-performance liquid chromatography with a diode array detector (HPLC-DAD). Additionally, we performed molecular docking studies to explore the interactions of quantified phytocompounds. Results: HPLC-DAD analysis quantified catechin hydrate, catechol, (-) epicatechin, rutin hydrate, trans-cinnamic acid, quercetin, vanillic acid, kaempferol, and trans-ferulic acid in Catharanthus roseus. Despite the ethanol extract having higher total antioxidant properties and flavonoid content, the mixed-solvent extract exhibited higher EC50 for reducing power and lower IC50 for ABTS, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and metal chelating activities. Molecular docking studies indicated that compounds such as catechin, rutin, epicatechin, quercetin, and kaempferol significantly inhibit the ROS-generating enzyme microsomal prostaglandin E synthase 1 (mPGES-1). Conclusions: The mixed-solvent extract had higher levels of catechin hydrate, rutin hydrate, trans-ferulic acid, and vanillic acid, whereas the ethanol extract contained more (-) epicatechin, catechol, kaempferol, quercetin, and trans-cinnamic acid. While the extracts displayed antioxidant activity, the phytoconstituents also inhibited ROS-generating mPGES-1. These results identify key compounds with potential for developing new chemotherapeutic agents against ROS.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
蔷薇科植物不同部位的植物化学成分和抗氧化特性比较分析:体外和体内研究
背景:本研究调查了长春花的抗氧化特性,重点是确定其抗氧化化合物和化学成分。我们比较了其根、茎、花和叶的抗氧化活性,并研究了该植物的植物化合物对活性氧(ROS)生成酶的抑制作用。研究方法我们对植物的各个部分(根、茎、花和叶)进行了全面分析,包括近似分析、矿物质含量评估和体外抗氧化特性鉴定。我们使用带二极管阵列检测器的高效液相色谱法(HPLC-DAD)对石竹的乙醇提取物和混合溶剂提取物中生物活性植物化学物质的含量进行了定量分析。此外,我们还进行了分子对接研究,以探索定量植物化合物之间的相互作用。结果HPLC-DAD 分析定量了儿茶素水合物、儿茶酚、(-) 表儿茶素、芦丁水合物、反式肉桂酸、槲皮素、香草酸、山柰酚和反式阿魏酸。尽管乙醇提取物的总抗氧化性和类黄酮含量较高,但混合溶剂提取物的还原力 EC50 值较高,ABTS、2,2-二苯基-1-苦基肼(DPPH)和金属螯合活性的 IC50 值较低。分子对接研究表明,儿茶素、芦丁、表儿茶素、槲皮素和山柰醇等化合物能显著抑制 ROS 生成酶微粒体前列腺素 E 合酶 1(mPGES-1)。结论混合溶剂提取物中的儿茶素水合物、芦丁水合物、反式阿魏酸和香草酸含量较高,而乙醇提取物中的(-)表儿茶素、儿茶酚、山奈儿茶酚、槲皮素和反式肉桂酸含量较高。在提取物显示出抗氧化活性的同时,植物成分还抑制了产生 ROS 的 mPGES-1。这些结果确定了一些关键化合物,它们具有开发新的抗 ROS 化疗药物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
期刊最新文献
Hypoglycemic Assessment of Aqueous Leaf Extract of Moringa oleifera on Diabetic Wistar Rats. Comparative Analysis of Phytochemicals and Antioxidant Characterization Among Different Parts of Catharanthus roseus: In Vitro and In Silico Investigation. Green Synthesis and Anti-Inflammatory Activity of Silver Nanoparticles Based on Leaves Extract of Aphania senegalensis. Proteomics and Bioinformatics Investigations Link Overexpression of FGF8 and Associated Hub Genes to the Progression of Ovarian Cancer and Poor Prognosis. Effects of Low Concentration of Glyphosate-Based Herbicide on Genotoxic, Oxidative, Inflammatory, and Behavioral Meters in Danio rerio (Teleostei and Cyprinidae).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1