Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Biotechnology Pub Date : 2024-10-30 DOI:10.1186/s12896-024-00909-7
Mai Ali Mwaheb, Yasmeen A Hasanien, Amira G Zaki, Alaa S Abdel-Razek, Laila R Abd Al Halim
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Abstract

Background: Natural pigments are becoming more significant because of the rising cost of raw materials, pollution, and the complexity of synthetic pigments. Compared to synthetic pigments, natural pigments exhibit antimicrobial properties and is less allergic. Pigments from microbial sources could easily be obtained in an inexpensive culture media, produced in high yields, and microbes are capable of producing different colored pigments. Searching for new sources for natural pigments to replace synthetic ones in food applications has become an urgent necessity, but the instability of these compounds is sometimes considered one of the obstacles that reduce their application. Encapsulation provides an ideal solution for natural dye protection through a controlled release strategy. Thus, this study aims at isolation of several soil fungi and subsequent screening their pigment production ability. The chosen pigment-producing fungal strain underwent full identification. The produced pigment was extracted with ethyl acetate and estimated spectrophotometrically. As there is a necessity to obtain a high pigment yield for efficient industrial application, the best production medium was tested, optimum conditions for maximum dye production were also investigated through the response surface methodology, and gamma irradiation was also employed to enhance the fungal productivity. Encapsulation of the produced pigment into chitosan microsphere was tested. The pigment release under different pH conditions was also investigated.

Results: A new strain, Fusarium verticillioides AUMC 15934 was chosen and identified for a violet pigment production process. Out of four different media studied, the tested strain grew well on potato dextrose broth medium. Optimum conditions are initial medium pH 8, 25 °C-incubation temperature, and for 15-day incubation period under shaking state. Moreover, a 400 Gy irradiation dose enhanced the pigment production. Chitosan microsphere loaded by the pigment was successfully prepared and characterized by infrared spectroscopy and scanning electron microscopy.

Conclusion: This irradiated Fusarium strain provides a more economically favorable source for production of a natural violet dye with an optimum productivity, enhanced yield, and improved properties (such as, enhanced stability, controlled release, and bioaccessibility) by encapsulation with chitosan for efficient application in food industry.

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轮状镰刀菌色素:生产、响应面优化、伽马射线照射和封装研究。
背景:由于原材料成本上升、污染和合成颜料的复杂性,天然颜料正变得越来越重要。与合成颜料相比,天然颜料具有抗菌特性,过敏性较低。微生物来源的颜料可以很容易地在廉价的培养基中获得,产量高,而且微生物能够生产不同颜色的颜料。寻找天然色素的新来源以取代食品中的合成色素已成为当务之急,但这些化合物的不稳定性有时被认为是减少其应用的障碍之一。封装技术通过控制释放策略为天然染料的保护提供了理想的解决方案。因此,本研究旨在分离几种土壤真菌,并随后筛选它们的色素生产能力。所选的色素生产真菌菌株经过了全面鉴定。产生的色素用乙酸乙酯提取,并用分光光度法进行估算。由于需要获得较高的色素产量以实现有效的工业应用,因此对最佳生产介质进行了测试,还通过响应面方法研究了染料产量最大化的最佳条件,并采用伽马射线照射来提高真菌的生产率。还测试了将生产的颜料封装到壳聚糖微球中的情况。此外,还研究了不同 pH 值条件下的色素释放情况:结果:选择并鉴定了一株新菌株 Fusarium verticillioides AUMC 15934,用于紫色颜料的生产过程。在所研究的四种不同培养基中,被测菌株在马铃薯葡萄糖肉汤培养基上生长良好。最佳条件是初始培养基 pH 值为 8,培养温度为 25 °C,在振荡状态下培养 15 天。此外,400 Gy 的辐照剂量可提高色素产量。成功制备了负载色素的壳聚糖微球,并通过红外光谱和扫描电子显微镜对其进行了表征:该辐照镰刀菌菌株为生产天然紫色染料提供了一种更经济的来源,通过壳聚糖封装,该染料具有最佳的生产率、更高的产量和更好的性能(如更高的稳定性、可控释放性和生物可及性),可有效地应用于食品工业。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Biotechnology
BMC Biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.60
自引率
0.00%
发文量
34
审稿时长
2 months
期刊介绍: BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.
期刊最新文献
Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress. Limonene encapsulated alginate/collagen as antibiofilm drug against Acinetobacter baumannii. Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies. Kinetic and thermodynamic analysis of alizarin Red S biosorption by Alhagi maurorum: a sustainable approach for water treatment. Biological activities of Hypericum spectabile extract optimized using artificial neural network combined with genetic algorithm application.
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