Menstrual blood-derived mesenchymal stem cells combining with platelet-rich plasma infusion in endometrium repair.

IF 1.6 4区 医学 Q3 OBSTETRICS & GYNECOLOGY Journal of Obstetrics and Gynaecology Research Pub Date : 2024-10-31 DOI:10.1111/jog.16135
Yang Wang, Wenxin Liu, Hui Xu, Liyuan Dong, Yalou Jiao, Zhe Qiao
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Abstract

Objective: Thin endometrium caused by various factors affects the conception rate of females worldwide; however, current medications are still insufficient. Therefore, a novel approach is needed. We previously reported the effect of menstrual blood-derived mesenchymal stem cells (MenSCs) in ameliorating ethanol-induced endometrial injuries. In the present study, we aimed to investigate whether the effect of MenSCs is enhanced by a combination of platelet-rich plasma (PRP) infusion.

Methods: An endometrial injury mouse model was established by infiltrating 95% ethanol for 15 s into the uterus, followed by MenSCs, PRP, or MenSCs + PRP treatment. Pathological changes were observed by HE staining. The expression of CK18, vimentin, ItgαVβ3, and VEGF was determined using IHC staining and WB blotting.

Results: Compared with the model, MenSCs, PRP, and MenSCs + PRP treatments significantly improved endometrial damage and thickness, with the combined therapy displaying the most pronounced efficacy. The density of CK18-, vimentin-, and ItgαVβ3 positive cells increased most significantly in the MenSCs + PRP group of mice. In addition, the protein expression of CK18, vimentin, and VEGF was significantly upregulated after MenSCs + PRP, MenSCs, and PRP treatment, with MenSCs + PRP therapy showing the best efficacy.

Conclusion: MenSCs + PRP therapy is more beneficial for ameliorating ethanol-induced endometrial damage than MenSCs or PRP alone, providing a basis for the investigation of novel approaches for treating thin endometria.

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月经血间充质干细胞结合富血小板血浆注入修复子宫内膜。
目的各种因素导致的子宫内膜薄影响着全球女性的受孕率,但目前的药物治疗仍显不足。因此,需要一种新的方法。我们曾报道过经血间充质干细胞(MenSCs)在改善乙醇引起的子宫内膜损伤方面的作用。在本研究中,我们旨在探讨是否结合富血小板血浆(PRP)输注可增强间充质干细胞的作用:方法:将 95% 的乙醇浸润子宫 15 秒,然后用 MenSCs、PRP 或 MenSCs + PRP 治疗,建立子宫内膜损伤小鼠模型。通过 HE 染色观察病理变化。通过 IHC 染色和 WB 印迹检测 CK18、波形蛋白、ItgαVβ3 和 VEGF 的表达:结果:与模型相比,MenSCs、PRP 和 MenSCs + PRP 治疗能显著改善子宫内膜损伤和厚度,其中联合治疗的疗效最明显。MenSCs+PRP组小鼠CK18、波形蛋白和ItgαVβ3阳性细胞的密度增加最为明显。此外,MenSCs + PRP、MenSCs 和 PRP 治疗后,CK18、波形蛋白和血管内皮生长因子的蛋白表达明显上调,其中 MenSCs + PRP 治疗的疗效最好:结论:MenSCs + PRP疗法比单独使用MenSCs或PRP疗法更有利于改善乙醇引起的子宫内膜损伤,为研究治疗薄型子宫内膜的新方法提供了依据。
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来源期刊
CiteScore
3.10
自引率
0.00%
发文量
376
审稿时长
3-6 weeks
期刊介绍: The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.
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