Xingyu Zeng, Wenyan Zhao, Aihua Deng, Lixuan Xiang, Xuan Tang, Huan Li, Hanbin Yin, Rongjie Huang, Yulong Xiao, Yi Liu, Zui Yao, Yongle Liu, Zhitian Du, Kerui Huang
{"title":"Characterization and phylogenetic analysis of the chloroplast genome of <i>Diospyros</i> aff. <i>oleifera</i>.","authors":"Xingyu Zeng, Wenyan Zhao, Aihua Deng, Lixuan Xiang, Xuan Tang, Huan Li, Hanbin Yin, Rongjie Huang, Yulong Xiao, Yi Liu, Zui Yao, Yongle Liu, Zhitian Du, Kerui Huang","doi":"10.1080/23802359.2024.2419451","DOIUrl":null,"url":null,"abstract":"<p><p>The <i>Diospyros</i> genus (Ebenaceae) has significant economic value. During field surveys, we discovered a <i>Diospyros</i> specimen showing morphological overlap with both <i>D</i>. <i>oleifera</i> and <i>D. kaki</i> var. silvestris, provisionally named <i>Diospyros</i> aff. <i>oleifera</i>. To resolve its taxonomy, we sequenced and analyzed its chloroplast genome. The complete chloroplast genome is 157,732 bp with a quadripartite structure. mVISTA analysis revealed unique sequence variations compared to related species. Phylogenetic analysis using 75 protein-coding genes grouped it with <i>D. oleifera</i>, indicating their close relationship. Our findings suggest this specimen likely represents a novel, undescribed species. This study provides insights into Diospyros diversity and a foundation for future research.</p>","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520100/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/23802359.2024.2419451","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The Diospyros genus (Ebenaceae) has significant economic value. During field surveys, we discovered a Diospyros specimen showing morphological overlap with both D. oleifera and D. kaki var. silvestris, provisionally named Diospyros aff. oleifera. To resolve its taxonomy, we sequenced and analyzed its chloroplast genome. The complete chloroplast genome is 157,732 bp with a quadripartite structure. mVISTA analysis revealed unique sequence variations compared to related species. Phylogenetic analysis using 75 protein-coding genes grouped it with D. oleifera, indicating their close relationship. Our findings suggest this specimen likely represents a novel, undescribed species. This study provides insights into Diospyros diversity and a foundation for future research.
油橄榄属(Ebenaceae)具有重要的经济价值。在野外调查中,我们发现了一个 Diospyros 标本,它与 D. oleifera 和 D. kaki var. silvestris 在形态上有重叠,因此暂时命名为 Diospyros aff.为了解决其分类问题,我们对其叶绿体基因组进行了测序和分析。完整的叶绿体基因组为 157,732 bp,具有四分体结构。利用 75 个蛋白编码基因进行的系统进化分析将其与 D. oleifera 归为一类,表明两者关系密切。我们的研究结果表明,该标本很可能代表了一个未被描述的新物种。这项研究提供了对 Diospyros 多样性的深入了解,为今后的研究奠定了基础。