Mitochondrial DNA. Part B, Resources最新文献

In this study, we conducted a comprehensive sequencing and characterization of the chloroplast genome of the Asian endemic species Calystegia pubescens 'Anestia'. The complete genome is 152,079 bp long, with a GC content of 37.37%. It consists of a large single-copy region (87,891 bp), a small single-copy region (19,888 bp), and a pair of inverted repeats regions (22,149 bp). A total of 126 genes were identified, including 81 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis revealed a close relationship between C. pubescens 'Anestia' and C. hederacea. These results provide valuable genetic insights for species identification and phylogenetic studies within the genus Calystegia.

The complete mitochondrial genome of Rasbora atridorsalis Kottelat and Chu (Spixiana. 10(3):313-318) is first characterized herein (GenBank accession: OM617726.1). The circular mitogenome is 16,524 base pairs (bp) in length, containing 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and two non-coding regions, with an AT bias of 58.7%. A Bayesian phylogenetic reconstruction, based on the amino acid sequences of 13 mitochondrial protein-coding genes from R. atridorsalis and 41 congeneric species, resolved the evolutionary relationships within the genus, providing molecular evidence for intra-generic affinities. This work fills a critical genomic gap in Rasbora phylogenetics, provides a molecular basis for taxonomic revisions, and enhances understanding of the evolutionary history and interspecific relationships in Rasborinae.

Anemone sylvestris Linnaeus (1753) is a species in the Ranunculaceae family within the genus Anemone. It is mainly distributed in China, Croatia, and Poland. The chloroplast genome has a total length of 161,033 bp, comprising a large single-copy region of 81,144 bp, a small single-copy region of 17,393 bp, and two inverted repeat regions of 31,248 bp each. The GC content is 37.54%. A total of 134 genes were annotated, including 90 protein-coding genes, 8 rRNA genes, and 36 tRNA genes. Phylogenetic analysis using the maximum likelihood method robustly supported the close relationship of A. sylvestris with other Anemone species, namely A. raddeana, A. reflexa, and A. altaica, forming a well-defined monophyletic clade. The complete chloroplast genome of A. sylvestris reported here provides a useful resource for subsequent phylogenetic, identification, and resource related studies.

Globba racemosa, a Zingiberaceae species native to southern and southwestern China, the complete chloroplast genome of G. racemosa was sequenced and reported for the first time. The genome was 163,321 bp in total length, possess typical quadripartite structures that consist of a large single copy (LSC, 88,111 bp), a small single copy (SSC, 15,770 bp) and a pair of inverted repeats (IRs, 29,720 bp). The complete chloroplast genome contained 134 genes (87 protein-coding, 39 tRNA, 8 rRNA), with an overall GC content of 35.8%. phylogenetic analysis based on 10 chloroplast genomes indicated its close affinity to R. multiflora in Glolla. This newly reported chloroplast genome can provide fundamental genetic data for future research on the genus Globba.

Fraxinus paxiana distributed in Shaanxi, Gansu, Hubei, Hunan, and Sichuan provinces, is used as a local medicine in Shaanxi and also serves as an adulterant of traditional Chinese medicine Qinpi. Here, we assembled its 155,692 bp chloroplast genome (GC content 37.87%) with a typical quadripartite structure. A total of 133 genes were annotated, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes. We identified 43 SSRs, predominantly composed of mononucleotide repeats. Phylogenetic analysis showed that the genus Fraxinus is divided into two branches, with F. paxiana and F. insularis having the closest phylogenetic relationship.

Seseli giganteum Lipsky, an endemic Apiaceae species from the Kyrgyz Republic, was characterized based on its complete plastid genome. The circular genome is 147,554 bp long and exhibits a typical quadripartite structure, comprising an LSC (92,927 bp), SSC (17,631 bp), and two IRs (18,498 bp each). Phylogenetic analyses using 79 plastid protein-coding genes under maximum likelihood and Bayesian inference yielded highly congruent trees with strong support. The results indicate that Seseli L. is non-monophyletic, with Ligusticum L. and Peucedanum L. nested within it. S. giganteum is sister to S. valentinae Popov, highlighting a close Central Asian relationship and suggesting a need to reassess generic boundaries.

This study reports the first complete mitochondrial genome of Opsariichthys jishuiensis. The circular genome (16,614 bp) contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. Phylogenetic analysis using Bayesian inference and maximum-likelihood methods identifies O. jishuiensis as most closely related to O. acutipinnis and O. evolans, forming a monophyletic clade within the subfamily Xenocyprinae. These findings confirm the taxonomic placement of Opsariichthys and provide essential genetic resources for the evolutionary biology, taxonomy, and conservation of freshwater fishes in the Huanghe-Huaihe River Basin.

Actinidia melliana is a unique Actinidia species whose genomic data is extremely lacking. In this study, we reported the complete chloroplast genome of A. melliana for the first time, which has a total length of 156,159 bp, consisting of a large single-copy (LSC) region of 90,525 bp, a small single-copy (SSC) region of 20,578 bp, and a pair of inverted repeat (IR) regions with 22,528 bp each. A total of 133 genes were annotated, including 84 protein-coding genes, 41 transfer RNA genes (tRNA) and 8 ribosomal RNA genes (rRNA). Phylogenetic analysis alongside 28 other species in the Actinidiaceae family revealed that A. melliana is most closely related to A. cylindrica var. cylindrica. This study provides new genetic resources for evolutionary studies of Actinidia and supports future efforts in species identification and conservation within the genus.

The clearwing moth Synanthedon namdoelegans Kim, Kim and Choi, 2025 (Lepidoptera: Sesiidae) was newly discovered in South Korea in 2024. We sequenced 15,578 bp-long complete mitochondrial genome of the species. It contained the trnQ-trnS2-trnM-trnI arrangement at the A + T-rich region and ND2 junction (the underline indicates counterclockwise direction) unique to tribe Synanthedonini. Phylogenetic analyses of 18 Cossoidea species supported the monophyly of each Synanthedonini and Synanthedon, and revealed a sister relationship between S. namdoelegans and Synanthedon bicingulata (Staudinger 1887). These data will be useful in taxonomic, molecular ecological, and pest management research.

The Zygaenidae family comprises diurnal moths recognized for their bright coloration and chemical defenses. Despite its ecological and evolutionary significance, mitochondrial genome (mitogenome) data remain scarce. Here, we present the mitogenome of Neochalcosia remota, the first to be elucidated in members of the Neochalcosia genus, in the Chalcosiinae subfamily. The complete mitogenome is 15,246 bp and contains the standard set of 37 genes, with 13 protein-coding genes (PCGs), 22 tRNAs, and 2 rRNAs, as well as an A + T-rich region. An inverted translocation of the trnE-trnS₁ block (underlined gene names indicate a counter-clockwise transcriptional direction), instead of the typical trnS₁ -trnE arrangement in Lepidoptera, present in all Zygaenidae species, was also detected in N. remota. Phylogenetic analyses based on the 13 PCGs and two rRNAs in the Zygaenidae strongly supported the monophylies of this family and its subfamilies (Chalcosiinae, Procridinae, and Zygaeninae), placing N. remota as a sister taxon to Eterusia aedea. As a new contribution to the mitogenome database, the mitogenome of N. remota could provide valuable genetic data leading to a diverse taxonomic hierarchy in phylogenetic studies.