Mitochondrial DNA. Part B, Resources最新文献

The complete mitogenome of Orthotomus sutorius Pennant, 1769 this species was sequenced using an Illumina Novaseq platform. It is 16,982 bp in length and consists of 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes (12S rRNA and 16S rRNA) and a control region (CR). We reconstructed a phylogenetic tree based on the mitogenome sequences of 10 species from family Cisticolidae with its adjacent families, as well as one outgroup. Phylogenetic analysis indicated that Orthotomus sutorius is well clustered with Cisticola juncidis. The new mitogenome data would provide useful information for evolutionary research on O. sutorius and the family Cisticolidae.

In this study, we assembled and annotated the mitochondrial genome (mitogenome) of B. oleracea L. var. italica Plenck. This mitogenome was found to span 219 964 bp, with a GC content of 45.25%. It comprised 61 genes, including 35 protein-coding, 23 transfer RNA, and three ribosomal RNA genes. Among these, only 11 genes contained introns. Codon preference analysis revealed a bias toward codons ending in A/U bases. A phylogenetic analysis demonstrated a close relationship between B. oleracea L. var. italica Plenck, B. oleracea L. botrytis, and B. oleracea var. capitata. This reference mitogenome provides a basis for research on genetic conservation, phylogenetic relationships, and molecular breeding strategies among members of the Brassica genus.

Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomelidae) is a horticultural pest with a widespread distribution. However, the complete mitochondrial genome (mitogenome) of this species has not been available . In this study, we sequenced and analyzed the first complete mitogenome of T. japana. The mitogenome is 15,681 bp in length (GenBank accession number: OR387477) and includes 13 typicalPCGs, 22 tRNAs, two rRNAs, and two control regions. Furthermore, phylogenetic analysis indicated that T. japana is closely related to Basilepta melanopus, B. fulvipes, and Colasposoma dauricum. The T. japana mitogenome data provides valuable insights into the genetic evolution of this species .

Halichoeres margaritaceus Valenciennes 1839 is a polygynous species of coral fish. We sequenced the complete mitochondrial genome of H. margaritaceus, and conducted a comprehensive analysis to reveal its characteristics. Results showed that the mitochondrial genome is a closed circular molecule comprising 16,710 bp. Phylogenetic analysis based on mitochondrial whole genome sequences revealed H. margaritaceus as a sister group of H. ornatissimus, H. tenuispinis, etc., with maximal support. This study presents, for the first time, the complete mitochondrial genome of H. margaritaceus, offering valuable insights that can aid in future research on the biodiversity and conservation management of this species.

Vicia kulingana L. H. Bailey 1920, a famous edible and medicinal plant, had its chloroplast genome sequenced in this study to determine its phylogenetic relationship with other related species. The research findings are as follows: 1. The chloroplast genome of V. kulingana was 125, 696 bp long and lacked an inverted repeat unit. 2. The genome contains a total of 102 genes, including 69 protein-coding genes, 28 tRNAs, and 5 ribosomal RNA. 3. Phylogenetic analysis revealed that V. kulingana and other three Vicia species are genetically closely related and are located on the same branch of the phylogenetic tree, indicating a sister group relationship among them. Our results support that Lens was treated as a synonym of Vicia. This study provides a foundation for the identification, classification, and exploration of genetic diversity within the Vicia genus.

Polygala tatarinowii (Polygalaceae) is an annual herb with ecological, medicinal, and agricultural potential. Here, we present the inaugural assembly of its chloroplast genome (plastome). The total plastome spans 168,779 bp in length, featuring two expanded inverted repeat (IR) regions measuring 43,427 bp each, flanking a large single-copy (LSC) region of 73,712 bp and a small single-copy (SSC) region of 8213 bp. The plastome exhibits a GC content of 36.8%. Annotation of the plastome revealed a total of 112 unique genes, comprising 78 protein-coding genes, 30 tRNAs and 4 rRNAs. Uncommonly, two copies of the trnQ^UUG gene were identified within the IRs, resulting in the presence of three trnQ^UUG copies in this plastome. Additionally, reductions were observed in the ycf1 and ycf2 genes. Phylogenetic analysis effectively resolves the relationships among sampled Polygalaceae species. Our findings underscore the instrumental role of plastomes in advancing research on the systematics, phylogenetics, and genetic diversity of Polygalaceae.

The genus Petrocodon is uniquely distributed in karst areas and exhibits high floral morphological diversity. We assembled and characterized the complete chloroplast genomes of Petrocodon mirus X.Z.Shi, J.X.Fu & L.H.Yang 2024 and Petrocodon hancei (Hemsl. 1890) A.Weber & Mich.Möller 2011. The genome sizes are 153,547 bp and 153,294 bp, respectively. Phylogenetic analysis revealed that P. hancei is closely related to P. multiflorus, while the position of P. mirus remains unclear. These findings provide genomic resources for studying genetic diversity in Petrocodon and Gesneriaceae.

Paraisaria gracilioides (Kobayasi) Luangsa-ard, Mongkolsamrit & Samson, originally described as Ophiocordyceps gracilioides, is a member of the Ophiocordycipitaceae, within the order Hypocreales. This study, presented the mitochondrial genome of P. gracilioides structured as a circular molecule spanning 181,140 bp, was larger than those of most Ophiocordycipitaceae species. Despite this size variation, encoding 49 genes, including 15 PCGs, 26 tRNAs, two rRNAs, and six ORFs. Phylogenetic analysis based on nucleotide sequences of 14 PCGs revealed that P. gracilioides clustered with Paraisaria gracilis, forming a subgroup within Ophiocordyceps species. The results contribute to refining the taxonomic framework of Ophiocordycipitaceae.

The complete chloroplast genome of S. sinkiangensis is 152,621 bp in length, comprising LSC (84,011 bp), SSC (18,630 bp), and IR (24,990 bp each) regions. The genome has 36.6% GC content (LSC: 34.6%, SSC: 29.4%, IR: 42.7%) and encodes 133 genes (88 protein-coding, 37 tRNA, eight rRNA). Phylogenetic analysis (ML/BI) indicate S. sinkiangensis is closest to S. collina. This study provides genomic insights and clarifies its phylogenetic position within Amaranthaceae.

Glyptotendipes pallens (Meigen, 1804) serves as a significant bioindicator for monitoring freshwater ecosystems. Despite the performance of COI barcode analyses on G. pallens, the mitogenome of this taxon remains unassembled and unanalyzed. In this study, we provide one newly sequenced complete mitogenome of G. pallens for the first time. The mitogenome is 16,063 bp long with high A + T content, and consists of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a noncoding control region. The phylogenetic analysis strongly supports G. pallens and G. tokunagai as sister species.