A phosphate starvation induced small RNA promotes Bacillus biofilm formation.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY npj Biofilms and Microbiomes Pub Date : 2024-10-29 DOI:10.1038/s41522-024-00586-6

Yulong Li, Xianming Cao, Yunrong Chai, Ruofu Chen, Yinjuan Zhao, Rainer Borriss, Xiaolei Ding, Xiaoqin Wu, Jianren Ye, Dejun Hao, Jian He, Guibin Wang, Mingmin Cao, Chunliang Jiang, Zhengmin Han, Ben Fan

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Abstract

Currently, almost all known regulators involved in bacterial phosphorus metabolism are proteins. In this study, we identified a conserved new small regulatory RNA (sRNA), named PhoS, encoded in the 3' untranslated region (UTR) of the phoPR genes in Bacillus velezensis and B. subtilis. Expression of phoS is strongly induced upon phosphorus scarcity and stimulated by the transcription factor PhoP. Conversely, PhoS positively regulates PhoP translation by binding to the ribosome binding site (RBS) of phoP mRNA. PhoS can promote Bacillus biofilm formation through, at least in part, enhancing the expression of the matrix-related genes, such as the eps genes and the tapA-sipW-tasA operon. The positive regulation of phoP expression by PhoS contributes to the promoting effect of PhoS on biofilm formation. sRNAs regulating biofilm formation have rarely been reported in gram-positive Bacillus species. Here we highlight the significance of sRNAs involved in two important biological processes: phosphate metabolism and biofilm formation.

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磷酸盐饥饿诱导的小核糖核酸可促进芽孢杆菌生物膜的形成。

目前，几乎所有参与细菌磷代谢的已知调控因子都是蛋白质。在这项研究中，我们发现了一种保守的新的小调控 RNA（sRNA），名为 PhoS，编码于枯草芽孢杆菌和枯草杆菌 phoPR 基因的 3' 非翻译区（UTR）。缺磷时，转录因子 PhoP 会强烈诱导 phoS 的表达。相反，PhoS 通过与 phoP mRNA 的核糖体结合位点（RBS）结合，积极调节 PhoP 的翻译。PhoS 可以促进芽孢杆菌生物膜的形成，至少部分是通过增强基质相关基因的表达，如 eps 基因和 tapA-sipW-tasA 操作子。PhoS 对 phoP 表达的正向调控有助于 PhoS 对生物膜形成的促进作用。在革兰氏阳性芽孢杆菌中，很少有调控生物膜形成的 sRNA 的报道。在此，我们强调了参与磷酸盐代谢和生物膜形成这两个重要生物过程的 sRNA 的重要性。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.