Validation of a triiodothyronine (T3) ELISA for mouse fecal samples.

IF 2.2 Q3 PHYSIOLOGY Physiological Reports Pub Date : 2024-11-01 DOI:10.14814/phy2.70115
Lucia M Thompson, Brailey M Coulter, Cinnamon L VanPutte
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Abstract

Acquiring sufficient blood for hormone analysis in mice can be a limiting step. Hormone analysis techniques using non-invasive sample collection have been vigorously developed for endangered species, from whom blood sampling is prohibited, or from species that are otherwise difficult to handle in a laboratory setting. Because there are interactions between glucocorticoids and thyroid hormones (T3 and T4), reducing the animal's "distress" during sample collection is imperative. Measurement of fecal T3 provides less sensitive, baseline information regarding thyroid function while permitting a non-invasive technique for more frequent sampling. We demonstrated that using a methanol extraction protocol produced the most reliable fecal T3 measurement in an enzyme-linked immunosorbent assay (ELISA). We found that during a thyroid hormone-treated state, fecal and plasma T3 measurements from mice are directly related, while during a methimazole-treated state, fecal and plasma T3 measurements from mice are inversely related. Fecal samples are a useful way to monitor thyroid hormone function in laboratory mice.

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验证小鼠粪便样本中的三碘甲状腺原氨酸 (T3) 酶联免疫吸附试验。
获取足够的小鼠血液用于激素分析可能是一个限制性步骤。针对禁止采血的濒危物种或难以在实验室环境中处理的物种,人们大力开发了使用非侵入性样本采集的激素分析技术。由于糖皮质激素和甲状腺激素(T3 和 T4)之间存在相互作用,因此必须减少动物在样本采集过程中的 "痛苦"。粪便 T3 测量可提供灵敏度较低的甲状腺功能基线信息,同时允许采用无创技术进行更频繁的采样。我们证明,在酶联免疫吸附试验(ELISA)中,使用甲醇提取方案能获得最可靠的粪便 T3 测量结果。我们发现,在甲状腺激素治疗状态下,小鼠粪便和血浆 T3 测量值直接相关,而在甲巯咪唑治疗状态下,小鼠粪便和血浆 T3 测量值成反比。粪便样本是监测实验鼠甲状腺激素功能的有效方法。
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来源期刊
Physiological Reports
Physiological Reports PHYSIOLOGY-
CiteScore
4.20
自引率
4.00%
发文量
374
审稿时长
9 weeks
期刊介绍: Physiological Reports is an online only, open access journal that will publish peer reviewed research across all areas of basic, translational, and clinical physiology and allied disciplines. Physiological Reports is a collaboration between The Physiological Society and the American Physiological Society, and is therefore in a unique position to serve the international physiology community through quick time to publication while upholding a quality standard of sound research that constitutes a useful contribution to the field.
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