{"title":"PALS-14 promotes resistance to <i>Nematocida parisii</i> infection in <i>Caenorhabditis elegans</i>.","authors":"Deevya Raman, Nicole Wernet, Spencer Gang, Emily Troemel","doi":"10.17912/micropub.biology.001325","DOIUrl":null,"url":null,"abstract":"<p><p>Microsporidia are common natural pathogens of the nematode <i>Caenorhabditis elegans</i> . Infection of <i>C. elegans</i> by the microsporidian species <i>Nematocida parisii</i> leads to induction of the Intracellular Pathogen Response (IPR), including transcriptional upregulation of 26 <i>pals</i> genes. The divergent ' <i>pals</i> ' sequence signature is conserved with humans, but PALS proteins have unknown biochemical functions. So far, none of the 26 induced <i>pals</i> genes have a demonstrated role in immunity. Here, we use RNAseq data, RNA interference, and CRISPR/Cas9 mutant analysis to identify the <i>N. parisii</i> -induced <i>pals-14</i> gene as an immune gene that provides defense against microsporidia infection in <i>C. elegans</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519727/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001325","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Microsporidia are common natural pathogens of the nematode Caenorhabditis elegans . Infection of C. elegans by the microsporidian species Nematocida parisii leads to induction of the Intracellular Pathogen Response (IPR), including transcriptional upregulation of 26 pals genes. The divergent ' pals ' sequence signature is conserved with humans, but PALS proteins have unknown biochemical functions. So far, none of the 26 induced pals genes have a demonstrated role in immunity. Here, we use RNAseq data, RNA interference, and CRISPR/Cas9 mutant analysis to identify the N. parisii -induced pals-14 gene as an immune gene that provides defense against microsporidia infection in C. elegans .
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