A cytoplasmic osmosensing mechanism mediated by molecular crowding–sensitive DCP5

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Science Pub Date : 2024-11-01 DOI:10.1126/science.adk9067

Zhenyu Wang, Qiuhua Yang, Dan Zhang, Yuanyi Lu, Yichuan Wang, Yajie Pan, Yuping Qiu, Yongfan Men, Wei Yan, Zhina Xiao, Ruixue Sun, Wenyang Li, Hongda Huang, Hongwei Guo

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Abstract

Plants are frequently challenged by osmotic stresses. How plant cells sense environmental osmolarity changes is not fully understood. We report that Arabidopsis Decapping 5 (DCP5) functions as a multifunctional cytoplasmic osmosensor that senses and responds to extracellular hyperosmolarity. DCP5 harbors a plant-specific intramolecular crowding sensor (ICS) that undergoes conformational change and drives phase separation in response to osmotically intensified molecular crowding. Upon hyperosmolarity exposure, DCP5 rapidly and reversibly assembles to DCP5-enriched osmotic stress granules (DOSGs), which sequestrate plenty of mRNA and regulatory proteins, and thus adaptively reprograms both the translatome and transcriptome to facilitate plant osmotic stress adaptation. Our findings uncover a cytoplasmic osmosensing mechanism mediated by DCP5 with plant-specific molecular crowding sensitivity and suggest a stress sensory function for hyperosmotically induced stress granules.

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由分子拥挤敏感性 DCP5 介导的细胞质渗透感应机制

植物经常受到渗透胁迫的挑战。植物细胞如何感知环境渗透压的变化尚未完全清楚。我们报告了拟南芥脱帽鞘 5（DCP5）作为一种多功能细胞质渗透传感器的功能，它能感知并响应细胞外的高渗透压。DCP5 内含植物特异性分子内拥挤传感器（ICS），该传感器会发生构象变化并驱动相分离，以响应渗透压增强的分子拥挤。在暴露于高渗透压时，DCP5 会快速、可逆地组装成富含 DCP5 的渗透胁迫颗粒（DOSGs），其中封存大量 mRNA 和调控蛋白，从而适应性地重新规划翻译组和转录组，促进植物对渗透胁迫的适应。我们的发现揭示了一种由DCP5介导的细胞质渗透传感机制，该机制具有植物特异的分子拥挤敏感性，并表明高渗透诱导的胁迫颗粒具有胁迫传感功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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