Recent Advances in Genetic Engineering Strategies of Sinorhizobium meliloti.

IF 3.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS ACS Synthetic Biology Pub Date : 2024-10-31 DOI:10.1021/acssynbio.4c00348

Xuan Liu, Huina Dong, Huiying Wang, Xinyi Ren, Xia Yang, Tingting Li, Gang Fu, Miaomiao Xia, Huan Fang, Guangqing Du, Zhaoxia Jin, Dawei Zhang

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Abstract

Sinorhizobium meliloti is a free-living soil Gram-negative bacterium that participates in nitrogen-fixation symbiosis with several legumes. S. meliloti has the potential to be utilized for the production of high-value nutritional compounds, such as vitamin B₁₂. Advances in gene editing tools play a vital role in the development of S. meliloti strains with enhanced characteristics for biotechnological applications. Several novel genetic engineering strategies have emerged in recent years to investigate genetic modifications in S. meliloti. This review provides a comprehensive overview of the mechanism and application of the extensively used Tn5-mediated genetic engineering strategies. Strategies based on homologous recombination and site-specific recombination were also discussed. Subsequently, the development and application of the genetic engineering strategies utilizing various CRISPR/Cas systems in S. meliloti are summarized. This review may stimulate research interest among scientists, foster studies in the application areas of S. meliloti, and serve as a reference for the utilization of genome editing tools for other Rhizobium species.

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瓜萎镰刀菌（Sinorhizobium meliloti）基因工程战略的最新进展。

美乐藻单胞菌（Sinorhizobium meliloti）是一种自由生活的土壤革兰氏阴性菌，与几种豆科植物共生固氮。S. meliloti 有潜力用于生产高价值的营养化合物，如维生素 B12。基因编辑工具的进步在开发具有更强特性的 S. meliloti 菌株用于生物技术应用方面发挥着至关重要的作用。近年来出现了几种新的基因工程策略来研究 S. meliloti 的基因修饰。本综述全面概述了广泛使用的 Tn5 介导的基因工程策略的机制和应用。还讨论了基于同源重组和位点特异性重组的策略。随后，总结了利用各种 CRISPR/Cas 系统的基因工程策略在 S. meliloti 中的发展和应用。本综述可激发科学家的研究兴趣，促进对 S. meliloti 应用领域的研究，并为其他根瘤菌物种利用基因组编辑工具提供参考。

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.