The transcription factor MfbHLH104 from Myrothamnus flabellifolia promotes drought tolerance of Arabidopsis thaliana by enhancing stability of the photosynthesis system.

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of biotechnology Pub Date : 2024-10-29 DOI:10.1016/j.jbiotec.2024.10.011
Zhuo Huang, Xiangying Xiang, Wenxin Xu, Li Song, Rong Tang, Duoer Chen, Qiao Li, Yujue Zhou, Cai-Zhong Jiang
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Abstract

The resurrection plant Myrothamnus flabellifolia can survive extreme drought and desiccation conditions, and quickly recover after rewatering. However, little is known about the mechanism underlying the drought tolerance of M. flabellifolia. In this study, MfbHLH104 was cloned and introduced into Arabidopsis thaliana due to the lack of a transgenic system for M. flabellifolia. MfbHLH104 is localized in the nucleus. Its N-terminal region has transactivation ability in yeast, and the C-terminal region may inhibit the transactivation ability. Overexpressing MfbHLH104 significantly increased drought and salt tolerance of A. thaliana at both seedling and adult stages. It enhanced leaf water retention capacity by decreasing water loss rate and increasing drought- and abscisic acid (ABA) -induced stomatal closure. Additionally, it boosted osmolyte accumulation and ROS scavenging ability by up-regulating genes associated with osmolyte biosynthesis and antioxidant enzymes, and enhancing antioxidant enzyme activities. The expression of ABA-responsive genes were also promoted by MfbHLH104. Remarkably, RNA-seq analysis indicated that MfbHLH104 significantly up-regulated 32 genes (FDR < 0.05 and fold change ≥1.5) involved in photosynthesis related pathways (KEGG pathway No: ko00195, ko00196) under drought, which account for 18.7 % of the total up-regulated genes and the most enriched KEGG pathways. This result suggested that it may help to maintain the stability of the photosynthesis system under drought conditions.

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拟南芥转录因子MfbHLH104通过提高光合作用系统的稳定性促进拟南芥的耐旱性。
复活植物花叶蕨(Myrothamnus flabellifolia)能在极端干旱和干燥条件下存活,并在重新浇水后迅速恢复。然而,人们对M. flabellifolia的耐旱机制知之甚少。由于缺乏拟南芥的转基因系统,本研究克隆了 MfbHLH104 并将其导入拟南芥。MfbHLH104 定位于细胞核中。其 N 端区域在酵母中具有反式激活能力,而 C 端区域可能会抑制反式激活能力。过表达 MfbHLH104 能显著提高黄连木幼苗期和成株期的耐旱性和耐盐性。它通过降低失水率和增加干旱和脱落酸(ABA)诱导的气孔关闭来提高叶片的保水能力。此外,它还通过上调与渗透溶质生物合成和抗氧化酶相关的基因以及提高抗氧化酶活性,促进渗透溶质的积累和清除 ROS 的能力。MfbHLH104 还促进了 ABA 响应基因的表达。值得注意的是,RNA-seq分析表明,MfbHLH104显著上调了干旱条件下光合作用相关通路(KEGG通路编号:ko00195、ko00196)的32个基因(FDR<0.05,折合变化率≥1.5),占上调基因总数的18.7%,是KEGG通路中富集最多的通路。这一结果表明,它可能有助于维持干旱条件下光合作用系统的稳定性。
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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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