Dose and time dependent morphodynamic changes in the ovary of nano-nickel treated rats A SEM study

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-29 DOI:10.1016/j.tice.2024.102598

Meenu Singh, Yeshvandra Verma, SV S. Rana

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Abstract

Aims

Present study demonstrates dose and time dependent effects of NiONPs (<30 nm) on the ovaries of Wistar rat.

Methods

Female rats were gavaged NiONPs or NiOMPs (5 mg/kg b.w.) for 24 h, 15 days and 30 days, euthanized and ovaries thus removed were analyzed for nickel bioaconcentration and processed for scanning electron microscopy. Serum samples were analyzed to compare the effects of nickel nano & microparticles on progesterone and estradiol values.

Results

Results confirmed the bioaccumulation of Ni in ovarian tissue. Its concentration was higher in NiONPs treated rats than NiOMPs treated rats. Progesterone level increased whereas estradiol values decreased in NiONPs and NiOMPs treated rats. SEM results also exhibited dose dependent effects on the morphology of corpoluteal complex. The structural changes varied from formation of blebs to distorted microvilli and germinal epithelium.

Conclusion

It is hypothesized that NiONPs/NiOMPs are biodegraded into smaller fragments that conjugate with amino acids and or alter downstream signaling pathways, generate ROS and modulate protein structure activity relationships. Finally, these processes manifest into morphological alterations in the ovary. Biopersistence of nickel in female reproductive system may compromise with fertility and reproductive performance of exposed population.

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纳米镍处理过的大鼠卵巢中与剂量和时间相关的形态变化 SEM 研究

方法分别给雌性大鼠灌胃NiONPs或NiOMPs（5毫克/千克体重）24小时、15天和30天，然后安乐死，取出卵巢分析镍的生物累积浓度并进行扫描电子显微镜检查。对血清样本进行分析，以比较纳米镍& 微颗粒对孕酮和雌二醇值的影响。结果证实了镍在卵巢组织中的生物蓄积性，镍纳米微粒处理大鼠的镍浓度高于镍氧化微粒处理大鼠。NiONPs和NiOMPs处理大鼠的孕酮水平升高，而雌二醇值降低。扫描电子显微镜（SEM）结果也显示了剂量对冠突复合体形态的影响。结论据推测，NiONPs/NiOMPs 被生物降解成更小的片段，这些片段与氨基酸结合，或改变下游信号通路，产生 ROS 并调节蛋白质结构和活性关系。最后，这些过程表现为卵巢的形态改变。镍在女性生殖系统中的生物持久性可能会影响接触人群的生育能力和生殖能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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