Evaluating the involvement and mutual interaction of wbp2 and yap in embryogenesis with an emphasis on liver function in zebrafish embryos

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-29 DOI:10.1016/j.tice.2024.102600
Nikita Lykov , Huiling Wang , Mogellah John Panga, Zhanxiang Du, Ziyi Chen, Shitian Chen, Lin Zhu, Ye Zhao
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Abstract

The Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) play complex roles in liver health, influencing processes such as fibrosis, cancer development, and regeneration. WW domain binding protein-2 (WBP2) primarily enhances the co-translational activity of YAP/TAZ, which is crucial for the progression of liver diseases. Despite existing knowledge, the specific functions of WBP2 and its interactions with YAP remain inadequately understood. This study investigates the expression levels of WBP2 in zebrafish embryos and its molecular interaction with YAP. We employed morpholino-mediated knockdown of wbp2 and yap, followed by assessments of liver histology, immunofluorescence, and co-immunoprecipitation. Subsequently, RNA sequencing analyses were conducted to elucidate the signaling pathways and mechanisms underlying the interplay between YAP and WBP2 in liver injury. Our findings highlight the significant interaction between WBP2 and YAP, emphasizing their potential as therapeutic targets for liver diseases.
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以斑马鱼胚胎的肝功能为重点,评估 wbp2 和 yap 在胚胎发育过程中的参与和相互作用
Yes相关蛋白(YAP)和具有PDZ结合基调的转录辅激活因子(TAZ)在肝脏健康中发挥着复杂的作用,影响着肝纤维化、癌症发展和再生等过程。WW结构域结合蛋白-2(WBP2)主要增强YAP/TAZ的共翻译活性,这对肝脏疾病的进展至关重要。尽管已有相关知识,但人们对 WBP2 的具体功能及其与 YAP 的相互作用仍不甚了解。本研究调查了 WBP2 在斑马鱼胚胎中的表达水平及其与 YAP 的分子相互作用。我们采用吗啉诺介导的方法敲除了 WBP2 和 YAP,然后对肝脏组织学、免疫荧光和共免疫沉淀进行了评估。随后进行了 RNA 测序分析,以阐明 YAP 和 WBP2 在肝损伤中相互作用的信号通路和机制。我们的研究结果突显了 WBP2 和 YAP 之间的重要相互作用,强调了它们作为肝病治疗靶点的潜力。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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