Aptamer-functionalized gold nanoparticles for fast and selective electrochemical sensing of lead in tobacco

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-10-29 DOI:10.1016/j.ijoes.2024.100858

Liang Zhang , Jinming Wu , Mingli Xiao , Shuo Zhang , Shengchao Ren , Dongsheng Luo , Feihu Xi , Huan Liu , Yaoguang Li , Quansheng Li , Yanqiu Jing

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Abstract

This study presents a novel electrochemical aptasensor for the ultrasensitive detection of lead(II) ions in tobacco products. The sensor utilizes a G-quadruplex forming DNA aptamer (5′-GGGTGGGTGGGTGGGT-3′) immobilized on gold nanoparticles (AuNPs) with an average diameter of 73.2 nm. The aptamer-Pb²⁺ interaction, characterized by circular dichroism and isothermal titration calorimetry, revealed a dissociation constant of 0.28 ± 0.03 μM. Optimized sensor fabrication conditions included 1 μM aptamer concentration and 16-h incubation time. The aptasensor exhibited two linear ranges: 0.2072–103.6 μg/L and 103.6–1072 μg/L, with a detection limit of 37 ng/L. The sensor maintained selectivity in the presence of interfering ions at 100-fold higher concentrations. The sensor demonstrated excellent stability, retaining 92.1 % of its initial response after 30 days, and high reproducibility with an inter-batch RSD of 5.3 %. This aptasensor offers a promising platform for rapid and sensitive Pb²⁺ detection in complex matrices.

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用于快速、选择性地电化学检测烟草中铅含量的 Aptamer 功能化金纳米粒子

本研究提出了一种用于超灵敏检测烟草制品中铅（II）离子的新型电化学适配体传感器。该传感器利用固定在平均直径为 73.2 nm 的金纳米粒子（AuNPs）上的 G-四联体 DNA 合体（5′-GGGTGGGTGGGTGGGT-3′）。通过圆二色性和等温滴定量热法表征的aptamer-Pb2+相互作用显示，其解离常数为 0.28 ± 0.03 μM。优化的传感器制造条件包括 1 μM 的适配体浓度和 16 小时的孵育时间。该适配体传感器有两个线性范围：0.2072-103.6 μg/L 和 103.6-1072 μg/L，检测限为 37 ng/L。在干扰离子浓度高出 100 倍的情况下，该传感器仍能保持选择性。该传感器具有极佳的稳定性，30 天后仍能保持 92.1% 的初始响应，而且重现性很高，批间 RSD 为 5.3%。这种适配传感器为在复杂基质中快速灵敏地检测 Pb2+ 提供了一个前景广阔的平台。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.