Evaluation of Guanidinoacetic Acid Supplementation on Finishing Beef Steer Growth Performance, Skeletal Muscle Cellular Response, and Carcass Characteristics

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of animal science Pub Date : 2024-11-01 DOI:10.1093/jas/skae337

Erika P Eckhardt, Wonseob Kim, Jerad Jaborek, Andrea J Garmyn, Donghun Kang, Jongkyoo Kim

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Abstract

This study elucidated the effects of dosage-dependent guanidinoacetic acid (GAA) supplementation on growth performance, muscle responses, and carcass characteristics in finishing beef steers. Thirty crossbred Red Angus beef steers (395 ± 28.09 kg) were randomly assigned one of three treatments during a 146-day feedlot study: basal diet without GAA supplementation (CONTROL), 1g of GAA per 100 kg of BW daily (LOWGAA), and 2g of GAA per 100 kg of BW daily (HIGHGAA). Individual feed intake was monitored daily, growth performance parameters were collected every 28 days, and longissimus muscle (LM) biopsies occurred every 56 days. In biopsied LM, greater (P = 0.048) mRNA expression of IGF-1 was observed in LOWGAA steers on d 112 compared to the CONTROL group. LOWGAA steers also exhibited greater expression of myosin heavy chain (MHC) I compared to CONTROL steers (P < 0.05) and MHC IIA compared to both CONTROL and HIGHGAA treatment groups (P < 0.01) on d 112. GAA supplementation resulted in no change in carcass characteristics, serum and LM tissue metabolites, LM composition, and Warner-Bratzler shear force (WBSF) values (P > 0.05). Data collected from this study demonstrate the influence of GAA supplementation on the gene expression of MHC isoforms and their role in skeletal muscle growth, differentiation, and muscle fiber-typing.

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评估添加胍基乙酸对育成肉牛生长性能、骨骼肌细胞反应和胴体特征的影响

本研究阐明了剂量依赖性鸟苷酸（GAA）补充剂对育成肉牛生长性能、肌肉反应和胴体特征的影响。在为期 146 天的饲养研究中，30 头杂交红安格斯肉牛（395 ± 28.09 千克）被随机分配到三种处理之一：不添加 GAA 的基础日粮（对照组）、每天每 100 千克体重添加 1 克 GAA（低 GAA）和每天每 100 千克体重添加 2 克 GAA（高 GAA）。每天监测个体采食量，每 28 天收集一次生长性能参数，每 56 天进行一次长肌（LM）活检。与对照组相比，LOWGAA 牛在第 112 天的长肌肉活检中观察到更高的（P = 0.048）IGF-1 mRNA 表达量。在第 112 天，与对照组相比，LOWGAA 牛的肌球蛋白重链 (MHC) I 表达量更高（P&;lt; 0.05），与对照组和 HIGHGAA 处理组相比，MHC IIA 表达量更高（P&;lt; 0.01）。补充 GAA 不会导致胴体特征、血清和 LM 组织代谢物、LM 成分和 Warner-Bratzler 剪切力 (WBSF) 值发生变化（P &;gt;0.05）。本研究收集的数据证明了补充 GAA 对 MHC 同工酶基因表达的影响及其在骨骼肌生长、分化和肌纤维类型中的作用。

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.