Unique structural attributes of the PSI-NDH supercomplex in Physcomitrium patens.

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-03 DOI:10.1111/tpj.17116
Monika Opatíková, Roman Kouřil
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Abstract

Cyclic electron transport around photosystem I (PSI) is essential for the protection of the photosynthetic apparatus in plants under diverse light conditions. This process is primarily mediated by Proton Gradient Regulation 5 protein/Proton Gradient Regulation 5-like photosynthetic phenotype 1 protein (PGR5/PGRL1) and NADH dehydrogenase-like complex (NDH). In angiosperms, NDH interacts with two PSI complexes through distinct monomeric antennae, LHCA5 and LHCA6, which is crucial for its higher stability under variable light conditions. This interaction represents an advanced evolutionary stage and offers limited insight into the origin of the PSI-NDH supercomplex in evolutionarily older organisms. In contrast, the moss Physcomitrium patens (Pp), which retains the lhca5 gene but lacks the lhca6, offers a glimpse into an earlier evolutionary stage of the PSI-NDH supercomplex. Here we present structural evidence of the Pp PSI-NDH supercomplex formation by single particle electron microscopy, demonstrating the unique ability of Pp to bind a single PSI in two different configurations. One configuration closely resembles the angiosperm model, whereas the other exhibits a novel PSI orientation, rotated clockwise. This structural flexibility in Pp is presumably enabled by the variable incorporation of LHCA5 within PSI and is indicative of an early evolutionary adaptation that allowed for greater diversity at the PSI-NDH interface. Our findings suggest that this variability was reduced as the structural complexity of the NDH complex increased in vascular plants, primarily angiosperms. This study not only clarifies the evolutionary development of PSI-NDH supercomplexes but also highlights the dynamic nature of the adaptive mechanisms of plant photosynthesis.

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专利植物中 PSI-NDH 超级复合物的独特结构属性。
在不同的光照条件下,围绕光系统 I(PSI)的循环电子传递对于保护植物的光合装置至关重要。这一过程主要由质子梯度调节 5 蛋白/质子梯度调节 5 样光合表型 1 蛋白(PGR5/PGRL1)和 NADH 脱氢酶样复合物(NDH)介导。在被子植物中,NDH 通过不同的单体触角 LHCA5 和 LHCA6 与两个 PSI 复合物相互作用,这对其在多变光照条件下保持较高的稳定性至关重要。这种相互作用代表了一个高级进化阶段,对更古老生物体中 PSI-NDH 超级复合物的起源提供了有限的启示。相比之下,保留了 lhca5 基因但缺乏 lhca6 基因的藓类 Physcomitrium patens(Pp)则为 PSI-NDH 超级复合物的早期进化阶段提供了一瞥。在这里,我们通过单颗粒电子显微镜展示了 Pp PSI-NDH 超级复合物形成的结构证据,证明了 Pp 以两种不同构型结合单个 PSI 的独特能力。一种构型与被子植物模型非常相似,而另一种构型则表现出一种顺时针旋转的新型 PSI 取向。Pp的这种结构灵活性可能是通过LHCA5在PSI中的可变结合实现的,并表明早期的进化适应使PSI-NDH界面具有更大的多样性。我们的研究结果表明,随着维管植物(主要是被子植物)中 NDH 复合物结构复杂性的增加,这种可变性有所降低。这项研究不仅阐明了 PSI-NDH 超级复合体的进化发展,还突出了植物光合作用适应机制的动态性质。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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