OsBRW1, a novel blast-resistant gene, coded a NBS-LRR protein to interact with OsSRFP1 to balance rice growth and resistance.

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Journal Pub Date : 2024-11-03 DOI:10.1111/pbi.14494
Shiwei Ma, Shichang Xu, Huan Tao, Yunxia Huang, Changqing Feng, Guanpeng Huang, Shoukai Lin, Yiqiong Sun, Xuan Chen, Manegdebwaoga Arthur Fabrice Kabore, Samuel Tareke Woldegiorgis, Yufang Ai, Lina Zhang, Wei Liu, Huaqin He
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Abstract

It is urgent to mine novel blast-resistant genes in rice and develop new rice varieties with pyramiding blast-resistant genes. In this study, a new blast-resistant gene, OsBRW1, was screened from a set of rice near-isogenic lines (NILs) with different blast-resistant ability. Under the infection of Magnaporthe oryzae (M. oryzae), OsBRW1 in the resistant NIL Pi-4b was highly induced than that in the susceptible NIL Pi-1 and their parent line CO39, and the blast-resistant ability of OsBRW1 was further confirmed by using CRISPR/Cas9 knockout and over-expression methods. The protein encoded by OsBRW1 was a typical NBS-LRR with NB-ARC domain and localized in both cytoplasm and nucleus, and the transient expression of OsBRW1 was capable of triggering hypersensitive response in tobacco leaves. Protein interaction experiments showed that OsBRW1 protein directly interacted with OsSRFP1. At the early infection stage of M. oryzae, OsBRW1 gene induced OsSRFP1 to highly expression level and accumulated H2O2, up-regulated the defence responsive signalling transduction genes and the pathogenesis-related genes and increased JA and SA content in the resistant NIL Pi-4b. By contrary, lower content of endogenous JA and SA in osbrw1 mutants was found at the same stage. After that, OsSRFP1 was down-regulated to constitution abundance to balance the growth of the resistant NIL Pi-4b. In summary, OsBRW1 solicited OsSRFP1 to resist the infection of blast fungus in rice by inducing the synergism of induced systemic resistance (ISR) and system acquired resistance (SAR) and to balance the growth of rice plants.

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OsBRW1 是一种新型抗瘟基因,编码一种 NBS-LRR 蛋白,与 OsSRFP1 相互作用,平衡水稻的生长和抗性。
挖掘水稻中的新型抗瘟基因,培育具有金字塔抗瘟基因的水稻新品种迫在眉睫。本研究从一组具有不同抗瘟能力的水稻近等基因系(NILs)中筛选出了一个新的抗瘟基因OsBRW1。在Magnaporthe oryzae(M. oryzae)的感染下,抗性NIL Pi-4b中的OsBRW1比易感NIL Pi-1及其亲本品系CO39中的OsBRW1具有更高的诱导性,并通过CRISPR/Cas9基因敲除和过表达的方法进一步证实了OsBRW1的抗瘟能力。OsBRW1编码的蛋白质是一种典型的NBS-LRR,具有NB-ARC结构域,定位于细胞质和细胞核中,瞬时表达OsBRW1能引发烟草叶片的超敏反应。蛋白质相互作用实验表明,OsBRW1 蛋白与 OsSRFP1 直接相互作用。在M. oryzae感染初期,OsBRW1基因诱导OsSRFP1达到高表达水平并积累H2O2,上调抗性NIL Pi-4b的防御反应信号转导基因和致病相关基因,增加JA和SA含量。相反,在同一阶段,osbrw1 突变体的内源 JA 和 SA 含量较低。之后,OsSRFP1被下调至构成丰度,以平衡抗性NIL Pi-4b的生长。综上所述,OsBRW1通过诱导系统抗性(ISR)和系统获得性抗性(SAR)的协同作用,促使OsSRFP1抵抗稻瘟病真菌的侵染,并平衡水稻植株的生长。
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来源期刊
Plant Biotechnology Journal
Plant Biotechnology Journal 生物-生物工程与应用微生物
CiteScore
20.50
自引率
2.90%
发文量
201
审稿时长
1 months
期刊介绍: Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.
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