E. coli-Assisted Eco-Friendly Production of Biogenic Silver Cobalt Oxide (AgCoO2) Nanoparticles: Methanolysis-Based Hydrogen Production, Wastewater Remediation, and Pathogen Control.

IF 2 3区 工程技术 Q2 ANATOMY & MORPHOLOGY Microscopy Research and Technique Pub Date : 2024-11-04 DOI:10.1002/jemt.24718
Aisha Irshad, Sajid Mahmood, Tanzeela Fazal, Shahid Iqbal, Mujaddad-Ur- Rehman, Ammar Zidan, Mazloom Shah, Ali Bahadur, Azam Hayat, Asma Khan, Attiya Abdul Malik, Nasser S Awwad, Hala A Ibrahium
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Abstract

Herein, bacterial-assisted synthesis of AgCoO2 is carried out. In the first step, E. coli was separated from soil samples via the "serial dilution method." Ten milliliters of bacterial supernatant was mixed with cobalt chloride and silver nitrate hatched at 38°C for 24 h to get AgCoO2 nanoparticles (NPs). XRD results confirm the synthesis of AgCoO2 NPs while EDX results confirm the absence of any other elements than Ag, Co, and O. An average NP size of 12-26 nm was determined by TEM examination, and the surface of the particles was seen rough, irregularly shaped borders. The antibacterial activity of the constructed NPs was checked against S. aureus, E. coli, Bacillus subtilus, and Pseudomanas areguinosa using agar well diffusion method. The maximum zone of inhibition was 27 mm at 40 mg/mL against Bacillus subtilus. The performance of the synthesized NPs as photocatalysts was also assessed, and several operational parameters that control the photodegradation of the harmful dyes were tried to tune as well, and 85% degrading efficiency was obtained at 60oC for 240 min for 30 mg of catalyst dose These NPs were also used to produce hydrogen by methanolysis.

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大肠杆菌辅助生态友好型生物银氧化钴(AgCoO2)纳米颗粒的生产:基于甲醇分解的氢气生产、废水修复和病原体控制。
在此,我们进行了细菌辅助合成 AgCoO2 的研究。第一步,通过 "连续稀释法 "从土壤样本中分离出大肠杆菌。将 10 毫升细菌上清液与氯化钴和硝酸银混合,在 38°C 孵育 24 小时,得到 AgCoO2 纳米粒子(NPs)。XRD 结果证实了 AgCoO2 NPs 的合成,而 EDX 结果则证实了除 Ag、Co 和 O 外不存在任何其他元素。采用琼脂井扩散法检测了所构建的 NPs 对金黄色葡萄球菌、大肠杆菌、枯草杆菌和假丝酵母菌的抗菌活性。在 40 毫克/毫升的浓度下,对枯草芽孢杆菌的最大抑菌区为 27 毫米。此外,还对合成的 NPs 作为光催化剂的性能进行了评估,并尝试调整控制有害染料光降解的几个操作参数,结果表明,在 60oC 240 分钟、催化剂剂量为 30 毫克的条件下,降解效率为 85%。
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来源期刊
Microscopy Research and Technique
Microscopy Research and Technique 医学-解剖学与形态学
CiteScore
5.30
自引率
20.00%
发文量
233
审稿时长
4.7 months
期刊介绍: Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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