A fish intestinal in vitro model for investigation of lipid metabolism and steatosis

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-10-28 DOI:10.1016/j.bbalip.2024.159573
Daphne Siciliani , Bente Ruyter , Guro Løkka , Kirsti Elisabeth Præsteng , Matteo Minghetti , Trond M. Kortner
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Abstract

Choline is now recognized as an essential nutrient to ensure lipid transport in Atlantic salmon. Its deficiency leads to excessive lipid accumulation in the enterocytes, a condition known as steatosis. The knowledge of lipid metabolism and steatosis in fish remains limited, motivating the use of in vitro intestinal models to perform deeper explorations. This study aimed to create an in vitro steatosis model using RTdi-MI, a new cell line derived from the distal intestine of rainbow trout. Cells were exposed to varying oleic acid (OA) concentrations over different time points (24 h, 72 h, and 168 h). Results indicated that the increasing OA concentration enhanced intracellular lipid droplet formation. Quantitative lipid analysis confirmed OA accumulation, which intensified with prolonged exposure and increased OA dose. Moreover, all cells, including controls, exhibited fatty acid metabolic activity. Such outcome was confirmed by light and fluorescence microscopy. Additionally, RTdi-MI cells expressed genes involved in lipid metabolism and synthesis similar to in vivo conditions. Collectively, our findings demonstrate the ability of RTdi-MI cells to accumulate OA in intracellular lipid droplets and mirror in vivo steatosis conditions, offering a new tool for exploring fish intestinal lipid metabolism.
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用于研究脂质代谢和脂肪变性的鱼肠体外模型。
胆碱是目前公认的确保大西洋鲑脂质运输的必需营养素。缺乏胆碱会导致肠细胞中脂质过度积累,这种情况被称为脂肪变性。对鱼类脂质代谢和脂肪变性的了解仍然有限,这促使人们使用体外肠道模型进行更深入的探索。本研究旨在利用 RTdi-MI(一种源自虹鳟鱼远端肠道的新细胞系)创建体外脂肪变性模型。细胞在不同的时间点(24 小时、72 小时和 168 小时)暴露于不同浓度的油酸(OA)中。结果表明,OA 浓度的增加会促进细胞内脂滴的形成。定量脂质分析证实了 OA 的积累,这种积累随着暴露时间的延长和 OA 剂量的增加而加剧。此外,包括对照组在内的所有细胞都表现出脂肪酸代谢活性。光镜和荧光显微镜证实了这一结果。此外,RTdi-MI 细胞表达的参与脂质代谢和合成的基因与体内情况类似。总之,我们的研究结果表明 RTdii-MI 细胞能够在细胞内脂滴中积累 OA,并反映体内脂肪变性的情况,为探索鱼类肠道脂质代谢提供了一种新工具。
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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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