Single-cell transcriptomics and tissue metabolomics uncover mechanisms underlying wooden breast disease in broilers

IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Poultry Science Pub Date : 2024-10-19 DOI:10.1016/j.psj.2024.104433
Di Zhao , Zehe Song , Li Shen , Tian Xia , Qingyuan Ouyang , Haihan Zhang , Xi He , Kelang Kang
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Abstract

Accompanied by the accelerated growth rate of chickens, the quality of chicken meat has deteriorated in recent years. Wooden breast (WB) is a severe myopathy affecting meat quality, and its pathophysiology depends on gene expression and intercellular interactions of various cell types, which are not yet fully understood. We have performed a comprehensive transcriptomic and metabolomic atlas of chicken WB muscle. Our data showed a significant increase in the number of immune cells, WB muscle displayed a unique cluster of macrophages (cluster 11), distinct from the M1 and M2 macrophages. Regarding the myocytes, the most significant differences were the decrease in cell number and the intensification of fatty deposits. Satellite cells were involved in muscle repair and regeneration producing more collagen. Interestingly, the interaction network in the WB group was weaker compared to that in normal breast muscle. Additionally, we found six key differential metabolites across 22 pathways. When WB occurs, myocytes and endothelial cells undergo apoptosis, macrophages are activated and exert immune functions, satellite cells participate in muscle rebuilding and repair, and the content of metabolites undergoes significant changes. This cell transcriptome profile provides an essential reference for future studies on the development and remodeling of WB.
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单细胞转录组学和组织代谢组学揭示肉鸡木质乳房疾病的内在机制
近年来,随着鸡只生长速度的加快,鸡肉的质量也在不断下降。木质胸肌(WB)是一种影响肉质的严重肌病,其病理生理学取决于各种细胞类型的基因表达和细胞间相互作用,而目前对这些尚未完全了解。我们对鸡肉板筋肌肉进行了全面的转录组和代谢组图谱研究。我们的数据显示,免疫细胞的数量明显增加,WB 肌肉显示出独特的巨噬细胞群(群 11),有别于 M1 和 M2 巨噬细胞。在肌细胞方面,最显著的差异是细胞数量的减少和脂肪沉积的增加。卫星细胞参与肌肉修复和再生,产生更多胶原蛋白。有趣的是,与正常乳腺肌肉相比,WB 组的相互作用网络更弱。此外,我们还在 22 条途径中发现了六种关键的差异代谢物。发生 WB 时,肌细胞和内皮细胞会凋亡,巨噬细胞会被激活并发挥免疫功能,卫星细胞会参与肌肉重建和修复,代谢物的含量也会发生显著变化。该细胞转录组图谱为今后研究 WB 的发展和重塑提供了重要参考。
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来源期刊
Poultry Science
Poultry Science 农林科学-奶制品与动物科学
CiteScore
7.60
自引率
15.90%
发文量
0
审稿时长
94 days
期刊介绍: First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
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