Poultry Science最新文献

{"title":"Transcriptome and chromatin accessibility landscape of ovarian development at different egg-laying stages in taihe black-bone silky fowls","authors":"Jingyi Zhang ,&nbsp;Yong Cui ,&nbsp;Jiming Ruan,&nbsp;Haiyan Zhu,&nbsp;Haiping Liang,&nbsp;Ji Cao,&nbsp;Qing Wei,&nbsp;Jianzhen Huang","doi":"10.1016/j.psj.2025.104864","DOIUrl":"10.1016/j.psj.2025.104864","url":null,"abstract":"<div><div>Taihe Black-Bone Silky Fowl <strong>(SF)</strong> is a famous local breed in China, known for its high nutritional and medicinal value. However, its low egg-laying rate significantly limits its economic benefits. This study aims to explore the ovarian development status, as well as the changes in the transcriptome and chromatin accessibility landscape at different egg-laying stages of SF, in order to reveal the epigenetic regulatory mechanisms underlying ovarian development in laying hens. The results showed that during peak egg-laying, serum levels of follicle stimulating hormone <strong>(FSH)</strong>, luteinizing hormone <strong>(LH)</strong>, estradiol <strong>(E2)</strong>, and progesterone <strong>(P4)</strong> in the SF were higher than in the other laying periods. Meanwhile, the serum and ovarian matrix total antioxidant capacity <strong>(T-AOC)</strong> level decreased with increasing age, whereas the ovarian matrix malondialdehyde <strong>(MDA)</strong> level showed the opposite trend. Compared to the late laying period, several genes related to ovarian development and reproductive hormone secretion, including <em>TDRD5, CCNO, CYP17A1, BMP15</em>, and <em>STAR</em>, were upregulated during the peak egg-laying period. Additionally, we identified key transcription factors <strong>(TF)</strong> associated with different egg-laying periods. Specific TF, such as Fli1, Etv2, and AT2G15740, linked to the peak egg-laying period, play significant roles in cell and tissue development. The specific transcription factor Nr5a2, associated with the late laying period, has been shown to inhibit E2 production. Furthermore, genes related to poultry reproductive performance, such as <em>STAR</em> and <em>WNT4</em>, were found to be regulated by specific distal enhancers in open chromatin regions <strong>(OCR)</strong>. In conclusion, this study elucidated the dynamic changes in the transcriptome and chromatin accessibility landscape during ovarian development in SF at different egg-laying stages and highlighted key TF, including Fli1, Etv2, and Nr5a2, as well as essential genes like <em>STAR</em> and <em>WNT4</em> that regulate ovarian development. These findings provide valuable insights into the regulatory mechanisms influencing egg-laying performance in SF and offer new strategies for improving ovarian follicle development and egg production performance in poultry.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104864"},"PeriodicalIF":3.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143219412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Pulsed electric field (PEF) processing of microalga Chlorella vulgaris and its digestibility in broiler feed” [Poultry Science, Volume 103, Issue 6, June 2024, 103721]","authors":"Sofie Van Nerom ,&nbsp;Kobe Buyse ,&nbsp;Filip Van Immerseel ,&nbsp;Johan Robbens ,&nbsp;Evelyne Delezie","doi":"10.1016/j.psj.2025.104861","DOIUrl":"10.1016/j.psj.2025.104861","url":null,"abstract":"","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104861"},"PeriodicalIF":3.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143219414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Impact of adding zeolite to broilers' diet and litter on growth, blood parameters, immunity, and ammonia emission” [Poultry Science, Volume 103, Issue 9, September 2024, 103981]","authors":"Ahmed I. Elsherbeni ,&nbsp;Islam M. Youssef ,&nbsp;Mahmoud Kamal ,&nbsp;Mai A.M. Youssif ,&nbsp;Gaafar M. El-Gendi ,&nbsp;Osama H. El-Garhi ,&nbsp;Haifa E. Alfassam ,&nbsp;Hassan A. Rudayni ,&nbsp;Ahmed A. Allam ,&nbsp;Mahmoud Moustafa ,&nbsp;Mohammed O. Alshaharni ,&nbsp;Mohammed Al-Shehri ,&nbsp;Mohamed S. El Kholy ,&nbsp;Reda E. Hamouda","doi":"10.1016/j.psj.2025.104845","DOIUrl":"10.1016/j.psj.2025.104845","url":null,"abstract":"","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104845"},"PeriodicalIF":3.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research note: Mite infestations in non-descriptive indigenous chickens in Bangladesh: Present status and pathology","authors":"Nusrat Nadia ,&nbsp;Nusrat Nowrin Shohana ,&nbsp;Md. Shahadat Hossain ,&nbsp;Sharmin Shahid Labony ,&nbsp;Md. Haydar Ali ,&nbsp;Md. Asaduzzaman Lovelu ,&nbsp;Akter Shirin ,&nbsp;Sumaiya Naznin Ritu ,&nbsp;Umme Razia Islam ,&nbsp;Romana Parvin ,&nbsp;Md. Hasanuzzaman Talukder ,&nbsp;Md. Mahmudul Alam ,&nbsp;Md. Abdul Alim ,&nbsp;Anisuzzaman","doi":"10.1016/j.psj.2025.104889","DOIUrl":"10.1016/j.psj.2025.104889","url":null,"abstract":"<div><div>Non-descriptive, indigenous chickens (<strong>NDICs</strong>) in Bangladesh are commonly reared in scavenging system. Here, we describe the prevalence and pathological impacts induced by mite infestations in NDICs. We found that 47.4 % of NDICs (185 out of 390) were infested with three species of mites namely <em>Trombicula</em> spp<em>.</em> (53.1 %), <em>Knemidocoptes mutans</em> (scaly leg mites/<strong>SLM</strong>, 15.1 %) and <em>Dermanyssus gallinae</em> (red mites of poultry/<strong>PRM</strong>, 2.8 %). Sex, age and seasons significantly (<em>p</em> &lt; 0.05) influenced the prevalence of mite infestations. Trombiculid mites produced more specific lesions characterized by circumscribed disc-shaped notches with attached bright red colored mites, which were commonly found in breast, fluff and thigh. Destruction of the epidermis, proliferation of fibrous connective tissues along with massive infiltration of heterophils and lymphocytes were predominant. Thick, rough and ragged appearances with encrustation of the skin of leg were found grossly in SLM infestations. Histologically, hyperkeratosis, acanthosis, massive infiltrations of reactive cells and cross-sections of parasites were detected. PRM induced petechial hemorrhages, papules, necrosis, rupture of the feather follicles and scab formations. Hyperkeratosis and huge infiltration of mononuclear cells and granulocytes were evident. Taken together, mite infestation is a big challenge in profitable NDICs rearing in Bangladesh and warrants immediate attention for their control.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104889"},"PeriodicalIF":3.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of vitamin E and selenium supplementation on growth, reproductive performance, and oxidative stress in dexamethasone-stressed Japanese quail cocks","authors":"Ifeanyi Emmanuel Uzochukwu ,&nbsp;Luke Chukwudi Ali ,&nbsp;Bright Chigozie Amaefule ,&nbsp;Chisom C. Okeke ,&nbsp;Charles Onochie Osita ,&nbsp;Ndubuisi Samuel Machebe ,&nbsp;Vesela Yancheva ,&nbsp;Dóra Somogyi ,&nbsp;Krisztián Nyeste","doi":"10.1016/j.psj.2025.104888","DOIUrl":"10.1016/j.psj.2025.104888","url":null,"abstract":"<div><div>This study investigated the effects of dietary vitamin E (VE) and selenium (Se) supplementation on body weight changes, blood profile, and semen quality in Dexamethasone (DEX)-stressed Japanese quails. One hundred and five 10-week-old quail cocks were acclimated and divided into five treatment groups: negative control – G1, DEX-treated (20 mgL⁻¹ of drinking water) – G2, DEX + VE (180 mg kg diet⁻¹) – G3; DEX + Se (0.3 mg kg diet⁻¹) – G4; and DEX + VE (180 mg kg diet⁻¹) + Se (0.3 mg kg diet⁻¹) – G5. The birds received their respective treatments over 21 days, and various performance, hematological, and semen quality parameters were measured. Results indicated that DEX treatment significantly reduced weight gain (WG) and feed intake (<em>P</em> &lt; 0.05). Supplementation with VE and Se, individually and combined, ameliorated these effects, with groups G3, G4, and G5 showing similar WG to the control. Hematological analysis revealed significant increases (<em>P</em> &lt; 0.05) in packed cell volume, hemoglobin, and white blood cell count in DEX-treated groups compared to G1. Treatment did not affect blood glucose and cholesterol levels (<em>P</em> ≥ 0.05). Plasma antioxidant assays showed elevated superoxide dismutase and catalase functions and reduced malondialdehyde levels in G3, G4, and G5 compared to G2, indicating reduced oxidative stress. No marked differences were seen in the plasma glutathione peroxidase activities across groups. Sperm motility was impaired in the DEX-only group but improved (<em>P</em> &lt; 0.05) with antioxidant supplementation. In conclusion, dietary VE and Se effectively mitigated the negative impacts of DEX-induced stress on growth, antioxidant status, and spermatozoa motility in Japanese quail cocks. VE and Se supplementation could be beneficial in enhancing the welfare and productivity of poultry under stress.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104888"},"PeriodicalIF":3.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143219413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory and anti-inflammatory potential of botanicals bioactive product (PHYTO AX'CELL™) for an improvement of the well-being of laying hens at the peak of production","authors":"Alessandro Guerrini ,&nbsp;Roberta Salaroli ,&nbsp;Augusta Zannoni ,&nbsp;Giancarlo Avallone ,&nbsp;Francesca Leone ,&nbsp;Valentina Serra ,&nbsp;Giulia Quaglia ,&nbsp;Francesca del Zozzo ,&nbsp;Thibaut Chabrillat ,&nbsp;Claire Carlu ,&nbsp;Caterina Lupini ,&nbsp;Doriana Eurosia Angela Tedesco","doi":"10.1016/j.psj.2025.104882","DOIUrl":"10.1016/j.psj.2025.104882","url":null,"abstract":"<div><div>This study was designed to assess the effectiveness of an authorized commercial standardized mixture of Artepillin-C, methyl-salicylates, flavonoids and curcuminoids (PHYTO AX'CELL™) provided intermittently during 8 weeks (wk) of trial to laying hens raised in enriched cages during the peak of the production, in the reduction of inflammation status, improving the immune response, and egg quality. In this study, 764 Lohmann LSL-White hens on the first day (d) of the 26^th wk of age (T0), were randomly assigned to 2 replicated experimental groups, control and treated (n=382 each). The treatment was supplied in drinking water, as follows: 26^th to 27^th wk (T1, 14 d) first treatment administration at a dose of 1 mL/L (0.5 mL/L only the first d of the treatment); 28^th to the 31^st wk (T2, 4 wk of withdrawal period); 32^nd to 33^rd wk (T3, 14 d) second treatment at a dose of 1 mL/L, until the end of the trial (T4). At T0, mid-T3 and T4, 13 hens per group were sampled for serum biochemical analyses (metabolic profile, H/L ratio) and weighed, and 60 eggs per group were analyzed for quality parameters. At T4, 13 hens per group were sacrificed for histological investigations and gut IgA quantification. The treatment reduced the mortality rate in the treated group (0.00%) compared to the control (2.61%). An improvement in intestinal IgA production and immune reactivity in the treated hens was observed with a significant fluctuating trend of the heterophil, lymphocyte and their ratio (<em>P</em> &lt; 0.05). The egg quality was improved by the treatment, with positive effects in the Haugh unit, shell weight and thickness (<em>P</em> &lt; 0.05). A T4, significant reduction in duodenal and rectal pH was observed in the treated group (<em>P</em> &lt; 0.05), without intestinal inflammation score changes, body weight, serum biochemistry, interleukin levels, and infectious bronchitis virus titers (<em>P</em> &gt; 0.05). From the results, PHYTO AX'CELL™ improved the well-being and physical condition of laying hens raised in cages, modulating the immune system with a positive production of intestinal IgA, and egg quality parameters important for commercial purposes.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104882"},"PeriodicalIF":3.8,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143219135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail","authors":"Ewa Tomaszewska ,&nbsp;Kamil Drabik ,&nbsp;Kornel Kasperek ,&nbsp;Piotr Dobrowolski ,&nbsp;Monika Hułas-Stasiak ,&nbsp;Renata Pyz-Łukasik ,&nbsp;Waldemar Paszkiewicz ,&nbsp;Monika Ziomek ,&nbsp;Janine Donaldson ,&nbsp;Katarzyna Dobrowolska ,&nbsp;Artur Burmaczuk ,&nbsp;Justyna Batkowska ,&nbsp;Marcin B. Arciszewski ,&nbsp;Siemowit Muszyński","doi":"10.1016/j.psj.2025.104879","DOIUrl":"10.1016/j.psj.2025.104879","url":null,"abstract":"<div><div>This study investigated the effects of dietary glutamine (Gln) supplementation on the performance, carcass characteristics, blood biochemistry, intestinal morphology, and gut microbiota of laying quail over a 12-week period, with analyses conducted after 6 and 12 weeks of supplementation. Seven-week-old dual-purpose female quails at the onset of laying were assigned to diets containing 0% (control), 0.5%, 1%, or 1.5% Gln. Performance metrics included laying rate, daily feed intake, and feed efficiency. Supplementation with 0.5% Gln significantly enhanced the laying rate after 12 weeks; daily feed intake and feed efficiency varied over time but were not consistently affected by Gln. Breast muscle weight was highest in quail supplemented with 1.5% Gln after 6 weeks. Blood serum analyses showed that total protein and albumin concentrations were highest in quail receiving 0% and 1% Gln after 12 weeks, while supplementation with 0.5% Gln decreased triacylglycerol levels after 12 weeks. Glucose and creatinine levels varied with Gln supplementation and between the two time points; enzyme activities (alanine transaminase, alkaline phosphatase, and gamma-glutamyl transpeptidase) were affected by both Gln dose and length of supplementation. Intestinal morphology was significantly influenced by Gln dose and supplementation period. The longest villi were observed in quail supplemented with 1% Gln after 6 weeks, decreasing by 12 weeks. The widest villi were recorded in quail receiving 1.5% Gln after 6 weeks, with villus width decreasing over time. Crypt depth varied significantly, with the deepest crypts observed at 6 weeks in quail supplemented with 1% and 1.5% Gln, becoming shallower after 12 weeks. Microbial analysis of digesta samples revealed <em>Lactobacillaceae</em> as the predominant bacterial family, followed by <em>Enterobacteriaceae</em> and genera including <em>Streptococcus</em> and <em>Staphylococcus</em>. Overall, dietary Gln supplementation influenced performance, carcass characteristics, blood biochemical parameters, intestinal morphology, and gut microbiota composition in laying quail, with effects varying between 6 and 12 weeks of supplementation. These findings suggest that Gln supplementation has the potential to enhance quail performance and health over time.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104879"},"PeriodicalIF":3.8,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized production and antiviral activity evaluation of recombinant chicken interferon-alpha using an improved Baculovirus expression system","authors":"Yuxia Zhang,&nbsp;Wenwen Dong,&nbsp;Feng Li,&nbsp;Kai Meng,&nbsp;Guiming Li,&nbsp;Xiaoyuan Yuan","doi":"10.1016/j.psj.2025.104870","DOIUrl":"10.1016/j.psj.2025.104870","url":null,"abstract":"<div><div>Despite extensive vaccination efforts, certain pathogens continue to pose significant challenges to the poultry industry. Interferons (<strong>IFNs</strong>) are crucial for innate immune defense against various pathogens. Chicken interferon-alpha (<strong>chIFN-α</strong>) reportedly has good inhibitory activity against various pathogens. However, its production is often limited by low yields. This study aimed to enhance the production of chIFN-α using an improved baculovirus expression system and evaluate its antiviral efficacy both in vivo and in vitro. A recombinant bacmid carrying two copies of the chIFN-α gene was successfully constructed. The chIFN-α protein was obtained using the modified baculovirus expression system, and exhibited anti-vesicular stomatitis virus (<strong>VSV</strong>) activity of up to 5.0 × 10⁶ IU/ml. Moreover, the administration of chIFN-α to SPF chicken inhibited H9N2 subtype low pathogenicity avian influenza virus (<strong>LPAIV</strong>) proliferation and reduced the viral-shedding rate. In the present study, we successfully expressed and purified chIFN-α at high yields, as well as demonstrated its strong antiviral activity in vivo and in vitro. These findings present an alternative approach for the efficient production of chIFN-α for therapeutic applications.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104870"},"PeriodicalIF":3.8,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EGCG improve meat quality, restore lipid metabolism disorder and regulate intestinal flora in high-fat fed broilers","authors":"Lujia Gao,&nbsp;Chen Liu,&nbsp;Jiaqi Wu,&nbsp;Ying Cui,&nbsp;Man Zhang,&nbsp;Chongpeng Bi,&nbsp;Anshan Shan,&nbsp;Xiujing Dou","doi":"10.1016/j.psj.2025.104875","DOIUrl":"10.1016/j.psj.2025.104875","url":null,"abstract":"<div><div>Excessive oil addition can easily result in decreased disease resistance in broilers, a drop in meat quality, and disorders of glucose and lipid metabolism. Epigallocatechin gallate (EGCG) is an important bioactive component of tea and has been shown to have promising effects on the metabolism of nutrients. This study was aimed at investigating the impact of EGCG supplementation through a high-fat diet (HFD) on production performance, meat quality, lipid metabolism and the influence of intestinal flora in broiler chickens. During the experimental phase, the broilers were segregated into three groups and provided with distinct diets: a basal diet, a high-fat diet, and a high-fat diet supplemented with EGCG, respectively. The results showed that EGCG increased lightness (L*) 24 h (<em>P</em> &lt; 0.05), and decreased drip loss (<em>P</em> &lt; 0.05) of chicken meat; Enhanced the presence of non-essential and flavor amino acids in muscle tissue and greatly enhanced the antioxidant capacity of broilers, leading to a noteworthy upregulation of antioxidant genes at the genetic level (<em>P</em> &lt; 0.05); Reduced in blood lipids, blood glucose, liver and abdominal fat accumulation in high-fat diet-induced obese chickens (<em>P</em> &lt; 0.05), markedly improved serum and liver biochemical parameters, and histological analysis results also demonstrated that EGCG markedly decreased hepatic lipid accumulation caused by HFD feeding. Compared to high-fat diet-induced obese chickens, supplementation of EGCG significantly lowered hepatic fatty acid synthase (FAS) expression and lipid synthesis metabolites, while fatty acid decomposition enzymes showed no significant changes. Furthermore, EGCG significantly decreased inflammation levels and oxidative damage in high-fat diet-induced obese chickens (<em>P</em> &lt; 0.05). 16S rRNA gene sequencing revealed that dietary supplementation of EGCG reduced the abundance of <em>Bacteroidota</em> and <em>Dielma</em>, while increasing the abundance of <em>Firmicutes, Turiciactor, Romboutsia</em>, and <em>Parasutterella</em>, thereby modulating the microbial composition. Dietary EGCG may have induced some of the alterations due to increased activity of the enzymes catalase (CAT) and superoxide dismutase (SOD), as well as decreased oxidation of proteins and lipids. Collectively, EGCG shows potential as an effective dietary additive for improving the high fat feeding of broiler health, feed nutrient utilization, and meat quality and nutritional value. This experiment provides a powerful new idea for the efficient utilization of oil feed and has important theoretical significance.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104875"},"PeriodicalIF":3.8,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus","authors":"Kanana Rungprasert ,&nbsp;Wikanda Tunterek ,&nbsp;Phornphisut Areeraksakul ,&nbsp;Duangduean Prakairungnamthip ,&nbsp;Chalida Sri-in ,&nbsp;Navapon Techakriengkrai ,&nbsp;Wijit Banlunara ,&nbsp;Christine A. Jansen ,&nbsp;Teerawut Nedumpun ,&nbsp;Aunyaratana Thontiravong","doi":"10.1016/j.psj.2025.104878","DOIUrl":"10.1016/j.psj.2025.104878","url":null,"abstract":"<div><div>Duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, causes severe neurological disorders and acute egg drop syndrome in ducks. Currently, several clusters of DTMUV exhibiting significant antigenic variation are circulating in Asia with distinct geographical distributions. Therefore, developing vaccines based on virus clusters specific to regions is essential. To generate an effective inactivated vaccine, the virus inactivation procedure must be optimized for each virus strain. However, no information is available on the optimal inactivation protocol for cluster 2.1 DTMUV, which predominantly circulates in several Asian countries. This study aimed to determine the effect of beta-propiolactone (BPL) inactivation on the infectivity, antigenic integrity, and immunogenicity of cluster 2.1 DTMUV. Our results demonstrated that all conditions of BPL treatment (1:2000, 1:3000, and 1:4000 (vol/vol) concentration; 24, 48, and 72 h of incubation) could completely inactivate cluster 2.1 DTMUV, as evidenced by the absence of cytopathic effect (CPE) and DTMUV antigens after 3 passages in baby hamster kidney (BHK-21) cells. However, BPL at 1:4000 (vol/vol) concentration with 24 h of incubation preserved both the total protein content and the antigenic integrity of cluster 2.1 DTMUV more effectively than other conditions. Furthermore, we found that cluster 2.1 DTMUV inactivated with BPL under this condition was safe and highly immunogenic in ducks. This was evidenced by the absence of clinical signs and the robust induction of DTMUV-specific neutralizing antibodies and T helper lymphocyte responses in immunized ducks. Overall, these findings suggest that a 1:4000 dilution of BPL with 24 h of incubation is the optimal condition for complete inactivation of cluster 2.1 DTMUV without significant loss of antigenicity and immunogenicity. This protocol can serve as a guideline for efficient cluster 2.1 DTMUV inactivation, which is valuable for vaccine and immunoassay development.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104878"},"PeriodicalIF":3.8,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}