Intestinal commensal bacteria promote Bactrocera dorsalis larval development through the vitamin B6 synthesis pathway.

IF 13.8 1区 生物学 Q1 MICROBIOLOGY Microbiome Pub Date : 2024-11-04 DOI:10.1186/s40168-024-01931-9
Jian Gu, Zhichao Yao, Bruno Lemaitre, Zhaohui Cai, Hongyu Zhang, Xiaoxue Li
{"title":"Intestinal commensal bacteria promote Bactrocera dorsalis larval development through the vitamin B6 synthesis pathway.","authors":"Jian Gu, Zhichao Yao, Bruno Lemaitre, Zhaohui Cai, Hongyu Zhang, Xiaoxue Li","doi":"10.1186/s40168-024-01931-9","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The gut microbiota can facilitate host growth under nutrient-constrained conditions. However, whether this effect is limited to certain bacterial species remains largely unclear, and the relevant underlying mechanisms remain to be thoroughly investigated.</p><p><strong>Results: </strong>We found that the microbiota was required for Bactrocera dorsalis larval growth under poor dietary conditions. Monoassociation experiments revealed that Enterobacteriaceae and some Lactobacilli promoted larval growth. Among the 27 bacterial strains tested, 14 significantly promoted larval development, and the Enterobacteriaceae cloacae isolate exhibited the most obvious promoting effect. A bacterial genome-wide association study (GWAS) revealed that the vitamin B6 synthesis pathway was critical for the promotion of E. cloacae growth. Deletion of pdxA, which is responsible for vitamin B6 biosynthesis, deprived the mutant strains of larval growth-promoting function, indicating that the 4-hydroxythreonine-4-phosphate dehydrogenase(pdxA) gene was crucial for promoting larval growth in E. cloacae. Importantly, supplementation of a poor diet with vitamin B6 successfully rescued the axenic larval growth phenotype of B. dorsalis.</p><p><strong>Conclusion: </strong>Our results suggest that gut microbes promote insect larval growth by providing vitamin B6 under nutrient scarcity conditions in B. dorsalis. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"12 1","pages":"227"},"PeriodicalIF":13.8000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533292/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiome","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s40168-024-01931-9","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: The gut microbiota can facilitate host growth under nutrient-constrained conditions. However, whether this effect is limited to certain bacterial species remains largely unclear, and the relevant underlying mechanisms remain to be thoroughly investigated.

Results: We found that the microbiota was required for Bactrocera dorsalis larval growth under poor dietary conditions. Monoassociation experiments revealed that Enterobacteriaceae and some Lactobacilli promoted larval growth. Among the 27 bacterial strains tested, 14 significantly promoted larval development, and the Enterobacteriaceae cloacae isolate exhibited the most obvious promoting effect. A bacterial genome-wide association study (GWAS) revealed that the vitamin B6 synthesis pathway was critical for the promotion of E. cloacae growth. Deletion of pdxA, which is responsible for vitamin B6 biosynthesis, deprived the mutant strains of larval growth-promoting function, indicating that the 4-hydroxythreonine-4-phosphate dehydrogenase(pdxA) gene was crucial for promoting larval growth in E. cloacae. Importantly, supplementation of a poor diet with vitamin B6 successfully rescued the axenic larval growth phenotype of B. dorsalis.

Conclusion: Our results suggest that gut microbes promote insect larval growth by providing vitamin B6 under nutrient scarcity conditions in B. dorsalis. Video Abstract.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
肠道共生细菌通过维生素 B6 合成途径促进背鳍乳杆菌幼虫的发育。
背景:肠道微生物群可在营养受限的条件下促进宿主的生长。然而,这种作用是否仅限于某些细菌种类在很大程度上仍不清楚,相关的内在机制仍有待深入研究:结果:我们发现,在恶劣的饮食条件下,背鳍乳杆菌幼虫的生长需要微生物群。单菌落实验表明,肠杆菌科细菌和一些乳酸杆菌能促进幼虫的生长。在测试的 27 株细菌中,有 14 株能显著促进幼虫发育,其中泄殖腔肠杆菌属分离株的促进作用最为明显。细菌全基因组关联研究(GWAS)发现,维生素 B6 合成途径对促进泄殖腔肠杆菌的生长至关重要。缺失了负责维生素 B6 生物合成的 pdxA 基因,突变株就失去了促进幼虫生长的功能,这表明 4-羟基苏氨酸-4-磷酸脱氢酶(pdxA)基因对促进泄殖酵母幼虫的生长至关重要。重要的是,在贫乏的饮食中补充维生素 B6 成功地挽救了 B. dorsalis 的轴突幼虫生长表型:我们的研究结果表明,在营养匮乏的条件下,肠道微生物通过提供维生素 B6 促进了背甲线虫幼虫的生长。视频摘要
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Microbiome
Microbiome MICROBIOLOGY-
CiteScore
21.90
自引率
2.60%
发文量
198
审稿时长
4 weeks
期刊介绍: Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
期刊最新文献
Correction: Parabacteroides distasonis regulates the infectivity and pathogenicity of SVCV at different water temperatures. The intestinal microbiome and Cetobacterium somerae inhibit viral infection through TLR2-type I IFN signaling axis in zebrafish. Multi-omics investigation into long-distance road transportation effects on respiratory health and immunometabolic responses in calves. The fall armyworm converts maize endophytes into its own probiotics to detoxify benzoxazinoids and promote caterpillar growth. Integrated multi-approaches reveal unique metabolic mechanisms of Vestimentifera to adapt to deep sea.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1