Bacterial isolates and antibiotic sensitivity in canine bacterial keratitis in Korea.

IF 1.7 4区农林科学 Q2 VETERINARY SCIENCES Veterinary ophthalmology Pub Date : 2024-11-03 DOI:10.1111/vop.13296

Jiwoo Park, Donghee Kim, Myeongjee Kwon, Hyun Kwon, Jungyeon Yong, Haerin Yoon, Jiyi Hwang, Ji Seung Jung, Kyung-Mee Park

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Abstract

Objective: To analyze bacterial isolates associated with canine bacterial keratitis and their antibiotic susceptibility patterns in Korea, focusing on multidrug resistance (MDR) and identifying effective antibiotic combinations for clinical treatment.

Animal studied: A total of 146 dogs diagnosed with suspected bacterial keratitis between October 2022 and October 2023 in Korea, with 157 eye samples collected for analysis.

Procedure: Eye samples were cultured to isolate bacteria, and antibiotic susceptibility testing was performed using the minimum inhibitory concentration (MIC) method. Bacterial identification was conducted using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF). The study assessed the efficacy of individual antibiotics and combination therapies.

Results: Bacteria were isolated in 55.4% of the samples. The most common genera were Staphylococcus species (48.5%, 48/99), Streptococcus species (13.1%, 13/99), Pseudomonas species (9.1%, 9/99), and Escherichia coli (9.1%, 9/99). Amikacin (84.8%) showed the highest antibiotic susceptibility, while doxycycline exhibited the lowest (17.2%). The most effective antibiotic combinations were amikacin-moxifloxacin (93%). MDR isolates accounted for 52.5% (52/99) of the total bacterial samples.

Conclusions: Staphylococcus species were the most common isolates, with 52.5% showing MDR, underscoring the need to curb antibiotic misuse. While antibiotics like amikacin demonstrated high susceptibility rates, their use should be reserved for resistant infections to prevent further resistance development. Rather than focusing solely on finding effective combinations of antibiotics, it is crucial to consider alternative treatment strategies that offer more sustainable solutions. Rather than relying on antibiotic combinations, attention should shift to sustainable alternatives to treat bacterial keratitis and reduce antibiotic dependence in clinical practice.

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韩国犬细菌性角膜炎的细菌分离和抗生素敏感性。

目的：分析韩国犬细菌性角膜炎相关细菌分离株及其抗生素敏感性模式：分析韩国犬细菌性角膜炎相关细菌分离株及其抗生素敏感性模式，重点关注多重耐药性（MDR），并确定临床治疗的有效抗生素组合：2022年10月至2023年10月期间，韩国共有146只犬被诊断为疑似细菌性角膜炎，收集了157份眼部样本进行分析：对眼部样本进行培养以分离细菌，并使用最小抑菌浓度（MIC）法进行抗生素敏感性测试。细菌鉴定采用基质辅助激光解吸/电离飞行时间法（MALDI-TOF）进行。研究评估了单种抗生素和联合疗法的疗效：结果：55.4%的样本中分离出了细菌。最常见的菌属是葡萄球菌（48.5%，48/99）、链球菌（13.1%，13/99）、假单胞菌（9.1%，9/99）和大肠埃希菌（9.1%，9/99）。阿米卡星（84.8%）的抗生素敏感性最高，而强力霉素的敏感性最低（17.2%）。最有效的抗生素组合是阿米卡星-莫西沙星（93%）。耐药菌株占细菌样本总数的 52.5%（52/99）：结论：葡萄球菌是最常见的分离菌株，其中 52.5% 为耐药菌株，这表明有必要遏制抗生素的滥用。虽然阿米卡星等抗生素的药敏率较高，但应将其用于耐药感染，以防止耐药性的进一步发展。与其只专注于寻找有效的抗生素组合，还不如考虑能提供更可持续解决方案的替代治疗策略，这一点至关重要。与其依赖抗生素组合，不如将注意力转移到治疗细菌性角膜炎的可持续替代方案上，减少临床实践中对抗生素的依赖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary ophthalmology 农林科学-兽医学

CiteScore

2.70

自引率

37.50%

发文量

审稿时长

12-24 weeks

期刊介绍： Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of: Clinical and investigational veterinary and comparative ophthalmology; Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species; Experimental models of both animal and human ocular disease in veterinary species; Anatomic studies of the animal eye; Physiological studies of the animal eye; Pharmacological studies of the animal eye.