A Novel Cortex Phellodendri Chinensis-Based Carbon Dots Platform for Remarkable Analgesia for Clinical Pain Management.

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Veterinary Medicine and Science Pub Date : 2024-11-01 DOI:10.1002/vms3.70090
Huimin Peng, Xingxing Zeng, Songbai Li, Xin Wang
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Abstract

In this study, we explored the eco-friendly synthesis of photoluminescent CCDs employing a direct one-step pyrolysis process, utilizing natural Cortex Phellodendri Chinensis as the precursor material and studied their analgesic effect in mice. The synthesized carbon dots underwent comprehensive characterization through a range of spectroscopic and microscopic techniques. These included UV-Vis, FTIR, fluorescence spectroscopy and HR-TEM, DLS instruments. HR-TEM results exhibited the presence of homogenous spherical-shaped C-dots of about 3.3 nm without aggregates. Furthermore, the prepared CCDs were studied for their in vivo analgesic effect in mice by performing tail-immersion, hot plate and acetic acid writhing tests. Also, an MTT assay was performed to assess the in vitro cytotoxicity of CCDs against L929 cells. In vitro cytotoxicity studies revealed that L929 cells exhibited higher cell viability when treated with prepared CCDs. The cellular uptake studies revealed the phase contrast images of MG-63 cells at wavelength 488 nm clearly depicted the aggregation of green, fluorescent CCDs within the cells while leaving nuclei unobscured. In addition, to the best of our understanding, the results presented in this paper showed that CCDs exhibited an important analgesic effect and enhanced anti-nociceptive activity, which may be due to stimulation of the opioidergic system. Consequently, CCDs appear to be a viable analgesic alternative for traditional analgesic candidates in pain management.

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基于黄柏的新型碳点平台为临床疼痛治疗提供显著镇痛效果
在本研究中,我们利用天然黄柏作为前驱体材料,采用一步直接热解工艺,探索了一种环保型光致发光 CCD 的合成方法,并研究了其对小鼠的镇痛效果。通过一系列光谱和显微技术对合成的碳点进行了综合表征。这些技术包括紫外可见光谱、傅立叶变换红外光谱、荧光光谱以及 HR-TEM 和 DLS 仪器。HR-TEM 结果表明,存在约 3.3 nm 的均匀球形 C 点,且无聚集体。此外,还通过尾浸、热板和醋酸蠕动试验研究了制备的 CCD 对小鼠的体内镇痛效果。此外,还进行了 MTT 试验,以评估 CCD 对 L929 细胞的体外细胞毒性。体外细胞毒性研究表明,用制备好的 CCD 处理 L929 细胞时,细胞存活率较高。细胞吸收研究显示,在波长为 488 纳米的 MG-63 细胞相衬图像中,绿色荧光 CCD 在细胞内聚集,而细胞核未被遮盖。此外,据我们所知,本文的研究结果表明,CCDs 具有重要的镇痛作用,并增强了抗痛觉活性,这可能是由于刺激了阿片肽能系统。因此,在疼痛治疗中,CCD 似乎是传统镇痛剂的一种可行替代品。
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来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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