Oxidative deterioration of polypropylene by redox mediators and yeast expressing a fungal recombinant laccase

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY International Biodeterioration & Biodegradation Pub Date : 2024-11-01 DOI:10.1016/j.ibiod.2024.105947
G. Sabellico , A. Baggetta , E. Sandrucci , G. Zanellato , A. Martinelli , A. Montanari , M.M. Bianchi
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Abstract

Biodegradation of polyolefins is a multistep process that might integrate physical, chemical, enzymatic and/or microbial actions. In this work, we report the use of the laccase redox mediators ABTS and DMP (2,2’ azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and 2,6-dimethoxyphenol, respectively), recycled by laccase activity in aerobic conditions, to induce oxidation of polypropylene film (PP): the laccase was produced in the reaction medium by a recombinant Kluyveromyces lactis yeast strain, expressing a fungal heterologous laccase gene. As a result, we evidenced the formation of oxidize groups on PP surface which increased its water wettablity. We also showed a clear modification of the polymer surface with the appearance of humped and exfoliated regions which favored the break of PP film.

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氧化还原介质和表达真菌重组漆酶的酵母对聚丙烯的氧化劣化作用
聚烯烃的生物降解是一个综合了物理、化学、酶和/或微生物作用的多步骤过程。在这项工作中,我们报告了使用漆酶氧化还原介质 ABTS 和 DMP(分别为 2,2'叠氮双(3-乙基苯并噻唑啉-6-磺酸)和 2,6-二甲氧基苯酚)(在有氧条件下通过漆酶活性回收)来诱导聚丙烯薄膜(PP)氧化的情况:漆酶是由表达真菌异源漆酶基因的重组乳酸克鲁维酵母菌株在反应介质中产生的。结果表明,聚丙烯表面氧化基团的形成增加了其水润性。我们还发现聚合物表面发生了明显的改性,出现了驼峰和剥落区域,这有利于 PP 薄膜的断裂。
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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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