International Biodeterioration & Biodegradation最新文献

Identification of the key factors influencing biodeterioration of open-air cultural heritage in the temperate climate zone of China 确定影响中国温带气候区露天文物生物退化的关键因素

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-16 DOI: 10.1016/j.ibiod.2024.105954

Chong Wang , Li Wang , Chongbin Bai , Mingpeng Wang , Tao Ma , Honglin Ma , Guangming Zhang , Weiwei Wang , Zisheng Guo , Yanmei Sun , Shiwei Wang

Biodeterioration is known to cause irreversible damage to open-air cultural heritages. China has numerous open-air cultural heritages constructed of stone and brick, but very little information is available about the factors involved in their biodeterioration. In this study, different kinds of open-air cultural heritages (steles, towers, temples, Tang-ling carvings, and stone bridges) from 14 different locations in temperate climate zone of China were collected and investigated. Microbiological characteristics of these cultural heritages revealed that both cyanobacteria and lichens were the mainly dominant groups on these open-air stone and brick cultural heritages with visible deterioration. Cyanobacteria were closely associated with the severely deteriorated Tang-ling carvings wile lichens were most commonly found on temples and bridges. The main cyanobacteria were Oscillatoria, Pleurocapsa, and Lyngbya, and the dominant lichens were Xanthoria Th. Fr., Lepraria. Ach. and Camellia on the surface of cultural heritage. In addition, the extent of deterioration associated with cyanobacteria exhibited an increasing trend with higher annual mean sunshine intensity, calcite content, and cultural heritage age though not statistically significant. Redundancy analysis indicated that sunshine intensity and the age of the archeological structures were the key factors that significantly affected the biodeterioration of cultural heritages. This study provides more understanding of the microbial community associated with deterioration of open-air cultural heritages situated at the temperate climate zone of China.

众所周知，生物退化会对露天文物造成不可逆转的破坏。中国有大量的石质和砖质露天文物，但有关其生物劣化因素的信息却很少。本研究收集并调查了中国温带气候区 14 个不同地点的各种露天文物（石碑、塔、寺庙、唐陵雕刻和石桥）。这些文物的微生物学特征表明，蓝藻和地衣是这些有明显退化的露天石砖文物上的主要优势菌群。蓝藻与严重破损的唐陵石刻密切相关，而地衣则多见于寺庙和桥梁。主要的蓝藻有 Oscillatoria、Pleurocapsa 和 Lyngbya，主要的地衣有 Xanthoria Th. Fr.Fr.、Lepraria.Ach. 和山茶花。此外，随着年平均日照强度、方解石含量和文物年代的增加，与蓝藻相关的劣化程度呈上升趋势，但无统计学意义。冗余分析表明，日照强度和考古结构的年代是显著影响文物生物劣化的关键因素。本研究有助于进一步了解中国温带气候区露天文物退化的相关微生物群落。

{"title":"Identification of the key factors influencing biodeterioration of open-air cultural heritage in the temperate climate zone of China","authors":"Chong Wang , Li Wang , Chongbin Bai , Mingpeng Wang , Tao Ma , Honglin Ma , Guangming Zhang , Weiwei Wang , Zisheng Guo , Yanmei Sun , Shiwei Wang","doi":"10.1016/j.ibiod.2024.105954","DOIUrl":"10.1016/j.ibiod.2024.105954","url":null,"abstract":"<div><div>Biodeterioration is known to cause irreversible damage to open-air cultural heritages. China has numerous open-air cultural heritages constructed of stone and brick, but very little information is available about the factors involved in their biodeterioration. In this study, different kinds of open-air cultural heritages (steles, towers, temples, Tang-ling carvings, and stone bridges) from 14 different locations in temperate climate zone of China were collected and investigated. Microbiological characteristics of these cultural heritages revealed that both cyanobacteria and lichens were the mainly dominant groups on these open-air stone and brick cultural heritages with visible deterioration. Cyanobacteria were closely associated with the severely deteriorated Tang-ling carvings wile lichens were most commonly found on temples and bridges. The main cyanobacteria were <em>Oscillatoria</em>, <em>Pleurocapsa</em>, and <em>Lyngbya</em>, and the dominant lichens were <em>Xanthoria</em> Th. Fr., <em>Lepraria</em>. Ach. and <em>Camellia</em> on the surface of cultural heritage. In addition, the extent of deterioration associated with cyanobacteria exhibited an increasing trend with higher annual mean sunshine intensity, calcite content, and cultural heritage age though not statistically significant. Redundancy analysis indicated that sunshine intensity and the age of the archeological structures were the key factors that significantly affected the biodeterioration of cultural heritages. This study provides more understanding of the microbial community associated with deterioration of open-air cultural heritages situated at the temperate climate zone of China.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105954"},"PeriodicalIF":4.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impacts of flagellin on larval metamorphosis of mussel Mytilus coruscus varied with protein structure 鞭毛蛋白对贻贝幼体变态的影响因蛋白质结构而异

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-15 DOI: 10.1016/j.ibiod.2024.105952

Tiantian Liu , Yu Tao , Yushan Cai , Lihua Peng , Jin-Long Yang , Xiao Liang

Flagellin produced by Pseudoalteromonas marina has been shown to induce larval metamorphosis in mussels. The flagellin protein FliC was composed of four subunits encoded by the genes fliC-02330, fliC-02345, fliC-02346, and fliC-02347. In this study, we investigated the effects of these four FliC proteins on biofilm formation and larval metamorphosis. The inducing activity of the recombinant proteins FliC-02345, FliC-02346, and FliC-02347 was similar and significantly higher (p < 0.05) than that of FliC-02330. When compared to the wild-type strain, biofilm formation was significantly enhanced in the four fliC mutants, whereas the inducing activity of four ΔfliC biofilms decreased. Among these mutants, ΔfliC-02330 exhibited the highest thickness, density, and c-di-GMP levels among its respective biofilms. The inducing activity of the ΔfliC-02330 biofilm increased by 30–40% compared to the inducing activities of other mutant strains. Furthermore, structural analysis revealed differences in protein structure and phosphorylation sites between FliC-02330 and its counterparts (FliC-02345, FliC-02346, and FliC-02347), suggesting that variations in protein structure contribute to differing impacts on both biofilm formation and larval metamorphosis. These findings provide new insights into the interaction between bacterial flagellin protein and the larval metamorphosis of marine invertebrates.

已证明假交替单胞菌（Pseudoalteromonas marina）产生的鞭毛蛋白可诱导贻贝幼虫变态。鞭毛蛋白 FliC 由四个亚基组成，分别由 fliC-02330、fliC-02345、fliC-02346 和 fliC-02347 基因编码。在本研究中，我们研究了这四种 FliC 蛋白对生物膜形成和幼虫变态的影响。重组蛋白FliC-02345、FliC-02346和FliC-02347的诱导活性与FliC-02330相似，且显著高于FliC-02330（p <0.05）。与野生型菌株相比，四个 fliC 突变体的生物膜形成明显增强，而四个 ΔfliC 生物膜的诱导活性降低。在这些突变体中，ΔfliC-02330 的生物膜厚度、密度和 c-di-GMP 水平最高。与其他突变株的诱导活性相比，ΔfliC-02330 生物膜的诱导活性提高了 30-40%。此外，结构分析表明，FliC-02330 与其对应品系（FliC-02345、FliC-02346 和 FliC-02347）的蛋白质结构和磷酸化位点存在差异，这表明蛋白质结构的变化对生物膜的形成和幼虫的变态产生了不同的影响。这些发现为了解细菌鞭毛蛋白与海洋无脊椎动物幼虫变态之间的相互作用提供了新的视角。

{"title":"The impacts of flagellin on larval metamorphosis of mussel Mytilus coruscus varied with protein structure","authors":"Tiantian Liu , Yu Tao , Yushan Cai , Lihua Peng , Jin-Long Yang , Xiao Liang","doi":"10.1016/j.ibiod.2024.105952","DOIUrl":"10.1016/j.ibiod.2024.105952","url":null,"abstract":"<div><div>Flagellin produced by <em>Pseudoalteromonas marina</em> has been shown to induce larval metamorphosis in mussels. The flagellin protein FliC was composed of four subunits encoded by the genes <em>fliC-02330</em>, <em>fliC-02345</em>, <em>fliC-02346</em>, and <em>fliC-02347</em>. In this study, we investigated the effects of these four FliC proteins on biofilm formation and larval metamorphosis. The inducing activity of the recombinant proteins FliC-02345, FliC-02346, and FliC-02347 was similar and significantly higher (<em>p</em> < 0.05) than that of FliC-02330. When compared to the wild-type strain, biofilm formation was significantly enhanced in the four <em>fliC</em> mutants, whereas the inducing activity of four Δ<em>fliC</em> biofilms decreased. Among these mutants, Δ<em>fliC</em>-02330 exhibited the highest thickness, density, and c-di-GMP levels among its respective biofilms. The inducing activity of the Δ<em>fliC-02330</em> biofilm increased by 30–40% compared to the inducing activities of other mutant strains. Furthermore, structural analysis revealed differences in protein structure and phosphorylation sites between FliC-02330 and its counterparts (FliC-02345, FliC-02346, and FliC-02347), suggesting that variations in protein structure contribute to differing impacts on both biofilm formation and larval metamorphosis. These findings provide new insights into the interaction between bacterial flagellin protein and the larval metamorphosis of marine invertebrates.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105952"},"PeriodicalIF":4.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehending microplastic pollution in diverse environment: Assessing fate, impacts, and remediation approaches 了解不同环境中的微塑料污染：评估归宿、影响和补救方法

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-12 DOI: 10.1016/j.ibiod.2024.105953

Priyanka Yadav , Vishal Mishra

Microplastics (MPs) pose pervasive environmental threats that potentially harm ecosystems and public health. Till date, most of the researches have predominantly concentrated on freshwater and marine ecosystems. This review attempts to provide current knowledge of microplastic pollution, its source, and its fate in all environmental compartments for instance soil, water, and atmosphere. Also briefly summarizes different MP's exposure routes and their negative consequences on public health. Various conventional methods have been used to remove MPs but these are inefficient owing to several demerits. Microbial approach is regarded as a safer option for removing MPs due to its unique metabolic mechanism. More than 400 microorganisms have been discovered as capable of MPs degradation. Fungi have appeared as the most proficient degraders of plastics among the microbes because they release unique enzymes and can thrive on hazardous components with low resources. Numerous fungal species that can break down various plastic kinds have been found in recent studies; nevertheless, there are still many unanswered questions regarding the mechanisms underlying biodegradation. The molecular mechanisms behind MP degradation via various enzymes have received little attention. Also, white-rot fungi have received the least attention among the fungi examined for biodegradation despite having a unique extracellular enzymatic system. Thus, the current study summarizes the ability of fungi that degrade various polymers and the function of multiple enzymes in the degradation of MPs. Also, the role of different extracellular and intracellular enzymes in biological degradation of MPs is discussed. Prospects for the future include tracking long-term trends in microplastic contamination, evaluating the effectiveness of clean-up techniques, and integrating the developed technologies with socioeconomic factors into policy frameworks.

微塑料（MPs）对环境构成了普遍威胁，有可能损害生态系统和公众健康。迄今为止，大多数研究主要集中在淡水和海洋生态系统。本综述试图提供有关微塑料污染、其来源及其在土壤、水和大气等所有环境区划中的归宿的最新知识。此外，还简要概述了不同微塑料的接触途径及其对公众健康的负面影响。人们使用了各种传统方法来清除 MPs，但这些方法由于存在一些缺点而效率低下。微生物方法因其独特的新陈代谢机制而被认为是去除 MPs 的更安全选择。目前已发现 400 多种能够降解 MPs 的微生物。真菌是微生物中最擅长降解塑料的，因为它们能释放独特的酶，并能以较少的资源在有害成分上生长。最近的研究发现了许多能分解各种塑料的真菌种类；然而，关于生物降解的基本机制仍有许多未解之谜。通过各种酶降解 MP 的分子机制很少受到关注。此外，尽管白腐真菌具有独特的胞外酶系统，但在所研究的生物降解真菌中，白腐真菌受到的关注最少。因此，本研究总结了真菌降解各种聚合物的能力以及多种酶在降解 MPs 过程中的作用。此外，还讨论了不同的细胞外酶和细胞内酶在生物降解 MPs 中的作用。未来展望包括跟踪微塑料污染的长期趋势、评估清理技术的有效性，以及将开发的技术与社会经济因素纳入政策框架。

{"title":"Comprehending microplastic pollution in diverse environment: Assessing fate, impacts, and remediation approaches","authors":"Priyanka Yadav , Vishal Mishra","doi":"10.1016/j.ibiod.2024.105953","DOIUrl":"10.1016/j.ibiod.2024.105953","url":null,"abstract":"<div><div>Microplastics (MPs) pose pervasive environmental threats that potentially harm ecosystems and public health. Till date, most of the researches have predominantly concentrated on freshwater and marine ecosystems. This review attempts to provide current knowledge of microplastic pollution, its source, and its fate in all environmental compartments for instance soil, water, and atmosphere. Also briefly summarizes different MP's exposure routes and their negative consequences on public health. Various conventional methods have been used to remove MPs but these are inefficient owing to several demerits. Microbial approach is regarded as a safer option for removing MPs due to its unique metabolic mechanism. More than 400 microorganisms have been discovered as capable of MPs degradation. Fungi have appeared as the most proficient degraders of plastics among the microbes because they release unique enzymes and can thrive on hazardous components with low resources. Numerous fungal species that can break down various plastic kinds have been found in recent studies; nevertheless, there are still many unanswered questions regarding the mechanisms underlying biodegradation. The molecular mechanisms behind MP degradation via various enzymes have received little attention. Also, white-rot fungi have received the least attention among the fungi examined for biodegradation despite having a unique extracellular enzymatic system. Thus, the current study summarizes the ability of fungi that degrade various polymers and the function of multiple enzymes in the degradation of MPs. Also, the role of different extracellular and intracellular enzymes in biological degradation of MPs is discussed. Prospects for the future include tracking long-term trends in microplastic contamination, evaluating the effectiveness of clean-up techniques, and integrating the developed technologies with socioeconomic factors into policy frameworks.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105953"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative studies on the effectiveness of proteases and enzymes from dietary supplements in the degradation of polylactide (PLA) 蛋白酶和膳食补充剂中的酶在降解聚乳酸（PLA）中的效果比较研究

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-10 DOI: 10.1016/j.ibiod.2024.105950

Katarzyna Janczak , Alicja Mazuryk , Daria Lisewska , Natalia Puszczykowska , Kacper Fiedurek

Polylactide (PLA) has become one of the most widely used biodegradable plastics in recent years. The research focuses on solving the problem of its low-rate biodegradability under mesophilic conditions using cost-effective proteases of alternative origin. The aim of the study was to verify whether enzymes contained in food dietary supplements with the suggested high activity of protease enzymes could potentially be used for the degradation of PLA. The studies compare the activity of enzymes from dietary supplements with hydrolytic enzymes with scientifically proven effectiveness in degrading PLA. Products containing nattokinase, bromelain, papain and an enzyme mixture were tested. Their effectiveness against proteinase K, subtilisin and lipase was compared. From this group, proteinase K caused the greatest PLA mass loss and increase in O/C ratio after 28 days of incubation. The results indicated that nattokinase and papain caused a mass loss of PLA films similar to that of proteinase K, as well as a decrease in the carbonyl index value by more than 20%. Additionally, studies have shown that dietary supplements have a beneficial effect on the compost microflora. Causing an approximately 20-fold increase in the number of microorganisms within 48 h. In accordance with the assumptions, enzymatic products and their concentrations were selected that effectively accelerate PLA degradation in mesophilic conditions at a level comparable to or better than proteinase K, but have a much better effect on the substrate microflora, which makes them an interesting object for the development of products for accelerating the degradation of polymeric materials. In order to confirm the possibility of practical use of the results, an analysis of the phytotoxicity of the enzymes was carried out, which confirmed the possibility of using the enzymes in natural conditions.

聚乳酸（PLA）是近年来应用最广泛的生物降解塑料之一。这项研究的重点是利用具有成本效益的替代来源蛋白酶，解决聚乳酸在中温条件下生物降解率低的问题。研究的目的是验证食品膳食补充剂中所含的酶是否具有蛋白酶的高活性，是否有可能用于降解聚乳酸。研究将膳食补充剂中的酶的活性与经科学证明可有效降解聚乳酸的水解酶进行了比较。对含有纳豆激酶、菠萝蛋白酶、木瓜蛋白酶和酶混合物的产品进行了测试。比较了它们对蛋白酶 K、枯草酶和脂肪酶的功效。在这组产品中，蛋白酶 K 在培养 28 天后造成的聚乳酸质量损失和 O/C 比率增加最大。结果表明，纳豆激酶和木瓜蛋白酶造成的聚乳酸薄膜质量损失与蛋白酶 K 相似，羰基指数值下降了 20% 以上。此外，研究还表明，膳食补充剂对堆肥微生物区系有好处。根据上述假设，我们选择了酶制剂产品及其浓度，它们能有效加速聚乳酸在中温条件下的降解，其降解效果与蛋白酶 K 相当或更好，但对底物微生物群的影响要大得多，这使它们成为开发加速高分子材料降解产品的有趣对象。为了证实这些结果的实际应用可能性，对酶的植物毒性进行了分析，证实了在自然条件下使用酶的可能性。

{"title":"Comparative studies on the effectiveness of proteases and enzymes from dietary supplements in the degradation of polylactide (PLA)","authors":"Katarzyna Janczak , Alicja Mazuryk , Daria Lisewska , Natalia Puszczykowska , Kacper Fiedurek","doi":"10.1016/j.ibiod.2024.105950","DOIUrl":"10.1016/j.ibiod.2024.105950","url":null,"abstract":"<div><div>Polylactide (PLA) has become one of the most widely used biodegradable plastics in recent years. The research focuses on solving the problem of its low-rate biodegradability under mesophilic conditions using cost-effective proteases of alternative origin. The aim of the study was to verify whether enzymes contained in food dietary supplements with the suggested high activity of protease enzymes could potentially be used for the degradation of PLA. The studies compare the activity of enzymes from dietary supplements with hydrolytic enzymes with scientifically proven effectiveness in degrading PLA. Products containing nattokinase, bromelain, papain and an enzyme mixture were tested. Their effectiveness against proteinase K, subtilisin and lipase was compared. From this group, proteinase K caused the greatest PLA mass loss and increase in O/C ratio after 28 days of incubation. The results indicated that nattokinase and papain caused a mass loss of PLA films similar to that of proteinase K, as well as a decrease in the carbonyl index value by more than 20%. Additionally, studies have shown that dietary supplements have a beneficial effect on the compost microflora. Causing an approximately 20-fold increase in the number of microorganisms within 48 h. In accordance with the assumptions, enzymatic products and their concentrations were selected that effectively accelerate PLA degradation in mesophilic conditions at a level comparable to or better than proteinase K, but have a much better effect on the substrate microflora, which makes them an interesting object for the development of products for accelerating the degradation of polymeric materials. In order to confirm the possibility of practical use of the results, an analysis of the phytotoxicity of the enzymes was carried out, which confirmed the possibility of using the enzymes in natural conditions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105950"},"PeriodicalIF":4.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation approaches for chromium detoxification and transformation: Advanced strategies and future Perspectives 铬解毒和转化的生物修复方法：先进战略与未来展望

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-09 DOI: 10.1016/j.ibiod.2024.105951

Arukula Deepa , Anthati Mastan , Viswanath Buddolla , Yedluri Anil Kumar , Buddolla Anantha Lakshmi , Young-Joon Kim

Hexavalent chromium [Cr(VI)] contamination poses a significant environmental and public health risk due to its widespread industrial use, toxic nature, and carcinogenic properties. Conventional Cr(VI) remediation methods, including electrochemical processes, ion exchange, reverse osmosis, and adsorption, face challenges such as high energy consumption and incomplete detoxification. This review provides a comprehensive evaluation of cutting-edge bioremediation techniques for Cr(VI) removal, with an emphasis on microbial mechanisms involving bacteria and fungi. Bioremediation approaches, particularly those utilizing Cr(VI)-resistant microorganisms, present promising, eco-friendly, and cost-effective solutions for chromium pollution mitigation. The mechanisms of biosorption, bioaccumulation, and bio-reduction employed by these microorganisms are thoroughly examined. Furthermore, the review addresses the scalability and practical application of these biotechnologies in wastewater treatment facilities. This analysis highlights the critical need for innovative, sustainable chromium bioremediation strategies and outlines future research directions to advance this vital field.

六价铬[Cr(VI)]污染因其广泛的工业用途、毒性和致癌特性，对环境和公共健康构成了重大风险。包括电化学过程、离子交换、反渗透和吸附在内的传统六价铬修复方法面临着高能耗和解毒不彻底等挑战。本综述全面评估了去除六价铬的尖端生物修复技术，重点是涉及细菌和真菌的微生物机制。生物修复方法，特别是那些利用抗六价铬微生物的方法，为减轻铬污染提供了前景广阔、生态友好和经济高效的解决方案。综述深入研究了这些微生物的生物吸附、生物累积和生物还原机制。此外，研究还探讨了这些生物技术在废水处理设施中的可扩展性和实际应用。这项分析强调了对创新型、可持续铬生物修复战略的迫切需要，并概述了推进这一重要领域的未来研究方向。

{"title":"Bioremediation approaches for chromium detoxification and transformation: Advanced strategies and future Perspectives","authors":"Arukula Deepa , Anthati Mastan , Viswanath Buddolla , Yedluri Anil Kumar , Buddolla Anantha Lakshmi , Young-Joon Kim","doi":"10.1016/j.ibiod.2024.105951","DOIUrl":"10.1016/j.ibiod.2024.105951","url":null,"abstract":"<div><div>Hexavalent chromium [Cr(VI)] contamination poses a significant environmental and public health risk due to its widespread industrial use, toxic nature, and carcinogenic properties. Conventional Cr(VI) remediation methods, including electrochemical processes, ion exchange, reverse osmosis, and adsorption, face challenges such as high energy consumption and incomplete detoxification. This review provides a comprehensive evaluation of cutting-edge bioremediation techniques for Cr(VI) removal, with an emphasis on microbial mechanisms involving bacteria and fungi. Bioremediation approaches, particularly those utilizing Cr(VI)-resistant microorganisms, present promising, eco-friendly, and cost-effective solutions for chromium pollution mitigation. The mechanisms of biosorption, bioaccumulation, and bio-reduction employed by these microorganisms are thoroughly examined. Furthermore, the review addresses the scalability and practical application of these biotechnologies in wastewater treatment facilities. This analysis highlights the critical need for innovative, sustainable chromium bioremediation strategies and outlines future research directions to advance this vital field.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105951"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-fermentation of dark septate endophytes and Trichoderma viride can effectively degrade cellulose in different Chinese medicinal herbal residues 暗色隔内生菌和毛霉共同发酵可有效降解不同中药渣中的纤维素

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-05 DOI: 10.1016/j.ibiod.2024.105949

Wanyun Li , Menghui Wang , Xuemin Zhang , Yali Xie , Wenjing Chen , Li Yao , Chao He , Xueli He

Chinese medicinal herbal residues (CMHRs) are currently a widely overlooked renewable biomass resource, and developing controllable microbial fermentation technology for CMHRs will help achieve the resource utilization of CMHRs. In this study, the activity of various cellulases, reducing sugar content and cellulose degradation rate were combined to evaluate the solid state fermentation (SSF) potential of dark septate endophytes (DSE) and Trichoderma viride in separate fermentation and co-fermentation of different CMHRs (single Astragalus mongolicus residue and compound residues of Panax notoginseng and Salvia miltiorrhiza). The results showed that the cellulase activity of some DSE strains was higher than that of T. viride. T. viride fermentation alone could increase the content of substrate reducing sugars, while DSE fermentation could reduce the content of substrate reducing sugars. DSE strains such as Macrophomina pseudophaseoline, Paraboeremia selaginellae, Paraphoma chlamydocopiosa and Paraphoma radicina had the potential to co-ferment with T. viride. Optimization of the fermentation process by inoculation of T. viride after 3 d or 6 d of DSE effectively increased the cellulase activity of co-fermented CMHRs. After optimization, the activities of filter paper cellulase, carboxymethyl cellulase, and β-glucosidase in different fermentation combinations reached 2.51–5.61, 2.86–4.72 and 12.85–32.35 U/g, respectively. Further, the co-fermentation of DSE and T. viride effectively degraded cellulose in different CMHRs with a degradation rate of 54.43%–67.38%. Scanning electron microscope confirmed that the co-fermentation of DSE and T. viride could increase the external surface area and porosity of CMHRs, and destroy the structure of CMHRs. The results of this study both provided a basis for the microbial degradation of single and compound CMHR, which is of great significance for solving the treatment of waste such as Chinese herbal residue and further resource utilization.

中药渣是目前被广泛忽视的可再生生物质资源，开发可控的中药渣微生物发酵技术将有助于实现中药渣的资源化利用。本研究结合各种纤维素酶的活性、还原糖含量和纤维素降解率，评估了暗隔内生菌（DSE）和毛霉菌在不同中草药残体（单一黄芪残体、三七和丹参的复合残体）的单独发酵和联合发酵中的固态发酵（SSF）潜力。结果表明，一些 DSE 菌株的纤维素酶活性高于毒死蜱。单纯的病毒发酵可增加底物还原糖的含量，而 DSE 发酵则可降低底物还原糖的含量。DSE菌株，如假嗜酸性大酵母菌、Paraboeremia selaginellae、Paraphoma chlamydocopiosa和Paraphoma radicina，具有与T. viride共同发酵的潜力。通过在 DSE 3 d 或 6 d 后接种 T. viride 来优化发酵过程，可有效提高共发酵 CMHR 的纤维素酶活性。优化后，不同发酵组合的滤纸纤维素酶、羧甲基纤维素酶和β-葡萄糖苷酶的活性分别达到了2.51-5.61、2.86-4.72和12.85-32.35 U/g 。此外，DSE 和毒死蜱共同发酵可有效降解不同 CMHR 中的纤维素，降解率为 54.43%-67.38%。扫描电子显微镜证实，DSE 和病毒菌共同发酵能增加 CMHR 的外表面积和孔隙率，破坏 CMHR 的结构。该研究结果为微生物降解单一和复合中草药残渣提供了依据，对解决中草药残渣等废弃物的处理和进一步资源化利用具有重要意义。

{"title":"Co-fermentation of dark septate endophytes and Trichoderma viride can effectively degrade cellulose in different Chinese medicinal herbal residues","authors":"Wanyun Li , Menghui Wang , Xuemin Zhang , Yali Xie , Wenjing Chen , Li Yao , Chao He , Xueli He","doi":"10.1016/j.ibiod.2024.105949","DOIUrl":"10.1016/j.ibiod.2024.105949","url":null,"abstract":"<div><div>Chinese medicinal herbal residues (CMHRs) are currently a widely overlooked renewable biomass resource, and developing controllable microbial fermentation technology for CMHRs will help achieve the resource utilization of CMHRs. In this study, the activity of various cellulases, reducing sugar content and cellulose degradation rate were combined to evaluate the solid state fermentation (SSF) potential of dark septate endophytes (DSE) and <em>Trichoderma viride</em> in separate fermentation and co-fermentation of different CMHRs (single <em>Astragalus mongolicus</em> residue and compound residues of <em>Panax notoginseng</em> and <em>Salvia miltiorrhiza</em>). The results showed that the cellulase activity of some DSE strains was higher than that of <em>T. viride</em>. <em>T. viride</em> fermentation alone could increase the content of substrate reducing sugars, while DSE fermentation could reduce the content of substrate reducing sugars. DSE strains such as <em>Macrophomina pseudophaseoline</em>, <em>Paraboeremia selaginellae</em>, <em>Paraphoma chlamydocopiosa</em> and <em>Paraphoma radicina</em> had the potential to co-ferment with <em>T. viride</em>. Optimization of the fermentation process by inoculation of <em>T</em>. <em>viride</em> after 3 d or 6 d of DSE effectively increased the cellulase activity of co-fermented CMHRs. After optimization, the activities of filter paper cellulase, carboxymethyl cellulase, and β-glucosidase in different fermentation combinations reached 2.51–5.61, 2.86–4.72 and 12.85–32.35 U/g, respectively. Further, the co-fermentation of DSE and <em>T. viride</em> effectively degraded cellulose in different CMHRs with a degradation rate of 54.43%–67.38%. Scanning electron microscope confirmed that the co-fermentation of DSE and <em>T. viride</em> could increase the external surface area and porosity of CMHRs, and destroy the structure of CMHRs. The results of this study both provided a basis for the microbial degradation of single and compound CMHR, which is of great significance for solving the treatment of waste such as Chinese herbal residue and further resource utilization.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105949"},"PeriodicalIF":4.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basalt rock weathering by Peribacillus simplex from Wudalianchi volcanos in NE China and implications for Fe and Si biogeochemical cycling 中国东北五大连池火山的玄武岩岩石风化及对铁和硅生物地球化学循环的影响

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-02 DOI: 10.1016/j.ibiod.2024.105948

Shuang Zhang , Gaofei Ying , Tao Liu , Jiani Yang , Enci Zhu , Xindi Sun , Ji-Dong Gu , Lei Yan

Bacteria have an important role in weathering the different rocks, but little is known about the mechanism of microbial weathering in basalt rocks. In the present study, Peribacillus simplex WS-L19 isolated from Wudalianchi volcanos exhibits the highest Fe and Si rock dissolution, which revealed unique weathering ability. The optimal weathering conditions of LB medium diluted at 30.9%, the rotation speed of 127.2 rpm, and temperature at 36.6 °C, resulted in the Fe and Si releases of 1.70 ± 0.09 μg/mL and 20.11 ± 1.13 μg/mL, respectively. Simultaneously, complex ligands such as organic acids (tartaric acid, formic acid, lactic acid and succinic acid), siderophores, and biofilm were also found to be involved in weathering processes by P. simplex WS-L19. Kinetics study of Fe and Si release indicated that the weathering processes of volcanic rocks mediated by P. simplex WS-19 fits a Hyperbl model. Mineralogical analysis showed a decrease in Fe releasing ratio by 44.65% and an increase in Si releasing ratio by 28.45%, showing that Fe is more accessible than Si. P. simplex WS-L19 had a significant weathering effect on biotite, and Si-O bonding vibration might contribute to weathering. The results suggest that indigenous P. simplex WS-L19 plays a role in weathering Wudalianchi volcanos and provides new insights into the Fe and Si biogeochemical cycling of basalt rocks.

细菌在不同岩石的风化过程中发挥着重要作用，但人们对玄武岩微生物风化的机理知之甚少。在本研究中，从五大连池火山中分离出的单纯弧菌 WS-L19 对岩石中铁和硅的溶解度最高，显示了其独特的风化能力。在稀释度为 30.9% 的 LB 培养基、转速为 127.2 rpm、温度为 36.6 °C 的最佳风化条件下，Fe 和 Si 的释放量分别为 1.70 ± 0.09 μg/mL 和 20.11 ± 1.13 μg/mL。与此同时，还发现有机酸（酒石酸、甲酸、乳酸和琥珀酸）、嗜硒体和生物膜等复合配体也参与了单纯栉水母 WS-L19 的风化过程。铁和硅释放动力学研究表明，单胞菌 WS-19 介导的火山岩风化过程符合 Hyperbl 模型。矿物学分析表明，铁的释放率降低了 44.65%，而硅的释放率提高了 28.45%，这表明铁比硅更容易获得。单胞菌 WS-L19 对黑云母有明显的风化作用，Si-O 键振动可能有助于风化。研究结果表明，本地单胞菌 WS-L19 在五大连池火山风化过程中发挥了作用，并为玄武岩中铁和硅的生物地球化学循环提供了新的见解。

{"title":"Basalt rock weathering by Peribacillus simplex from Wudalianchi volcanos in NE China and implications for Fe and Si biogeochemical cycling","authors":"Shuang Zhang , Gaofei Ying , Tao Liu , Jiani Yang , Enci Zhu , Xindi Sun , Ji-Dong Gu , Lei Yan","doi":"10.1016/j.ibiod.2024.105948","DOIUrl":"10.1016/j.ibiod.2024.105948","url":null,"abstract":"<div><div>Bacteria have an important role in weathering the different rocks, but little is known about the mechanism of microbial weathering in basalt rocks. In the present study, <em>Peribacillus simplex</em> WS-L19 isolated from Wudalianchi volcanos exhibits the highest Fe and Si rock dissolution, which revealed unique weathering ability. The optimal weathering conditions of LB medium diluted at 30.9%, the rotation speed of 127.2 rpm, and temperature at 36.6 °C, resulted in the Fe and Si releases of 1.70 ± 0.09 μg/mL and 20.11 ± 1.13 μg/mL, respectively. Simultaneously, complex ligands such as organic acids (tartaric acid, formic acid, lactic acid and succinic acid), siderophores, and biofilm were also found to be involved in weathering processes by <em>P. simplex</em> WS-L19. Kinetics study of Fe and Si release indicated that the weathering processes of volcanic rocks mediated by <em>P. simplex</em> WS-19 fits a Hyperbl model. Mineralogical analysis showed a decrease in Fe releasing ratio by 44.65% and an increase in Si releasing ratio by 28.45%, showing that Fe is more accessible than Si. <em>P. simplex</em> WS-L19 had a significant weathering effect on biotite, and Si-O bonding vibration might contribute to weathering. The results suggest that indigenous <em>P. simplex</em> WS-L19 plays a role in weathering Wudalianchi volcanos and provides new insights into the Fe and Si biogeochemical cycling of basalt rocks.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105948"},"PeriodicalIF":4.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative deterioration of polypropylene by redox mediators and yeast expressing a fungal recombinant laccase 氧化还原介质和表达真菌重组漆酶的酵母对聚丙烯的氧化劣化作用

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-01 DOI: 10.1016/j.ibiod.2024.105947

G. Sabellico , A. Baggetta , E. Sandrucci , G. Zanellato , A. Martinelli , A. Montanari , M.M. Bianchi

Biodegradation of polyolefins is a multistep process that might integrate physical, chemical, enzymatic and/or microbial actions. In this work, we report the use of the laccase redox mediators ABTS and DMP (2,2’ azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and 2,6-dimethoxyphenol, respectively), recycled by laccase activity in aerobic conditions, to induce oxidation of polypropylene film (PP): the laccase was produced in the reaction medium by a recombinant Kluyveromyces lactis yeast strain, expressing a fungal heterologous laccase gene. As a result, we evidenced the formation of oxidize groups on PP surface which increased its water wettablity. We also showed a clear modification of the polymer surface with the appearance of humped and exfoliated regions which favored the break of PP film.

聚烯烃的生物降解是一个综合了物理、化学、酶和/或微生物作用的多步骤过程。在这项工作中，我们报告了使用漆酶氧化还原介质 ABTS 和 DMP（分别为 2,2'叠氮双（3-乙基苯并噻唑啉-6-磺酸）和 2,6-二甲氧基苯酚）（在有氧条件下通过漆酶活性回收）来诱导聚丙烯薄膜（PP）氧化的情况：漆酶是由表达真菌异源漆酶基因的重组乳酸克鲁维酵母菌株在反应介质中产生的。结果表明，聚丙烯表面氧化基团的形成增加了其水润性。我们还发现聚合物表面发生了明显的改性，出现了驼峰和剥落区域，这有利于 PP 薄膜的断裂。

引用次数: 0

ABTS mediator enhances biotransformation of fluoranthene by Laccases from Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12 ABTS 媒介物增强了嗜酸毛霉 FLU1 和嗜酸塔拉酵母菌 FLU12 的 Laccases 对荧蒽的生物转化作用

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-10-30 DOI: 10.1016/j.ibiod.2024.105946

Samson O. Egbewale , Ajit Kumar , Tosin A. Olasehinde , Mduduzi P. Mokoena , Ademola O. Olaniran

Fluoranthene poses a significant environmental threat due to its persistence and toxicity. The Laccases from Trichoderma lixii FLU1 (TlFLU1L) and Talaromyces pinophilus FLU12 (TpFLU12L) are shown to act as biocatalysts for fluoranthene degradation. 3 U/mL of TlFLU1L and TpFLU12L reduced residual fluoranthene concentration to 49.5 ± 8.68 and 61.0 ± 5.66 %, while 10U/mL to 19.2 ± 5.95 and 28.7 ± 1.25 %, respectively, in 96 h. Mixing 200 μM ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with 3 U/mL enzyme in the reaction abolished 100 % residual fluoranthene within 48 h. TlFLU1L and TpFLU12L exhibited almost similar v_max (1.35 ± 0.02 and 1.29 ± 0.15 mg/L/h, respectively), but TlFLU1L showed a lower K_m as compared to TpFLU12L (119.2 ± 0.02 and 170.8 ± 0.15 mg/L, respectively). ABTS significantly increased v_max to 7.73 ± 0.23 and 7.97 ± 0.28 mg/L/h, and decreased K_m to 54.8 ± 0.27 and 26.6 ± 0.21 mg/L for TlFLU1L and TpFLU12L, respectively. GC-MS analysis revealed that TlFLU1L generated metabolites 9-oxo-fluorene-1-carboxylic acid, 9H-fluoren-9-one, and phthalic acid while TpFLU12L produced 9,10-phenanthrenedione and benzene-1,2,3-tricarboxylic acid. Ecotoxicity and cytotoxicity analysis of TlFLU1L and TpFLU12L degradation products in the presence of the mediator (ABTS) are found to be non-toxic towards marine bacteria (Vibrio parahaemolyticus) and HT22 cells. Thus, the study underscores the promising potential of TlFLU1L and TpFLU12L, particularly in conjunction with mediator (ABTS), for environment friendly and efficient bioremediation of fluoranthene-contaminated environments.

由于其持久性和毒性，荧蒽对环境构成了严重威胁。研究表明，TlFLU1L（Trichoderma lixii FLU1）和TpFLU12L（Talaromyces pinophilus FLU12）可作为降解荧蒽的生物催化剂。3 U/mL的TlFLU1L和TpFLU12L可在96小时内将残留萤蒽浓度分别降至49.5 ± 8.68 %和61.0 ± 5.66 %，而10 U/mL的TlFLU1L和TpFLU12L可在96小时内将残留萤蒽浓度分别降至19.2 ± 5.95 %和28.7 ± 1.25 %。将 200 μM ABTS（2,2′-氮杂双(3-乙基苯并噻唑啉-6-磺酸)与 3 U/mL酶混合在反应中，可在 48 h 内 100%消除残留的萤石。TlFLU1L 和 TpFLU12L 的 vmax 值几乎相似（分别为 1.35 ± 0.02 和 1.29 ± 0.15 mg/L/h），但 TlFLU1L 的 Km 值低于 TpFLU12L（分别为 119.2 ± 0.02 和 170.8 ± 0.15 mg/L）。ABTS 显着提高了 TlFLU1L 和 TpFLU12L 的 vmax，分别为 7.73 ± 0.23 和 7.97 ± 0.28 mg/L/h，降低了 Km，分别为 54.8 ± 0.27 和 26.6 ± 0.21 mg/L。气相色谱-质谱分析显示，TlFLU1L 产生了代谢物 9-氧代-芴-1-羧酸、9H-芴-9-酮和邻苯二甲酸，而 TpFLU12L 则产生了 9,10-菲二酮和苯-1,2,3-三羧酸。在介质（ABTS）存在的情况下，对 TlFLU1L 和 TpFLU12L 降解产物进行生态毒性和细胞毒性分析，发现它们对海洋细菌（副溶血性弧菌）和 HT22 细胞无毒。因此，这项研究强调了 TlFLU1L 和 TpFLU12L 在环境友好型和高效生物修复受荧蒽污染的环境方面的巨大潜力，特别是在与介质（ABTS）结合使用时。

{"title":"ABTS mediator enhances biotransformation of fluoranthene by Laccases from Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12","authors":"Samson O. Egbewale , Ajit Kumar , Tosin A. Olasehinde , Mduduzi P. Mokoena , Ademola O. Olaniran","doi":"10.1016/j.ibiod.2024.105946","DOIUrl":"10.1016/j.ibiod.2024.105946","url":null,"abstract":"<div><div>Fluoranthene poses a significant environmental threat due to its persistence and toxicity. The Laccases from <em>Trichoderma lixii</em> FLU1 (<em>Tl</em>FLU1L) and <em>Talaromyces pinophilus</em> FLU12 (<em>Tp</em>FLU12L) are shown to act as biocatalysts for fluoranthene degradation. 3 U/mL of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L reduced residual fluoranthene concentration to 49.5 ± 8.68 and 61.0 ± 5.66 %, while 10U/mL to 19.2 ± 5.95 and 28.7 ± 1.25 %, respectively, in 96 h. Mixing 200 μM ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with 3 U/mL enzyme in the reaction abolished 100 % residual fluoranthene within 48 h. <em>Tl</em>FLU1L and <em>Tp</em>FLU12L exhibited almost similar <em>v</em><sub>max</sub> (1.35 ± 0.02 and 1.29 ± 0.15 mg/L/h, respectively), but <em>Tl</em>FLU1L showed a lower <em>K</em><sub>m</sub> as compared to <em>Tp</em>FLU12L (119.2 ± 0.02 and 170.8 ± 0.15 mg/L, respectively). ABTS significantly increased <em>v</em><sub>max</sub> to 7.73 ± 0.23 and 7.97 ± 0.28 mg/L/h, and decreased <em>K</em><sub>m</sub> to 54.8 ± 0.27 and 26.6 ± 0.21 mg/L for <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, respectively. GC-MS analysis revealed that <em>Tl</em>FLU1L generated metabolites 9-oxo-fluorene-1-carboxylic acid, 9H-fluoren-9-one, and phthalic acid while <em>Tp</em>FLU12L produced 9,10-phenanthrenedione and benzene-1,2,3-tricarboxylic acid. Ecotoxicity and cytotoxicity analysis of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L degradation products in the presence of the mediator (ABTS) are found to be non-toxic towards marine bacteria (<em>Vibrio parahaemolyticus</em>) and HT22 cells. Thus, the study underscores the promising potential of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, particularly in conjunction with mediator (ABTS), for environment friendly and efficient bioremediation of fluoranthene-contaminated environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105946"},"PeriodicalIF":4.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142555051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of sheep myeloid antimicrobial peptide (SMAP-18) and siderophore Pyochelin for mitigation of aerospace fuel-degrading microbes 利用羊髓抗菌肽（SMAP-18）和嗜硒酸 Pyochelin 减少航空燃料降解微生物的危害

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-10-29 DOI: 10.1016/j.ibiod.2024.105943

Amanda L. Barry Schroeder , Osman Radwan , Oscar N. Ruiz , Thusitha S. Gunasekera , Andrea Hoffmann

Sustainment of aviation fuel infrastructure is expensive and requires frequent testing for hydrocarbon-degrading microbes to assure safeguarding of fuel quality and engineering systems. Microbial contaminants in Jet fuel, including Gram-positive and Gram-negative bacteria, yeast, and filamentous fungi, necessitate early action to prevent biofouling and biocorrosion. Recent studies have identified two novel antimicrobial agents, the sheep myeloid antimicrobial peptide SMAP-18 and the iron chelating siderophore, Pyochelin with potential suitable antimicrobial properties for jet fuel sustainment. This study evaluates the antimicrobial activity of SMAP-18 and Pyochelin in specialized antimicrobial assays including liquid and fuel-culture minimum inhibitory concentration (MIC) testing, small-scale (25 mL) and large-scale (1 L) Jet A fuel microbial consortium cultures. The results show that repetitive dosing of combined SMAP-18 and Pyochelin is bactericidal and able to control bio-contaminant progression of Gram-positive Gordonia sp., and Gram-negative Pseudomonas putida bacteria in jet fuel. Moreover, the synergy observed between SMAP-18 and Pyochelin highlights their complementary mechanisms of action against microbial targets resulting in complete elimination of bacterial growth with a −7.9 log fold reduction through day 24.

航空燃料基础设施的维护费用昂贵，需要经常检测碳氢化合物降解微生物，以确保燃料质量和工程系统的安全。喷气燃料中的微生物污染物包括革兰氏阳性和革兰氏阴性细菌、酵母菌和丝状真菌，因此有必要及早采取措施防止生物污垢和生物腐蚀。最近的研究发现了两种新型抗菌剂，一种是绵羊骨髓抗菌肽 SMAP-18，另一种是铁螯合苷元 Pyochelin，这两种抗菌剂都具有潜在的适用于喷气机燃料维持的抗菌特性。本研究在专门的抗菌试验中评估了 SMAP-18 和 Pyochelin 的抗菌活性，包括液体和燃料培养物最小抑菌浓度 (MIC) 测试、小规模（25 mL）和大规模（1 L）喷气 A 型燃料微生物联合体培养物。结果表明，重复施用 SMAP-18 和 Pyochelin 复方制剂具有杀菌作用，能够控制喷气燃料中革兰氏阳性的戈登氏菌和革兰氏阴性的假单胞菌的生物污染进展。此外，在 SMAP-18 和 Pyochelin 之间观察到的协同作用突显了它们针对微生物目标的互补作用机制，从而彻底消除了细菌的生长，使其在第 24 天的生长速度降低了 -7.9 对数倍。

{"title":"Use of sheep myeloid antimicrobial peptide (SMAP-18) and siderophore Pyochelin for mitigation of aerospace fuel-degrading microbes","authors":"Amanda L. Barry Schroeder , Osman Radwan , Oscar N. Ruiz , Thusitha S. Gunasekera , Andrea Hoffmann","doi":"10.1016/j.ibiod.2024.105943","DOIUrl":"10.1016/j.ibiod.2024.105943","url":null,"abstract":"<div><div>Sustainment of aviation fuel infrastructure is expensive and requires frequent testing for hydrocarbon-degrading microbes to assure safeguarding of fuel quality and engineering systems. Microbial contaminants in Jet fuel, including Gram-positive and Gram-negative bacteria, yeast, and filamentous fungi, necessitate early action to prevent biofouling and biocorrosion. Recent studies have identified two novel antimicrobial agents, the sheep myeloid antimicrobial peptide SMAP-18 and the iron chelating siderophore, Pyochelin with potential suitable antimicrobial properties for jet fuel sustainment. This study evaluates the antimicrobial activity of SMAP-18 and Pyochelin in specialized antimicrobial assays including liquid and fuel-culture minimum inhibitory concentration (MIC) testing, small-scale (25 mL) and large-scale (1 L) Jet A fuel microbial consortium cultures. The results show that repetitive dosing of combined SMAP-18 and Pyochelin is bactericidal and able to control bio-contaminant progression of Gram-positive <em>Gordonia</em> sp., and Gram-negative <em>Pseudomonas putida</em> bacteria in jet fuel. Moreover, the synergy observed between SMAP-18 and Pyochelin highlights their complementary mechanisms of action against microbial targets resulting in complete elimination of bacterial growth with a −7.9 log fold reduction through day 24.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105943"},"PeriodicalIF":4.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142537665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0