Two Erigeron species comparison based on their ingredient profile by UPLC-PDA-QTOF-MS/MS and discriminant analysis

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2024-11-04 DOI:10.1002/rcm.9929

Jianguang Zhang, Yue Wang, Jiansang Wulu, Wenfang Jin, Qing Yang, Zhifeng Zhang

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Abstract

Rationale

Erigeron breviscapus (EB) and Erigeron multiradiatus (EM) are the two species of the genus Erigeron (Asteraceae) with extremely close genetic relationships. They were used as the same “meiduoluomi” for the treatment of plague and epidemics in traditional Tibetan medicine. But in traditional Chinese medicine, only EB is used for treatment of cerebrovascular obstruction, hemiplegia due to stroke, coronary artery obstruction, chest congestion, and angina pectoris. These two Erigeron species show different effects in different traditional medicine systems. Therefore, analyzing the chemical compositions of two species will not only enhance comprehension of their medicinal properties but also foster the advancement and exploration of novel applications. However, to date, there has been no comprehensive and detailed investigation comparing the constituents of EB and EM.

Methods

A methodology for rapid identification of chemical profiles from two Erigeron species was devised through the integration of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and multivariate statistical analysis. Additionally, a UHPLC-photo-diode array (PDA) method was established to precisely quantify of 11 components.

Results

A total of 58 constituents comprising flavonoids, phenolic acids, saponin, and long chain fatty acids were elucidated. Thirteen compounds were identified as potential differentiators in chemical profiles among the two Erigeron species. For quantitative assessment, 11 bioactive compounds were simultaneously quantified across 49 batches of Erigeron species samples utilizing UHPLC-PDA with wavelengths of 325, 254, and 266 nm. The method demonstrated excellent precision, linearity, accuracy, repeatability, stability, and recovery.

Conclusions

The findings from this study will serve as a reference for quality control, functional activity exploration, and improved clinical application based on the ingredient profiles of the two species. Furthermore, this inaugural investigation into the ingredient profiles of these two species will enhance the potential and optimal utilization of both EB and EM resources.

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通过 UPLC-PDA-QTOF-MS/MS 和判别分析，比较两种麦角菌的成分特征。

理由：Erigeron breviscapus（EB）和 Erigeron multiradiatus（EM）是菊科 Erigeron 属的两个物种，它们之间的遗传关系极为密切。在传统藏医药中，它们被作为同一种 "meiduoluomi "用于治疗瘟疫和流行病。但在传统中药中，只有乙贝用于治疗脑血管阻塞、中风偏瘫、冠状动脉阻塞、胸闷和心绞痛。这两种麦冬在不同的传统医学体系中表现出不同的功效。因此，分析这两个物种的化学成分不仅能加深对其药用特性的理解，还能促进新应用的开发和探索。然而，迄今为止，还没有对 EB 和 EM 的成分进行全面而详细的调查比较：方法：通过整合超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-QTOF-MS/MS）和多元统计分析，设计了一种快速鉴定两种艾草化学成分的方法。此外，还建立了超高效液相色谱-光电二极管阵列（PDA）方法，对 11 种成分进行精确定量：结果：共发现 58 种成分，包括黄酮类、酚酸、皂苷和长链脂肪酸。其中有 13 种化合物被确定为两种麦冬草化学成分的潜在差异成分。在定量评估方面，利用波长为 325、254 和 266 nm 的超高效液相色谱-PDA，同时对 49 个批次的麦冬样品中的 11 种生物活性化合物进行了定量分析。该方法在精密度、线性、准确度、重复性、稳定性和回收率方面均表现优异：本研究的结果将为质量控制、功能活性探索和根据两种植物的成分特征改进临床应用提供参考。此外，对这两种植物成分的首次调查将提高 EB 和 EM 资源的潜力和最佳利用率。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.