GmSTOP1-3 Increases Soybean Manganese Accumulation Under Phosphorus Deficiency by Regulating GmMATE2/13 and GmZIP6/GmIREG3.

IF 6 1区 生物学 Q1 PLANT SCIENCES Plant, Cell & Environment Pub Date : 2024-11-04 DOI:10.1111/pce.15254
Guoxuan Liu, Qianqian Chen, Dongqian Li, Huafu Mai, Yuming Zhou, Maoxin Lin, Xiaonan Feng, Xiaoying Lin, Xing Lu, Kang Chen, Jiang Tian, Cuiyue Liang
{"title":"GmSTOP1-3 Increases Soybean Manganese Accumulation Under Phosphorus Deficiency by Regulating GmMATE2/13 and GmZIP6/GmIREG3.","authors":"Guoxuan Liu, Qianqian Chen, Dongqian Li, Huafu Mai, Yuming Zhou, Maoxin Lin, Xiaonan Feng, Xiaoying Lin, Xing Lu, Kang Chen, Jiang Tian, Cuiyue Liang","doi":"10.1111/pce.15254","DOIUrl":null,"url":null,"abstract":"<p><p>Mineral nutrient deficiencies and metal ion toxicities coexist on acid soils. Phosphorus (P) deficiency in plants is generally accompanied with significant levels of leaf manganese (Mn) accumulation. However, the molecular regulatory mechanisms underpinning remain unclear. The present study found that P-deficient soybean plants accumulated more Mn compared to P-sufficient ones on acid soils in both field and greenhouse experiments. Meanwhile, both P deficiency and Mn toxicity enhanced the expression of GmSTOP1-3. Over-expressing GmSTOP1-3 enhanced Mn accumulation in transgenic soybean hairy roots, but RNA-interference did not show obvious differences. Moreover, transgenic soybeans with GmSTOP1-3-overexpression showed enhanced root citrate exudation and augmented Mn accumulation. RNA-sequence identified four downstream genes of GmSTOP1-3, including multidrug and toxic compound extrusion (GmMATE2/13) and metal transporter genes (GmZIP6/GmIREG3), which encode plasma membrane proteins. GmSTOP1-3 activated the transcription of these four genes by directly binding to their promoter regions. In addition, both GmZIP6 and GmIREG3 functioned in Mn uptake as manifested by the higher Mn concentration and decreased growth of soybean hairy roots with their overexpression. Taken together, it is suggested that upregulation of GmSTOP1-3 by low P stress on acid soils activates transcripts of GmMATE2/13 and GmZIP6/GmIREG3, which consequently result in enhanced Mn accumulation in soybean.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.15254","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Mineral nutrient deficiencies and metal ion toxicities coexist on acid soils. Phosphorus (P) deficiency in plants is generally accompanied with significant levels of leaf manganese (Mn) accumulation. However, the molecular regulatory mechanisms underpinning remain unclear. The present study found that P-deficient soybean plants accumulated more Mn compared to P-sufficient ones on acid soils in both field and greenhouse experiments. Meanwhile, both P deficiency and Mn toxicity enhanced the expression of GmSTOP1-3. Over-expressing GmSTOP1-3 enhanced Mn accumulation in transgenic soybean hairy roots, but RNA-interference did not show obvious differences. Moreover, transgenic soybeans with GmSTOP1-3-overexpression showed enhanced root citrate exudation and augmented Mn accumulation. RNA-sequence identified four downstream genes of GmSTOP1-3, including multidrug and toxic compound extrusion (GmMATE2/13) and metal transporter genes (GmZIP6/GmIREG3), which encode plasma membrane proteins. GmSTOP1-3 activated the transcription of these four genes by directly binding to their promoter regions. In addition, both GmZIP6 and GmIREG3 functioned in Mn uptake as manifested by the higher Mn concentration and decreased growth of soybean hairy roots with their overexpression. Taken together, it is suggested that upregulation of GmSTOP1-3 by low P stress on acid soils activates transcripts of GmMATE2/13 and GmZIP6/GmIREG3, which consequently result in enhanced Mn accumulation in soybean.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
GmSTOP1-3 通过调控 GmMATE2/13 和 GmZIP6/GmIREG3 增加磷缺乏条件下大豆的锰积累。
在酸性土壤中,矿质养分缺乏和金属离子毒性并存。植物磷(P)缺乏通常伴随着叶片锰(Mn)的大量积累。然而,其分子调控机制仍不清楚。本研究发现,在田间和温室实验中,缺磷大豆植株比缺磷大豆植株在酸性土壤中积累更多的锰。同时,缺磷和锰毒性都会增强 GmSTOP1-3 的表达。在转基因大豆毛根中,过表达 GmSTOP1-3 会增强锰的积累,但 RNA 干扰并未显示出明显的差异。此外,过表达 GmSTOP1-3 的转基因大豆表现出根部柠檬酸盐渗出增强和锰积累增加。通过 RNA 序列确定了 GmSTOP1-3 的四个下游基因,包括多药和有毒化合物挤出基因(GmMATE2/13)和金属转运基因(GmZIP6/GmIREG3),这些基因编码质膜蛋白。GmSTOP1-3 通过直接与这四个基因的启动子区域结合,激活了它们的转录。此外,GmZIP6 和 GmIREG3 还具有锰吸收功能,这表现在它们过量表达后,大豆毛根的锰浓度升高,生长速度降低。综上所述,酸性土壤中的低磷胁迫会上调 GmSTOP1-3 激活 GmMATE2/13 和 GmZIP6/GmIREG3 的转录本,从而增强大豆的锰积累。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
期刊最新文献
Biosynthesis-Based Spatial Metabolome of Condensed Tannin Reveals Its Role in Salt Tolerance of Non-Salt-Secretor Mangrove Kandelia obovata. A Detailed Proteomics and Metabolomics Landscape Sheds Light on the Mechanistic Insights Into the Resistance Response of Transgenic Pigeon Pea Against Wilt Stress. Issue Information Leaf Photosynthetic and Photoprotective Acclimation in the Ultraviolet-A1 and Blue Light Regions Follow a Continuous, Shallow Gradient. Warming and UV Radiation Alleviate the Effect of Virus Infection on the Microalga Emiliania huxleyi.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1