Exosomal miR-155-5p promote the occurrence of carotid atherosclerosis

Wen-Wen Yang, Qing-Xiang Li, Fei Wang, Xin-Ran Zhang, Xian-Li Zhang, Meng Wang, Dong Xue, Ying Zhao, Lu Tang
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Abstract

Periodontitis is a significant independent risk factor for atherosclerosis. Yet, the exact mechanism of action is still not fully understood. In this study, we investigated the effect of exosomes-miR-155-5p derived from periodontal endothelial cells on atherosclerosis in vitro and in vivo. Higher expression of miR-155-5p was detected in the plasma exosomes of patients with chronic periodontitis (CP) and carotid atherosclerosis (CAS) compared to patients with CP. Also, the expression level of miR-155-5p was associated with the severity of CP. miR-155-5p-enriched exosomes from HUVECs increased the angiogenesis and permeability of HAECs and promoted the expression of angiogenesis, permeability, and inflammation genes. Along with the overexpression or inhibition of miR-155-5p, the biological effect of HUVECs-derived exosomes on HAECs changed correspondingly. In ApoE−/− mouse models, miR-155-5p-enriched exosomes promoted the occurrence of carotid atherosclerosis by increasing permeable and angiogenic activity. Collectively, these findings highlight a molecular mechanism of periodontitis in CAS, uncovering exosomal miR-155-5p derived periodontitis affecting carotid endothelial cells in an ‘exosomecrine’ manner. Exosomal miR-155-5p may be used as a biomarker and target for clinical intervention to control this intractable disease in future, and the graphic abstract was shown in Figure S1.

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外泌体 miR-155-5p 促进颈动脉粥样硬化的发生
牙周炎是动脉粥样硬化的一个重要独立风险因素。然而,其确切的作用机制仍不完全清楚。在这项研究中,我们研究了从牙周内皮细胞中提取的外泌体-miR-155-5p对体外和体内动脉粥样硬化的影响。与慢性牙周炎(CP)和颈动脉粥样硬化(CAS)患者相比,在慢性牙周炎(CP)和颈动脉粥样硬化(CAS)患者的血浆外泌体中检测到更高表达的 miR-155-5p。来自 HUVECs 的 miR-155-5p 富集外泌体增加了 HAECs 的血管生成和通透性,并促进了血管生成、通透性和炎症基因的表达。随着 miR-155-5p 的过表达或抑制,HUVECs 来源的外泌体对 HAECs 的生物效应也发生了相应的变化。在载脂蛋白E-/-小鼠模型中,富含miR-155-5p的外泌体通过增加通透性和血管生成活性促进了颈动脉粥样硬化的发生。总之,这些发现凸显了中科院牙周炎的分子机制,揭示了外泌体miR-155-5p衍生的牙周炎以 "外泌体肾上腺素 "的方式影响颈动脉内皮细胞。外泌体miR-155-5p可作为生物标志物和临床干预靶点,在未来控制这种难治性疾病。
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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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