Evaluation of wound healing activity of hydromethanolic extract of Jacobaea maritima leaves in rabbits.

Abstract

The study investigated the wound healing efficacy of Jacobaea maritima leaf extract in rabbits through various wound models. A methanol-based extract was prepared from air-dried J. maritima leaves and used to formulate ointments at concentrations of 5%, 10% and 20%. Acute dermal toxicity tests confirmed no adverse reactions at the highest dose (2000mg/kg). The extract's wound healing activity was assessed using excision, incision and burn wound models. In the excision wound model, all extract concentrations significantly enhanced wound contraction compared to the control, with the 20% extract showing the highest efficacy (100% contraction by day 22). The extract also reduced epithelialization time, with the 20% formulation performing similarly to nitrofurazone. In the burn wound model, the extract ointments led to substantial wound contraction and shorter epithelialization times compared to controls, with the 20% concentration achieving 100% contraction by day 24. Histopathological examinations revealed improved healing in extract-treated wounds, with enhanced neovascularization and collagen deposition. These results suggest that J. maritima leaf extract possesses potent wound healing properties, effectively reducing healing times and enhancing tissue repair across different wound models. The study supports the potential of J.maritima as a valuable ingredient in wound care formulations.