Active mechanism of "Zishen Huayu Fang" in treating intrauterine adhesions based on network pharmacology and macromolecular docking.

Abstract

The study is investigating the mechanism of therapeutics of "Zishen Huayu Fang" in the adhesions of intrauterine. Pharmacology systems have been employed as active ingredients of 131 compounds like Luteolin, Quercetin and Kaempferol in this research study in a combination of other compounds to prove the efficacy of the Zishen Huayu Fang in the treatment of adhesions inside the uterus. The study aims to understand how the ingredients in "Zishen Huayu Fang" interact with the body, particularly how they can assist in the prevention, mitigation and elimination of adhesions inside the uterus. KEGG and GO databases and tools are used to analyze gene functions and interactions in the proposed research study. The signaling pathways such as PI3K-Akt, AGE-RAGE, HIF-1, TNF, are involved in the study to understand the critical aspects of cellular function, such as inflammation, metabolism and cell survival. This study is examining how the formula affects these crucial pathways, which could be linked to its therapeutic effects on uterine adhesions. The study suggests that "Zishen Huayu Fang" influences the expression of key targets, particularly STAT3, MAPK1, AKT1, HSP90AA1 and JUN, through active ingredients, providing a foundation for further investigation into its therapeutic efficacy in intrauterine adhesions.