Effect of Chlorella pyrenoidosa meal and Sinotaia quadrata viscera as diets on growth, digestive enzyme activities, antithrombin activity, and gut morphology of Whitmania pigra

IF 2.2 3区农林科学 Q2 FISHERIES Aquaculture International Pub Date : 2024-11-05 DOI:10.1007/s10499-024-01682-2

Yirong Wu, Jingnan Yan, Tianxin Zhuang, Lingli Jiang, Youling Gao

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Abstract

The experiment aimed to evaluate the effects of varying amounts of Chlorella pyrenoidosa meal as a supplementary diet and Sinotaia quadrata viscera on the growth, digestive enzyme activities, antithrombin activity, and gut morphology of Whitmania pigra. W. pigra (1.27 ± 0.01 g) were randomly assigned to four groups with triplicates each. The groups were fed with live S. quadrata (Snail group), live S. quadrata and 0.125 g/L C. pyrenoidosa meal (Snail + LA group), live S. quadrata and 0.250 g/L C. pyrenoidosa meal (Snail + HA group), as well as S. quadrata viscera (Snail viscera group). The feeding trial lasted for 30 days. The results showed that the final weight of Snail + HA group was significantly higher than that of Snail and Snail viscera groups, and it was significantly higher in Snail group than in Snail viscera group (P < 0.05). The trends in weight gains followed the same pattern as the body weights observed. The specific growth rate (SGR) values of Snail viscera group on days 0–15 and 0–30 were significantly lower than those of the other three groups (P < 0.05). Feed intake of the Snail + LA group was significantly higher than that of the other groups during days 0–30 (P < 0.05). The feed conversion ratio (FCR) of the Snail viscera group was inferior to that of the other three groups at both days 0–15 and 0–30 (P < 0.05). The activities of antithrombin, lipase and protease in the Snail + LA and Snail + HA groups exhibited a significant increase compared to the remaining two groups (P < 0.05). Additionally, the gene expression levels of Protease (PT) and Superoxide dismutase (SOD) in the Snail + LA and Snail + HA groups were significantly higher than those in the Snail group (P < 0.05). Moreover, the gut cell junction of the Snail visceral group appeared relatively loose, characterized by the presence of more cell vacuoles within the epithelial tissue and the occurrence of black substances in certain cells. In conclusion, both 0.125 and 0.250 g/L C. pyrenoidosa meal, when used as a supplementary diet in combination with live S. quadrata, improved the growth performance and increased antithrombin, lipase and protease activities in W. pigra. However, feeding prepared S. quadrata viscera to W. pigra adversely affected the growth and gut morphology.

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小球藻粕和褐藻内脏作为日粮对白疣梭子蟹生长、消化酶活性、抗凝血酶活性和肠道形态的影响

该实验旨在评估不同量的拟肾小球藻粉作为辅助日粮和四裂窦内脏对白豚的生长、消化酶活性、抗凝血酶活性和肠道形态的影响。将白头翁（1.27 ± 0.01 克）随机分为四组，每组三份。各组分别饲喂活的四角蜗牛（蜗牛组）、活的四角蜗牛和 0.125 克/升的焦褐藻粉（蜗牛 + LA 组）、活的四角蜗牛和 0.250 克/升的焦褐藻粉（蜗牛 + HA 组）以及四角蜗牛内脏（蜗牛内脏组）。饲喂试验持续了 30 天。结果显示，蜗牛+HA组的最终体重显著高于蜗牛组和蜗牛内脏组，蜗牛组的最终体重显著高于蜗牛内脏组（P < 0.05）。体重增加的趋势与观察到的体重变化趋势相同。蜗牛内脏组在第 0-15 天和第 0-30 天的特定生长率（SGR）值明显低于其他三个组（P < 0.05）。蜗牛+LA组在第0-30天的采食量明显高于其他各组（P <0.05）。蜗牛内脏组的饲料转化率（FCR）在第 0-15 天和第 0-30 天均低于其他三组（P < 0.05）。与其余两组相比，蜗牛 + LA 组和蜗牛 + HA 组的抗凝血酶、脂肪酶和蛋白酶活性均有显著提高（P < 0.05）。此外，蜗牛 + LA 组和蜗牛 + HA 组的蛋白酶（PT）和超氧化物歧化酶（SOD）的基因表达水平明显高于蜗牛组（P < 0.05）。此外，蜗牛内脏组的肠道细胞交界处显得相对疏松，其特点是上皮组织内存在较多的细胞空泡，某些细胞内出现黑色物质。总之，0.125 和 0.250 克/升的蛇床子粉与活的 S. quadrata 一起用作补充饲料时，都能改善猪蜗牛的生长性能并提高其抗凝血酶、脂肪酶和蛋白酶活性。然而，用制备好的 S. quadrata 内脏饲喂小袋鼠会对其生长和肠道形态产生不利影响。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.