Mitigating effect of Bifidobacterium longum CCFM1077 on nonylphenol toxicity: An integrative in vitro and in vivo analysis

Abstract

Nonylphenol (NP), an endocrine-disrupting compound (EDC) with accumulative properties, poses significant risks to human health and the environment. The pivotal role of probiotics in mitigating EDC toxicity has garnered increasing attention. In this study, we assessed the protective effects of Bifidobacterium longum CCFM1077, a probiotic with outstanding in vitro NP-binding ability, against NP-induced toxicity in rats. This analysis revealed that B. longum CCFM1077 effectively promoted the NP excretion and enhanced intestinal barrier integrity. Interestingly, B. longum CCFM1077, by modulating the structure and the function of gut microbiota, increased the abundance of Turicibacter, significantly elevated the level of butyric acid, and upregulated antioxidant-related metabolic pathways, thereby alleviating brain inflammation and ultimately improving behavioral disorders. This study elucidated a strategy to alleviate NP toxicity and lays a theoretical foundation for the development of novel intestinal protection strategies. It supports environmental sustainability by offering a strategy to combat NP bioaccumulation, aligning with global initiatives to minimize the environmental impact of industrial pollutants.