Metallic-based phthalocyanine nanoemulsions for photodynamic purging of ovarian tissue in leukemia patients.

IF 5.4 2区医学 Q1 BIOPHYSICS Colloids and Surfaces B: Biointerfaces Pub Date : 2024-10-24 DOI:10.1016/j.colsurfb.2024.114338

Saeid Moghassemi, Arezoo Dadashzadeh, Saba Nikanfar, Pejman Ghaffari-Bohlouli, Paulo Eduardo Narcizo de Souza, Amin Shavandi, Ricardo Bentes de Azevedo, Christiani A Amorim

{"title":"Metallic-based phthalocyanine nanoemulsions for photodynamic purging of ovarian tissue in leukemia patients.","authors":"Saeid Moghassemi, Arezoo Dadashzadeh, Saba Nikanfar, Pejman Ghaffari-Bohlouli, Paulo Eduardo Narcizo de Souza, Amin Shavandi, Ricardo Bentes de Azevedo, Christiani A Amorim","doi":"10.1016/j.colsurfb.2024.114338","DOIUrl":null,"url":null,"abstract":"<p><p>For cancer patients with a high risk of ovarian tissue metastasis, ovarian autotransplantation is not advised due to the potential spread of malignant cells. Ex vivo purging of ovarian fragments may offer a more suitable alternative for fertility restoration. Eradicating malignant cells should be done selectively without affecting follicles or ovarian stromal cells (SCs). As a clinically licensed method, photodynamic therapy (PDT) is a minimally invasive treatment to destroy cancer cells. This study evaluates the effectiveness of nanoemulsions (NE) containing two phthalocyanine photosensitizers; aluminum (III) phthalocyanine (AlPc) and zinc (II) phthalocyanine (ZnPc) in eliminating cancer cells. Human leukemic malignant (HL-60) and ovarian stromal cells (SCs) were treated with AlPc/ZnPc loaded NEs with or without diode laser irradiation. HL-60 leukemia cells in 2D culture were eliminated by treatment with 10 nM AlPc-NE or 0.1 µM ZnPc-NE, while no toxicity was observed in SCs. In 3D culture models, although the cells showed more resistance to the NEs as a result of limited oxygen and photosensitizer penetration, the treatment remained selective for cancer cells. These approaches have the potential to eliminate malignant cells from ovarian tissue fragments.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114338"},"PeriodicalIF":5.4000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Surfaces B: Biointerfaces","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1016/j.colsurfb.2024.114338","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

For cancer patients with a high risk of ovarian tissue metastasis, ovarian autotransplantation is not advised due to the potential spread of malignant cells. Ex vivo purging of ovarian fragments may offer a more suitable alternative for fertility restoration. Eradicating malignant cells should be done selectively without affecting follicles or ovarian stromal cells (SCs). As a clinically licensed method, photodynamic therapy (PDT) is a minimally invasive treatment to destroy cancer cells. This study evaluates the effectiveness of nanoemulsions (NE) containing two phthalocyanine photosensitizers; aluminum (III) phthalocyanine (AlPc) and zinc (II) phthalocyanine (ZnPc) in eliminating cancer cells. Human leukemic malignant (HL-60) and ovarian stromal cells (SCs) were treated with AlPc/ZnPc loaded NEs with or without diode laser irradiation. HL-60 leukemia cells in 2D culture were eliminated by treatment with 10 nM AlPc-NE or 0.1 µM ZnPc-NE, while no toxicity was observed in SCs. In 3D culture models, although the cells showed more resistance to the NEs as a result of limited oxygen and photosensitizer penetration, the treatment remained selective for cancer cells. These approaches have the potential to eliminate malignant cells from ovarian tissue fragments.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

用于白血病患者卵巢组织光动力净化的金属基酞菁纳米乳剂。

对于卵巢组织转移风险较高的癌症患者，由于恶性细胞可能扩散，不建议进行卵巢自体移植。卵巢碎片的体外清除可能是恢复生育能力的一个更合适的选择。清除恶性细胞应在不影响卵泡或卵巢基质细胞（SC）的情况下有选择地进行。作为一种临床许可的方法，光动力疗法（PDT）是一种消灭癌细胞的微创疗法。本研究评估了含有两种酞菁光敏剂（铝（III）酞菁（AlPc）和锌（II）酞菁（ZnPc））的纳米乳剂（NE）在消灭癌细胞方面的效果。在使用或不使用二极管激光照射的情况下，使用负载 AlPc/ZnPc 的 NE 处理人类恶性白血病细胞（HL-60）和卵巢基质细胞（SCs）。用 10 nM AlPc-NE 或 0.1 µM ZnPc-NE 处理二维培养模型中的 HL-60 白血病细胞会使其死亡，而在 SCs 中则未观察到毒性。在三维培养模型中，虽然由于氧气和光敏剂渗透有限，细胞对NE表现出更大的抵抗力，但治疗对癌细胞仍有选择性。这些方法有望消除卵巢组织碎片中的恶性细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.