Electrospun nanofibrous scaffolds reinforced with therapeutic lithium/manganese-doped calcium phosphates: Advancing skin cancer therapy through apoptosis induction.

IF 5.4 2区 医学 Q1 BIOPHYSICS Colloids and Surfaces B: Biointerfaces Pub Date : 2024-10-31 DOI:10.1016/j.colsurfb.2024.114348
Sara Gorgani, Farzad Kermani, Khatereh Sadeghzadeh, Arghavan Vojdani, Sara Hooshmand, Kobra Foroughi, Zoleikha Azari, Seyede Atefe Hosseini, Sahar Mollazadeh, Alireza Ebrahimzadeh Bideskan, Simin Nazarnezhad
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Abstract

In the current study we fabricated potent materials by incorporating therapeutic elements into calcium phosphates (CPs) to combat cancer. This involved synthesizing manganese (Mn)- and lithium (Li)-doped CPs and loading them into electrospun nanofibers (NFs) composed of chitosan (CS) and polyethylene oxide (PEO). The characterized CPs exhibited excellent properties, including a particle size of 47-75 nm, surface charge of -(30-56) mV, and specific surface area of 75-266 m2/g. The electrochemical analysis revealed that Mn and Mn/Li-doped CPs are promising for generating oxygen free radicals and H2O2, crucial for cancer therapy. Biological evaluation showcased the outstanding performance of the developed materials. MTT assay revealed a cytotoxic effect of nano-constructs on melanoma A375 cell line without adverse effects on normal L929 cells over 72 h. Annexin V/PI apoptosis assay indicated substantial apoptosis rates in A375 cells treated with PC-20 % (62.55 ± 4.59 %). The obtained data of qPCR analysis of pro-apoptotic and anti-apoptotic genes (P53, Bax, Bcl-2) in A375 cells treated with different CP nanoparticles (NPs) showed a significant increase in P53 and Bax gene expression, indicating high levels of A375 cell apoptosis. Additionally, the samples containing Mn ion exhibited high reactive oxygen species (ROS) generation. In conclusion, the fabricated NFs scaffolds hold promising potential for cancer therapy.

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用治疗性锂/锰掺杂磷酸钙增强的电纺纳米纤维支架:通过诱导细胞凋亡推进皮肤癌治疗。
在目前的研究中,我们通过在磷酸钙(CPs)中加入治疗元素,制造出了有效的抗癌材料。这包括合成掺杂锰(Mn)和锂(Li)的磷酸钙,并将其装入由壳聚糖(CS)和聚环氧乙烷(PEO)组成的电纺纳米纤维(NF)中。所表征的氯化石蜡具有优异的性能,包括粒径为 47-75 nm,表面电荷为 -(30-56) mV,比表面积为 75-266 m2/g。电化学分析表明,锰和锰/锂掺杂的氯化石蜡有望产生氧自由基和 H2O2,这对癌症治疗至关重要。生物学评估显示了所开发材料的卓越性能。MTT 检测显示,纳米结构对黑色素瘤 A375 细胞株有细胞毒性作用,72 小时内对正常 L929 细胞无不良影响。Annexin V/PI 细胞凋亡检测表明,用 PC-20 % 处理的 A375 细胞凋亡率很高(62.55 ± 4.59 %)。不同 CP 纳米粒子(NPs)处理的 A375 细胞中促凋亡基因和抗凋亡基因(P53、Bax、Bcl-2)的 qPCR 分析数据显示,P53 和 Bax 基因表达量显著增加,表明 A375 细胞凋亡水平很高。此外,含有锰离子的样品表现出较高的活性氧(ROS)生成。总之,所制备的 NFs 支架在癌症治疗方面具有广阔的前景。
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来源期刊
Colloids and Surfaces B: Biointerfaces
Colloids and Surfaces B: Biointerfaces 生物-材料科学:生物材料
CiteScore
11.10
自引率
3.40%
发文量
730
审稿时长
42 days
期刊介绍: Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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