{"title":"Nickel-doped cuprous oxide nanocauliflowers with specific peroxidase-like activity for sensitive detection of hydrogen peroxide and uric acid.","authors":"Rou Cheng, Zhengyue Xiao, Xiaomin Tang, Peng Xu, Ping Qiu","doi":"10.1016/j.colsurfb.2024.114347","DOIUrl":null,"url":null,"abstract":"<p><p>Copper-based nanomaterials have the properties of mimetic enzymes and can be used as excellent candidates for colorimetric sensing due to their environmental friendliness, low cost, and high abundance. In this paper, Ni-doped Cu<sub>2</sub>O nano cauliflower (Ni-Cu<sub>2</sub>O) was synthesized for the first time and applied to the detection of H<sub>2</sub>O<sub>2</sub> and uric acid (UA) in human serum and urine. It was found that the proportion of Ni incorporation controls the morphology and the catalytic effect of Ni-Cu<sub>2</sub>O. The catalytic mechanism was studied by X-ray photoelectron spectroscopy, free radical capture experiments, photoluminescence spectroscopy, and steady-state kinetic analysis, which verified the redox reactions involving electron transfer and active substances. The results showed that Ni-Cu<sub>2</sub>O could catalyze the formation of reactive oxygen species (•OH, O<sub>2</sub><sup>•-</sup>, <sub>1</sub>O<sup>2</sup>, h<sup>+</sup>) from H<sub>2</sub>O<sub>2</sub>, which could oxidize 3,3', 5,5'-tetramethylbenzidine (TMB) to oxTMB, and the color changed from colorless to blue. The Michaelis-Menten constant (K<sub>m</sub>) and the maximum initial velocity (V<sub>max</sub>) of Ni-Cu<sub>2</sub>O were 1.8 mM and 15.2×10<sup>-8</sup> M/s, respectively. Based on the excellent peroxidase-like (POD) activity of Ni-Cu<sub>2</sub>O, a colorimetric sensing platform combined with TMB was proposed to sensitively detect H<sub>2</sub>O<sub>2</sub> and UA in a wide range, and the detection limits were as low as 0.17 μM and 0.22 μM, respectively. This study creates a platform for using the Cu-based cauliflowers as a biosensor to detect UA in the medical and biomedicine fields.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Surfaces B: Biointerfaces","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1016/j.colsurfb.2024.114347","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0
Abstract
Copper-based nanomaterials have the properties of mimetic enzymes and can be used as excellent candidates for colorimetric sensing due to their environmental friendliness, low cost, and high abundance. In this paper, Ni-doped Cu2O nano cauliflower (Ni-Cu2O) was synthesized for the first time and applied to the detection of H2O2 and uric acid (UA) in human serum and urine. It was found that the proportion of Ni incorporation controls the morphology and the catalytic effect of Ni-Cu2O. The catalytic mechanism was studied by X-ray photoelectron spectroscopy, free radical capture experiments, photoluminescence spectroscopy, and steady-state kinetic analysis, which verified the redox reactions involving electron transfer and active substances. The results showed that Ni-Cu2O could catalyze the formation of reactive oxygen species (•OH, O2•-, 1O2, h+) from H2O2, which could oxidize 3,3', 5,5'-tetramethylbenzidine (TMB) to oxTMB, and the color changed from colorless to blue. The Michaelis-Menten constant (Km) and the maximum initial velocity (Vmax) of Ni-Cu2O were 1.8 mM and 15.2×10-8 M/s, respectively. Based on the excellent peroxidase-like (POD) activity of Ni-Cu2O, a colorimetric sensing platform combined with TMB was proposed to sensitively detect H2O2 and UA in a wide range, and the detection limits were as low as 0.17 μM and 0.22 μM, respectively. This study creates a platform for using the Cu-based cauliflowers as a biosensor to detect UA in the medical and biomedicine fields.
期刊介绍：
Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields.
Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication.
The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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