Scaling up production of cephalosporin C by Acremonium chrysogenum W42-I in a fermenter using submerged fermentation.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-11-05 DOI:10.1186/s13568-024-01778-1
Asmaa A Ibrahim, Ghadir S El-Housseiny, Khaled M Aboshanab, Ansgar Stratmann, Mahmoud A Yassien, Nadia A Hassouna
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Abstract

Cephalosporins presently stand as the most extensively utilized antibiotic in clinical settings. Acremonium (A.) chrysogenum is the main strain used in the manufacturing of cephalosporin C (CPC), which offers distinct advantages, including a wide-ranging antibacterial spectrum and powerful antibacterial efficacy. Our study aimed to determine the optimal conditions for scaling up the production of CPC from A. chrysogenum W42-I starting with the optimized conditions on the shake flask level obtained from our previous study and utilizing the optimized media (CPC2). The results indicated that an inoculum size equivalent to 1% v/v, aeration at 1 vvm, and an agitation rate of 400 rpm, with controlled pH at 4, were the most favorable conditions for the CPC production using a laboratory fermentor (14 L). The concentration of generated CPC was assessed using two standard curves obtained from agar well diffusion and high-performance liquid chromatography (HPLC). These optimized conditions resulted in a production of 399.52 µg/mL showing a significant increase of approximately 3.4 folds when compared to the unoptimized fermentation run. In conclusion, our findings demonstrated a more favorable time course for CPC production in the fermentor compared to that in the shake flask. Notably, there was a two-fold increase in production within the first three days. Fortunately, the fermentor achieved a noteworthy increase in output, generating 1.598 gm of the CPC within 4 L.

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在发酵罐中利用浸没式发酵法扩大 Acremonium chrysogenum W42-I 生产头孢菌素 C 的规模。
头孢菌素是目前临床应用最广泛的抗生素。Acremonium (A.) chrysogenum 是生产头孢菌素 C(CPC)的主要菌株,它具有独特的优势,包括抗菌谱广和抗菌效力强。我们的研究旨在确定从 A. chrysogenum W42-I 菌株中扩大 CPC 生产规模的最佳条件,从我们之前研究中获得的摇瓶水平优化条件开始,并利用优化培养基(CPC2)。结果表明,接种物大小相当于 1%v/v、通气量为 1 vvm、搅拌速率为 400 rpm、pH 值控制在 4 是使用实验室发酵罐(14 升)生产 CPC 的最有利条件。利用琼脂井扩散和高效液相色谱法 (HPLC) 得出的两条标准曲线对生成的 CPC 浓度进行了评估。优化条件下的产量为 399.52 µg/mL,与未优化的发酵运行相比,显著增加了约 3.4 倍。总之,我们的研究结果表明,与摇瓶相比,在发酵罐中生产 CPC 的时间过程更为有利。值得注意的是,头三天的产量增加了两倍。幸运的是,发酵罐的产量显著增加,在 4 升的发酵罐中产生了 1.598 克 CPC。
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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