{"title":"Relationship between oriT length and efficiency of RP4-mediated conjugation from E. coli to gram-positive bacteria.","authors":"Yuta Fukada, Shunsuke Inomata, Masakazu Kataoka","doi":"10.1093/bbb/zbae159","DOIUrl":null,"url":null,"abstract":"<p><p>In RP4 conjugation, approximately 350 bp of the origin of transfer (oriT) is required for transfer. Within this oriT, there are binding regions for the transfer-related proteins TraI, TraK, and TraJ. We investigated the influence of deleting each protein binding region within oriT on transfer efficiency in E. coli, S. lividans, and B. subtilis. The deletion of the TraI binding region completely abolished transfer in all species. The partial deletion of the TraK binding region had a minimal impact when targeting but affected efficiency when targeting B. subtilis. The deletion of the TraJ binding region completely abolished transfer in E. coli and B. subtilis but only reduced efficiency in S. lividans. This is the first report to investigate the influence of each region within oriT on transfer efficiency in S. lividans and B. subtilis, suggesting that the length of oriT required for effective RP4 conjugation varies when targeting Gram-positive bacteria.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/bbb/zbae159","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
In RP4 conjugation, approximately 350 bp of the origin of transfer (oriT) is required for transfer. Within this oriT, there are binding regions for the transfer-related proteins TraI, TraK, and TraJ. We investigated the influence of deleting each protein binding region within oriT on transfer efficiency in E. coli, S. lividans, and B. subtilis. The deletion of the TraI binding region completely abolished transfer in all species. The partial deletion of the TraK binding region had a minimal impact when targeting but affected efficiency when targeting B. subtilis. The deletion of the TraJ binding region completely abolished transfer in E. coli and B. subtilis but only reduced efficiency in S. lividans. This is the first report to investigate the influence of each region within oriT on transfer efficiency in S. lividans and B. subtilis, suggesting that the length of oriT required for effective RP4 conjugation varies when targeting Gram-positive bacteria.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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